CRISPR/Cas9-mediated homology-directed repair by ssODNs in zebrafish induces complex mutational patterns resulting from genomic integration of repair-template fragments

Targeted genome editing by CRISPR/Cas9 is extremely well fitted to generate gene disruptions, although precise sequence replacement by CRISPR/Cas9-mediated homology-directed repair (HDR) suffers from low efficiency, impeding its use for high-throughput knock-in disease modeling. In this study, we us...

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Detalles Bibliográficos
Autores principales: Boel, Annekatrien, De Saffel, Hanna, Steyaert, Wouter, Callewaert, Bert, De Paepe, Anne, Coucke, Paul J., Willaert, Andy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215429/
https://www.ncbi.nlm.nih.gov/pubmed/30355591
http://dx.doi.org/10.1242/dmm.035352

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215429/
https://www.ncbi.nlm.nih.gov/pubmed/30355591
http://dx.doi.org/10.1242/dmm.035352

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