Cargando…

Anatomical Distribution of Diarrhetic Shellfish Toxins (DSTs) in the Japanese Scallop Patinopecten yessoensis and Individual Variability in Scallops and Mytilus edulis Mussels: Statistical Considerations

Diarrhetic shellfish toxins (DSTs) are a group of phycotoxins that include okadaic acid (OA)/dinophysistoxin (DTX) analogues. At present, detailed data on the distribution of DST is insufficient, and studies of the appropriate sample sizes are lacking. This study investigated the DST frequency distr...

Descripción completa

Detalles Bibliográficos
Autores principales: Matsushima, Ryoji, Uchida, Hajime, Watanabe, Ryuichi, Oikawa, Hiroshi, Oogida, Izumi, Kosaka, Yuki, Kanamori, Makoto, Akamine, Tatsuro, Suzuki, Toshiyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215284/
https://www.ncbi.nlm.nih.gov/pubmed/30262768
http://dx.doi.org/10.3390/toxins10100395
_version_ 1783368121493487616
author Matsushima, Ryoji
Uchida, Hajime
Watanabe, Ryuichi
Oikawa, Hiroshi
Oogida, Izumi
Kosaka, Yuki
Kanamori, Makoto
Akamine, Tatsuro
Suzuki, Toshiyuki
author_facet Matsushima, Ryoji
Uchida, Hajime
Watanabe, Ryuichi
Oikawa, Hiroshi
Oogida, Izumi
Kosaka, Yuki
Kanamori, Makoto
Akamine, Tatsuro
Suzuki, Toshiyuki
author_sort Matsushima, Ryoji
collection PubMed
description Diarrhetic shellfish toxins (DSTs) are a group of phycotoxins that include okadaic acid (OA)/dinophysistoxin (DTX) analogues. At present, detailed data on the distribution of DST is insufficient, and studies of the appropriate sample sizes are lacking. This study investigated the DST frequency distribution in scallops and mussels by liquid chromatography-tandem mass spectrometry (LC/MS/MS) and a resampling analysis of existing data was carried out. The DST population-interval and the necessary sample size were also estimated. DSTs are localized in the scallop digestive-gland, and the DST concentrations in scallops were water-depth-dependent. DST concentrations in scallops and mussels showed normal distributions, but mussels tended to contain more DSTs than scallops. In the statistical resampling analysis of the acquired data on scallops and mussels, especially that using the bootstrap method, sample size was difficult to estimate when the DST variation was large. Although the DST population-interval could be statistically estimated from the sample standard deviation of three samples, the sample size corresponded to the risk management level, and the use of 13 or more samples was preferable. The statistical methods used here to analyze individual contents and estimate population content-intervals could be applied in various situations and for shellfish toxins other than DSTs.
format Online
Article
Text
id pubmed-6215284
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62152842018-11-13 Anatomical Distribution of Diarrhetic Shellfish Toxins (DSTs) in the Japanese Scallop Patinopecten yessoensis and Individual Variability in Scallops and Mytilus edulis Mussels: Statistical Considerations Matsushima, Ryoji Uchida, Hajime Watanabe, Ryuichi Oikawa, Hiroshi Oogida, Izumi Kosaka, Yuki Kanamori, Makoto Akamine, Tatsuro Suzuki, Toshiyuki Toxins (Basel) Article Diarrhetic shellfish toxins (DSTs) are a group of phycotoxins that include okadaic acid (OA)/dinophysistoxin (DTX) analogues. At present, detailed data on the distribution of DST is insufficient, and studies of the appropriate sample sizes are lacking. This study investigated the DST frequency distribution in scallops and mussels by liquid chromatography-tandem mass spectrometry (LC/MS/MS) and a resampling analysis of existing data was carried out. The DST population-interval and the necessary sample size were also estimated. DSTs are localized in the scallop digestive-gland, and the DST concentrations in scallops were water-depth-dependent. DST concentrations in scallops and mussels showed normal distributions, but mussels tended to contain more DSTs than scallops. In the statistical resampling analysis of the acquired data on scallops and mussels, especially that using the bootstrap method, sample size was difficult to estimate when the DST variation was large. Although the DST population-interval could be statistically estimated from the sample standard deviation of three samples, the sample size corresponded to the risk management level, and the use of 13 or more samples was preferable. The statistical methods used here to analyze individual contents and estimate population content-intervals could be applied in various situations and for shellfish toxins other than DSTs. MDPI 2018-09-27 /pmc/articles/PMC6215284/ /pubmed/30262768 http://dx.doi.org/10.3390/toxins10100395 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matsushima, Ryoji
Uchida, Hajime
Watanabe, Ryuichi
Oikawa, Hiroshi
Oogida, Izumi
Kosaka, Yuki
Kanamori, Makoto
Akamine, Tatsuro
Suzuki, Toshiyuki
Anatomical Distribution of Diarrhetic Shellfish Toxins (DSTs) in the Japanese Scallop Patinopecten yessoensis and Individual Variability in Scallops and Mytilus edulis Mussels: Statistical Considerations
title Anatomical Distribution of Diarrhetic Shellfish Toxins (DSTs) in the Japanese Scallop Patinopecten yessoensis and Individual Variability in Scallops and Mytilus edulis Mussels: Statistical Considerations
title_full Anatomical Distribution of Diarrhetic Shellfish Toxins (DSTs) in the Japanese Scallop Patinopecten yessoensis and Individual Variability in Scallops and Mytilus edulis Mussels: Statistical Considerations
title_fullStr Anatomical Distribution of Diarrhetic Shellfish Toxins (DSTs) in the Japanese Scallop Patinopecten yessoensis and Individual Variability in Scallops and Mytilus edulis Mussels: Statistical Considerations
title_full_unstemmed Anatomical Distribution of Diarrhetic Shellfish Toxins (DSTs) in the Japanese Scallop Patinopecten yessoensis and Individual Variability in Scallops and Mytilus edulis Mussels: Statistical Considerations
title_short Anatomical Distribution of Diarrhetic Shellfish Toxins (DSTs) in the Japanese Scallop Patinopecten yessoensis and Individual Variability in Scallops and Mytilus edulis Mussels: Statistical Considerations
title_sort anatomical distribution of diarrhetic shellfish toxins (dsts) in the japanese scallop patinopecten yessoensis and individual variability in scallops and mytilus edulis mussels: statistical considerations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215284/
https://www.ncbi.nlm.nih.gov/pubmed/30262768
http://dx.doi.org/10.3390/toxins10100395
work_keys_str_mv AT matsushimaryoji anatomicaldistributionofdiarrheticshellfishtoxinsdstsinthejapanesescalloppatinopectenyessoensisandindividualvariabilityinscallopsandmytilusedulismusselsstatisticalconsiderations
AT uchidahajime anatomicaldistributionofdiarrheticshellfishtoxinsdstsinthejapanesescalloppatinopectenyessoensisandindividualvariabilityinscallopsandmytilusedulismusselsstatisticalconsiderations
AT watanaberyuichi anatomicaldistributionofdiarrheticshellfishtoxinsdstsinthejapanesescalloppatinopectenyessoensisandindividualvariabilityinscallopsandmytilusedulismusselsstatisticalconsiderations
AT oikawahiroshi anatomicaldistributionofdiarrheticshellfishtoxinsdstsinthejapanesescalloppatinopectenyessoensisandindividualvariabilityinscallopsandmytilusedulismusselsstatisticalconsiderations
AT oogidaizumi anatomicaldistributionofdiarrheticshellfishtoxinsdstsinthejapanesescalloppatinopectenyessoensisandindividualvariabilityinscallopsandmytilusedulismusselsstatisticalconsiderations
AT kosakayuki anatomicaldistributionofdiarrheticshellfishtoxinsdstsinthejapanesescalloppatinopectenyessoensisandindividualvariabilityinscallopsandmytilusedulismusselsstatisticalconsiderations
AT kanamorimakoto anatomicaldistributionofdiarrheticshellfishtoxinsdstsinthejapanesescalloppatinopectenyessoensisandindividualvariabilityinscallopsandmytilusedulismusselsstatisticalconsiderations
AT akaminetatsuro anatomicaldistributionofdiarrheticshellfishtoxinsdstsinthejapanesescalloppatinopectenyessoensisandindividualvariabilityinscallopsandmytilusedulismusselsstatisticalconsiderations
AT suzukitoshiyuki anatomicaldistributionofdiarrheticshellfishtoxinsdstsinthejapanesescalloppatinopectenyessoensisandindividualvariabilityinscallopsandmytilusedulismusselsstatisticalconsiderations