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An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages
Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214992/ https://www.ncbi.nlm.nih.gov/pubmed/30389997 http://dx.doi.org/10.1038/s41598-018-34524-2 |
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author | Beneke, Valerie Küster, Fennja Neehus, Anna-Lena Hesse, Christina Lopez-Rodriguez, Elena Haake, Kathrin Rafiei Hashtchin, Anna Schott, Juliane Wilhelmine Walter, Dorothee Braun, Armin Wolkers, Willem F. Ackermann, Mania Lachmann, Nico |
author_facet | Beneke, Valerie Küster, Fennja Neehus, Anna-Lena Hesse, Christina Lopez-Rodriguez, Elena Haake, Kathrin Rafiei Hashtchin, Anna Schott, Juliane Wilhelmine Walter, Dorothee Braun, Armin Wolkers, Willem F. Ackermann, Mania Lachmann, Nico |
author_sort | Beneke, Valerie |
collection | PubMed |
description | Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform. |
format | Online Article Text |
id | pubmed-6214992 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62149922018-11-06 An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages Beneke, Valerie Küster, Fennja Neehus, Anna-Lena Hesse, Christina Lopez-Rodriguez, Elena Haake, Kathrin Rafiei Hashtchin, Anna Schott, Juliane Wilhelmine Walter, Dorothee Braun, Armin Wolkers, Willem F. Ackermann, Mania Lachmann, Nico Sci Rep Article Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform. Nature Publishing Group UK 2018-11-02 /pmc/articles/PMC6214992/ /pubmed/30389997 http://dx.doi.org/10.1038/s41598-018-34524-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Beneke, Valerie Küster, Fennja Neehus, Anna-Lena Hesse, Christina Lopez-Rodriguez, Elena Haake, Kathrin Rafiei Hashtchin, Anna Schott, Juliane Wilhelmine Walter, Dorothee Braun, Armin Wolkers, Willem F. Ackermann, Mania Lachmann, Nico An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages |
title | An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages |
title_full | An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages |
title_fullStr | An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages |
title_full_unstemmed | An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages |
title_short | An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages |
title_sort | immune cell spray (ics) formulation allows for the delivery of functional monocyte/macrophages |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214992/ https://www.ncbi.nlm.nih.gov/pubmed/30389997 http://dx.doi.org/10.1038/s41598-018-34524-2 |
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