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IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen

We have previously developed a methodology to produce protein microspheres (MS) that can be loaded with proteins of interest in living cells through their C or N-terminal tagging with the so-called IC-Tag. The IC-Tagging method has many applications ranging from the production of immobilized enzymes...

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Autores principales: Barreiro-Piñeiro, Natalia, Lostalé-Seijo, Irene, Varela-Calviño, Rubén, Benavente, Javier, Martínez-Costas, José M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214907/
https://www.ncbi.nlm.nih.gov/pubmed/30390011
http://dx.doi.org/10.1038/s41598-018-34488-3
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author Barreiro-Piñeiro, Natalia
Lostalé-Seijo, Irene
Varela-Calviño, Rubén
Benavente, Javier
Martínez-Costas, José M.
author_facet Barreiro-Piñeiro, Natalia
Lostalé-Seijo, Irene
Varela-Calviño, Rubén
Benavente, Javier
Martínez-Costas, José M.
author_sort Barreiro-Piñeiro, Natalia
collection PubMed
description We have previously developed a methodology to produce protein microspheres (MS) that can be loaded with proteins of interest in living cells through their C or N-terminal tagging with the so-called IC-Tag. The IC-Tagging method has many applications ranging from the production of immobilized enzymes for industrial use to the production of subunit vaccines due to its intrinsic adjuvancy. Here we show the adaptation of the IC-Tagging to work inside the endoplasmic reticulum and bacteria, allowing us to produce properly modified viral glycoproteins. Additionally, we were able to express the Islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP), whose expression remained elusive to date possibly due to its toxicity when over-expressed. IGRP is an antigen of enormous pharmaceutical interest as it is specifically targeted during the autoimmune response taking place in both the Non-Obese Diabetic (NOD) mice and type 1 diabetes (T1D) patients leading to the destruction of insulin-producing beta cells.
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spelling pubmed-62149072018-11-06 IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen Barreiro-Piñeiro, Natalia Lostalé-Seijo, Irene Varela-Calviño, Rubén Benavente, Javier Martínez-Costas, José M. Sci Rep Article We have previously developed a methodology to produce protein microspheres (MS) that can be loaded with proteins of interest in living cells through their C or N-terminal tagging with the so-called IC-Tag. The IC-Tagging method has many applications ranging from the production of immobilized enzymes for industrial use to the production of subunit vaccines due to its intrinsic adjuvancy. Here we show the adaptation of the IC-Tagging to work inside the endoplasmic reticulum and bacteria, allowing us to produce properly modified viral glycoproteins. Additionally, we were able to express the Islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP), whose expression remained elusive to date possibly due to its toxicity when over-expressed. IGRP is an antigen of enormous pharmaceutical interest as it is specifically targeted during the autoimmune response taking place in both the Non-Obese Diabetic (NOD) mice and type 1 diabetes (T1D) patients leading to the destruction of insulin-producing beta cells. Nature Publishing Group UK 2018-11-02 /pmc/articles/PMC6214907/ /pubmed/30390011 http://dx.doi.org/10.1038/s41598-018-34488-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Barreiro-Piñeiro, Natalia
Lostalé-Seijo, Irene
Varela-Calviño, Rubén
Benavente, Javier
Martínez-Costas, José M.
IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen
title IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen
title_full IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen
title_fullStr IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen
title_full_unstemmed IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen
title_short IC-Tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound IGRP autoantigen
title_sort ic-tagging methodology applied to the expression of viral glycoproteins and the difficult-to-express membrane-bound igrp autoantigen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214907/
https://www.ncbi.nlm.nih.gov/pubmed/30390011
http://dx.doi.org/10.1038/s41598-018-34488-3
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