Cargando…

An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages

Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose...

Descripción completa

Detalles Bibliográficos
Autores principales: Beneke, Valerie, Küster, Fennja, Neehus, Anna-Lena, Hesse, Christina, Lopez-Rodriguez, Elena, Haake, Kathrin, Rafiei Hashtchin, Anna, Schott, Juliane Wilhelmine, Walter, Dorothee, Braun, Armin, Wolkers, Willem F., Ackermann, Mania, Lachmann, Nico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214992/
https://www.ncbi.nlm.nih.gov/pubmed/30389997
http://dx.doi.org/10.1038/s41598-018-34524-2
_version_ 1783368053764915200
author Beneke, Valerie
Küster, Fennja
Neehus, Anna-Lena
Hesse, Christina
Lopez-Rodriguez, Elena
Haake, Kathrin
Rafiei Hashtchin, Anna
Schott, Juliane Wilhelmine
Walter, Dorothee
Braun, Armin
Wolkers, Willem F.
Ackermann, Mania
Lachmann, Nico
author_facet Beneke, Valerie
Küster, Fennja
Neehus, Anna-Lena
Hesse, Christina
Lopez-Rodriguez, Elena
Haake, Kathrin
Rafiei Hashtchin, Anna
Schott, Juliane Wilhelmine
Walter, Dorothee
Braun, Armin
Wolkers, Willem F.
Ackermann, Mania
Lachmann, Nico
author_sort Beneke, Valerie
collection PubMed
description Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform.
format Online
Article
Text
id pubmed-6214992
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-62149922018-11-06 An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages Beneke, Valerie Küster, Fennja Neehus, Anna-Lena Hesse, Christina Lopez-Rodriguez, Elena Haake, Kathrin Rafiei Hashtchin, Anna Schott, Juliane Wilhelmine Walter, Dorothee Braun, Armin Wolkers, Willem F. Ackermann, Mania Lachmann, Nico Sci Rep Article Macrophages are key cells of the innate immune system and act as tissue resident macrophages (TRMs) in the homeostasis of various tissues. Given their unique functions and therapeutic use as well as the feasibility to derive macrophages in vitro from hematopoietic stem cell (HSC) sources, we propose an “easy-to-use” immune cell spray (ICS) formulation to effectively deliver HSC-derived macrophages. To achieve this aim, we used classical pump spray devices to spray either the human myeloid cell line U937 or primary murine HSC-derived macrophages. For both cell types used, one puff could deliver cells with maintained morphology and functionality. Of note, cells tolerated the spraying process very well with a recovery of more than 90%. In addition, we used osmotic preconditioning to reduce the overall cell size of macrophages. While a 800 mosm hyperosmolar sucrose solution was able to reduce the cell size by 27%, we identified 600 mosm to be effective to reduce the cell size by 15% while maintaining macrophage morphology and functionality. Using an isolated perfused rat lung preparation, the combinatorial use of the ICS with preconditioned and genetically labeled U937 cells allowed the intra-pulmonary delivery of cells, thus paving the way for a new cell delivery platform. Nature Publishing Group UK 2018-11-02 /pmc/articles/PMC6214992/ /pubmed/30389997 http://dx.doi.org/10.1038/s41598-018-34524-2 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Beneke, Valerie
Küster, Fennja
Neehus, Anna-Lena
Hesse, Christina
Lopez-Rodriguez, Elena
Haake, Kathrin
Rafiei Hashtchin, Anna
Schott, Juliane Wilhelmine
Walter, Dorothee
Braun, Armin
Wolkers, Willem F.
Ackermann, Mania
Lachmann, Nico
An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages
title An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages
title_full An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages
title_fullStr An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages
title_full_unstemmed An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages
title_short An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages
title_sort immune cell spray (ics) formulation allows for the delivery of functional monocyte/macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214992/
https://www.ncbi.nlm.nih.gov/pubmed/30389997
http://dx.doi.org/10.1038/s41598-018-34524-2
work_keys_str_mv AT benekevalerie animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT kusterfennja animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT neehusannalena animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT hessechristina animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT lopezrodriguezelena animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT haakekathrin animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT rafieihashtchinanna animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT schottjulianewilhelmine animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT walterdorothee animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT braunarmin animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT wolkerswillemf animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT ackermannmania animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT lachmannnico animmunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT benekevalerie immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT kusterfennja immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT neehusannalena immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT hessechristina immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT lopezrodriguezelena immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT haakekathrin immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT rafieihashtchinanna immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT schottjulianewilhelmine immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT walterdorothee immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT braunarmin immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT wolkerswillemf immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT ackermannmania immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages
AT lachmannnico immunecellsprayicsformulationallowsforthedeliveryoffunctionalmonocytemacrophages