Cargando…

Antagonistic Effect of Azoxystrobin Poly (Lactic Acid) Microspheres with Controllable Particle Size on Colletotrichum higginsianum Sacc

Size-controlled azoxystrobin-poly (lactic acid) microspheres (MS) were prepared by an oil/water emulsion solvent evaporation approach. The hydrated mean particle sizes of the MS1, MS2, and MS3 aqueous dispersions were 130.9 nm, 353.4 nm, and 3078.0 nm, respectively. The drug loading and encapsulatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Yao, Junwei, Cui, Bo, Zhao, Xiang, Zhi, Heng, Zeng, Zhanghua, Wang, Yan, Sun, Changjiao, Liu, Guoqiang, Gao, Jinming, Cui, Haixin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215297/
https://www.ncbi.nlm.nih.gov/pubmed/30347772
http://dx.doi.org/10.3390/nano8100857
_version_ 1783368124864659456
author Yao, Junwei
Cui, Bo
Zhao, Xiang
Zhi, Heng
Zeng, Zhanghua
Wang, Yan
Sun, Changjiao
Liu, Guoqiang
Gao, Jinming
Cui, Haixin
author_facet Yao, Junwei
Cui, Bo
Zhao, Xiang
Zhi, Heng
Zeng, Zhanghua
Wang, Yan
Sun, Changjiao
Liu, Guoqiang
Gao, Jinming
Cui, Haixin
author_sort Yao, Junwei
collection PubMed
description Size-controlled azoxystrobin-poly (lactic acid) microspheres (MS) were prepared by an oil/water emulsion solvent evaporation approach. The hydrated mean particle sizes of the MS1, MS2, and MS3 aqueous dispersions were 130.9 nm, 353.4 nm, and 3078.0 nm, respectively. The drug loading and encapsulation efficiency of the azoxystrobin microspheres had a positive relationship with particle size. However, the release rate and percentage of cumulative release were inversely related to particle size. The smaller-sized microspheres had a greater potential to access the target mitochondria. As a result, the more severe oxidative damage of Colletotrichum higginsianum Sacc and higher antagonistic activity were induced by the smaller particle size of azoxystrobin microspheres. The 50% lethal concentrations against Colletotrichum higginsianum Sacc of MS1, MS2, and MS3 were 2.0386 μg/mL, 12.7246 μg/mL, and 21.2905 μg/mL, respectively. These findings reveal that particle size is a critical factor in increasing the bioavailability of insoluble fungicide.
format Online
Article
Text
id pubmed-6215297
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62152972018-11-14 Antagonistic Effect of Azoxystrobin Poly (Lactic Acid) Microspheres with Controllable Particle Size on Colletotrichum higginsianum Sacc Yao, Junwei Cui, Bo Zhao, Xiang Zhi, Heng Zeng, Zhanghua Wang, Yan Sun, Changjiao Liu, Guoqiang Gao, Jinming Cui, Haixin Nanomaterials (Basel) Article Size-controlled azoxystrobin-poly (lactic acid) microspheres (MS) were prepared by an oil/water emulsion solvent evaporation approach. The hydrated mean particle sizes of the MS1, MS2, and MS3 aqueous dispersions were 130.9 nm, 353.4 nm, and 3078.0 nm, respectively. The drug loading and encapsulation efficiency of the azoxystrobin microspheres had a positive relationship with particle size. However, the release rate and percentage of cumulative release were inversely related to particle size. The smaller-sized microspheres had a greater potential to access the target mitochondria. As a result, the more severe oxidative damage of Colletotrichum higginsianum Sacc and higher antagonistic activity were induced by the smaller particle size of azoxystrobin microspheres. The 50% lethal concentrations against Colletotrichum higginsianum Sacc of MS1, MS2, and MS3 were 2.0386 μg/mL, 12.7246 μg/mL, and 21.2905 μg/mL, respectively. These findings reveal that particle size is a critical factor in increasing the bioavailability of insoluble fungicide. MDPI 2018-10-19 /pmc/articles/PMC6215297/ /pubmed/30347772 http://dx.doi.org/10.3390/nano8100857 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yao, Junwei
Cui, Bo
Zhao, Xiang
Zhi, Heng
Zeng, Zhanghua
Wang, Yan
Sun, Changjiao
Liu, Guoqiang
Gao, Jinming
Cui, Haixin
Antagonistic Effect of Azoxystrobin Poly (Lactic Acid) Microspheres with Controllable Particle Size on Colletotrichum higginsianum Sacc
title Antagonistic Effect of Azoxystrobin Poly (Lactic Acid) Microspheres with Controllable Particle Size on Colletotrichum higginsianum Sacc
title_full Antagonistic Effect of Azoxystrobin Poly (Lactic Acid) Microspheres with Controllable Particle Size on Colletotrichum higginsianum Sacc
title_fullStr Antagonistic Effect of Azoxystrobin Poly (Lactic Acid) Microspheres with Controllable Particle Size on Colletotrichum higginsianum Sacc
title_full_unstemmed Antagonistic Effect of Azoxystrobin Poly (Lactic Acid) Microspheres with Controllable Particle Size on Colletotrichum higginsianum Sacc
title_short Antagonistic Effect of Azoxystrobin Poly (Lactic Acid) Microspheres with Controllable Particle Size on Colletotrichum higginsianum Sacc
title_sort antagonistic effect of azoxystrobin poly (lactic acid) microspheres with controllable particle size on colletotrichum higginsianum sacc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215297/
https://www.ncbi.nlm.nih.gov/pubmed/30347772
http://dx.doi.org/10.3390/nano8100857
work_keys_str_mv AT yaojunwei antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc
AT cuibo antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc
AT zhaoxiang antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc
AT zhiheng antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc
AT zengzhanghua antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc
AT wangyan antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc
AT sunchangjiao antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc
AT liuguoqiang antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc
AT gaojinming antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc
AT cuihaixin antagonisticeffectofazoxystrobinpolylacticacidmicrosphereswithcontrollableparticlesizeoncolletotrichumhigginsianumsacc