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Cytotoxic, Genotoxic, and Polymorphism Effects on Vanilla planifolia Jacks ex Andrews after Long-Term Exposure to Argovit(®) Silver Nanoparticles

Worldwide demands of Vanilla planifolia lead to finding new options to produce large-scale and contaminant-free crops. Particularly, the Mexican Government has classified Vanilla planifolia at risk and it subject to protection programs since wild species are in danger of extinction and no more than...

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Autores principales: Bello-Bello, Jericó Jabín, Spinoso-Castillo, José Luis, Arano-Avalos, Samantha, Martínez-Estrada, Eduardo, Arellano-García, María Evarista, Pestryakov, Alexey, Toledano-Magaña, Yanis, García-Ramos, Juan Carlos, Bogdanchikova, Nina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215222/
https://www.ncbi.nlm.nih.gov/pubmed/30257465
http://dx.doi.org/10.3390/nano8100754
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author Bello-Bello, Jericó Jabín
Spinoso-Castillo, José Luis
Arano-Avalos, Samantha
Martínez-Estrada, Eduardo
Arellano-García, María Evarista
Pestryakov, Alexey
Toledano-Magaña, Yanis
García-Ramos, Juan Carlos
Bogdanchikova, Nina
author_facet Bello-Bello, Jericó Jabín
Spinoso-Castillo, José Luis
Arano-Avalos, Samantha
Martínez-Estrada, Eduardo
Arellano-García, María Evarista
Pestryakov, Alexey
Toledano-Magaña, Yanis
García-Ramos, Juan Carlos
Bogdanchikova, Nina
author_sort Bello-Bello, Jericó Jabín
collection PubMed
description Worldwide demands of Vanilla planifolia lead to finding new options to produce large-scale and contaminant-free crops. Particularly, the Mexican Government has classified Vanilla planifolia at risk and it subject to protection programs since wild species are in danger of extinction and no more than 30 clones have been found. Nanotechnology could help to solve both demands and genetic variability, but toxicological concerns must be solved. In this work, we present the first study of the cytotoxic and genotoxic effects promoted by AgNPs in Vanilla planifolia plantlets after a very long exposure time of six weeks. Our results show that Vanilla planifolia plantlets growth with doses of 25 and 50 mg/L is favored with a small decrease in the mitotic index. A dose-dependency in the frequency of cells with chromosomal aberrations and micronuclei was found. However, genotoxic effects could be considered as minimum due to with the highest concentration employed (200 mg/L), the total percentage of chromatic aberrations is lower than 5% with only three micronuclei in 3000 cells, despite the long-time exposure to AgNP. Therefore, 25 and 50 mg/L (1.5 and 3 mg/L of metallic silver) were identified as safe concentrations for Vanilla planifolia growth on in vitro conditions. Exposure of plantlets to AgNPs increase the polymorphism registered by inter-simple sequence repeat method (ISSR), which could be useful to promote the genetic variability of this species.
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spelling pubmed-62152222018-11-14 Cytotoxic, Genotoxic, and Polymorphism Effects on Vanilla planifolia Jacks ex Andrews after Long-Term Exposure to Argovit(®) Silver Nanoparticles Bello-Bello, Jericó Jabín Spinoso-Castillo, José Luis Arano-Avalos, Samantha Martínez-Estrada, Eduardo Arellano-García, María Evarista Pestryakov, Alexey Toledano-Magaña, Yanis García-Ramos, Juan Carlos Bogdanchikova, Nina Nanomaterials (Basel) Article Worldwide demands of Vanilla planifolia lead to finding new options to produce large-scale and contaminant-free crops. Particularly, the Mexican Government has classified Vanilla planifolia at risk and it subject to protection programs since wild species are in danger of extinction and no more than 30 clones have been found. Nanotechnology could help to solve both demands and genetic variability, but toxicological concerns must be solved. In this work, we present the first study of the cytotoxic and genotoxic effects promoted by AgNPs in Vanilla planifolia plantlets after a very long exposure time of six weeks. Our results show that Vanilla planifolia plantlets growth with doses of 25 and 50 mg/L is favored with a small decrease in the mitotic index. A dose-dependency in the frequency of cells with chromosomal aberrations and micronuclei was found. However, genotoxic effects could be considered as minimum due to with the highest concentration employed (200 mg/L), the total percentage of chromatic aberrations is lower than 5% with only three micronuclei in 3000 cells, despite the long-time exposure to AgNP. Therefore, 25 and 50 mg/L (1.5 and 3 mg/L of metallic silver) were identified as safe concentrations for Vanilla planifolia growth on in vitro conditions. Exposure of plantlets to AgNPs increase the polymorphism registered by inter-simple sequence repeat method (ISSR), which could be useful to promote the genetic variability of this species. MDPI 2018-09-25 /pmc/articles/PMC6215222/ /pubmed/30257465 http://dx.doi.org/10.3390/nano8100754 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bello-Bello, Jericó Jabín
Spinoso-Castillo, José Luis
Arano-Avalos, Samantha
Martínez-Estrada, Eduardo
Arellano-García, María Evarista
Pestryakov, Alexey
Toledano-Magaña, Yanis
García-Ramos, Juan Carlos
Bogdanchikova, Nina
Cytotoxic, Genotoxic, and Polymorphism Effects on Vanilla planifolia Jacks ex Andrews after Long-Term Exposure to Argovit(®) Silver Nanoparticles
title Cytotoxic, Genotoxic, and Polymorphism Effects on Vanilla planifolia Jacks ex Andrews after Long-Term Exposure to Argovit(®) Silver Nanoparticles
title_full Cytotoxic, Genotoxic, and Polymorphism Effects on Vanilla planifolia Jacks ex Andrews after Long-Term Exposure to Argovit(®) Silver Nanoparticles
title_fullStr Cytotoxic, Genotoxic, and Polymorphism Effects on Vanilla planifolia Jacks ex Andrews after Long-Term Exposure to Argovit(®) Silver Nanoparticles
title_full_unstemmed Cytotoxic, Genotoxic, and Polymorphism Effects on Vanilla planifolia Jacks ex Andrews after Long-Term Exposure to Argovit(®) Silver Nanoparticles
title_short Cytotoxic, Genotoxic, and Polymorphism Effects on Vanilla planifolia Jacks ex Andrews after Long-Term Exposure to Argovit(®) Silver Nanoparticles
title_sort cytotoxic, genotoxic, and polymorphism effects on vanilla planifolia jacks ex andrews after long-term exposure to argovit(®) silver nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215222/
https://www.ncbi.nlm.nih.gov/pubmed/30257465
http://dx.doi.org/10.3390/nano8100754
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