Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa

Pseudomonas aeruginosa (Pae) is an opportunistic human pathogen, able to resist host defense mechanisms and antibiotic treatment. In Pae, the master regulator of stress responses RpoS (σ(S)) is involved in the regulation of quorum sensing and several virulence genes. Here, we report that the sRNA Re...

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Autores principales: Thi Bach Nguyen, Hue, Romero A., David, Amman, Fabian, Sorger-Domenigg, Theresa, Tata, Muralidhar, Sonnleitner, Elisabeth, Bläsi, Udo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215814/
https://www.ncbi.nlm.nih.gov/pubmed/30420839
http://dx.doi.org/10.3389/fmicb.2018.02488
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author Thi Bach Nguyen, Hue
Romero A., David
Amman, Fabian
Sorger-Domenigg, Theresa
Tata, Muralidhar
Sonnleitner, Elisabeth
Bläsi, Udo
author_facet Thi Bach Nguyen, Hue
Romero A., David
Amman, Fabian
Sorger-Domenigg, Theresa
Tata, Muralidhar
Sonnleitner, Elisabeth
Bläsi, Udo
author_sort Thi Bach Nguyen, Hue
collection PubMed
description Pseudomonas aeruginosa (Pae) is an opportunistic human pathogen, able to resist host defense mechanisms and antibiotic treatment. In Pae, the master regulator of stress responses RpoS (σ(S)) is involved in the regulation of quorum sensing and several virulence genes. Here, we report that the sRNA ReaL translationally silences rpoS mRNA, which results in a decrease of the RpoS levels. Our studies indicated that ReaL base-pairs with the Shine-Dalgarno region of rpoS mRNA. These studies are underlined by a highly similar transcription profile of a rpoS deletion mutant and a reaL over-expressing strain.
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spelling pubmed-62158142018-11-12 Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa Thi Bach Nguyen, Hue Romero A., David Amman, Fabian Sorger-Domenigg, Theresa Tata, Muralidhar Sonnleitner, Elisabeth Bläsi, Udo Front Microbiol Microbiology Pseudomonas aeruginosa (Pae) is an opportunistic human pathogen, able to resist host defense mechanisms and antibiotic treatment. In Pae, the master regulator of stress responses RpoS (σ(S)) is involved in the regulation of quorum sensing and several virulence genes. Here, we report that the sRNA ReaL translationally silences rpoS mRNA, which results in a decrease of the RpoS levels. Our studies indicated that ReaL base-pairs with the Shine-Dalgarno region of rpoS mRNA. These studies are underlined by a highly similar transcription profile of a rpoS deletion mutant and a reaL over-expressing strain. Frontiers Media S.A. 2018-10-29 /pmc/articles/PMC6215814/ /pubmed/30420839 http://dx.doi.org/10.3389/fmicb.2018.02488 Text en Copyright © 2018 Thi Bach Nguyen, Romero A, Amman, Sorger-Domenigg, Tata, Sonnleitner and Bläsi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Thi Bach Nguyen, Hue
Romero A., David
Amman, Fabian
Sorger-Domenigg, Theresa
Tata, Muralidhar
Sonnleitner, Elisabeth
Bläsi, Udo
Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa
title Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa
title_full Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa
title_fullStr Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa
title_full_unstemmed Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa
title_short Negative Control of RpoS Synthesis by the sRNA ReaL in Pseudomonas aeruginosa
title_sort negative control of rpos synthesis by the srna real in pseudomonas aeruginosa
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215814/
https://www.ncbi.nlm.nih.gov/pubmed/30420839
http://dx.doi.org/10.3389/fmicb.2018.02488
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