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Combining Breath Figures and Supercritical Fluids To Obtain Porous Polymer Scaffolds
[Image: see text] Supercritical fluids technology is a clean methodology to foam polymeric materials. However, this technique provides only the formation of inner porosity, whereas the so-called skin layer is commonly observed at the polymer surface. This article describes a new method for the prepa...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217531/ https://www.ncbi.nlm.nih.gov/pubmed/30411012 http://dx.doi.org/10.1021/acsomega.8b02024 |
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author | Castaño, Marta Martinez-Campos, Enrique Pintado-Sierra, Mercedes García, Carolina Reinecke, Helmut Gallardo, Alberto Rodriguez-Hernandez, Juan Elvira, Carlos |
author_facet | Castaño, Marta Martinez-Campos, Enrique Pintado-Sierra, Mercedes García, Carolina Reinecke, Helmut Gallardo, Alberto Rodriguez-Hernandez, Juan Elvira, Carlos |
author_sort | Castaño, Marta |
collection | PubMed |
description | [Image: see text] Supercritical fluids technology is a clean methodology to foam polymeric materials. However, this technique provides only the formation of inner porosity, whereas the so-called skin layer is commonly observed at the polymer surface. This article describes a new method for the preparation of outer and inner porous poly(ε-caprolactone) (PCL) scaffolds by combination of supercritical CO(2) (SCCO(2)) foaming and the breath figures technique. In the first step, experiments with a SCCO(2) reactor were performed at 35–45 °C, 100–250 bar, and 1–20 min depressurization time. The effect of these parameters in the formation of inner porosity was investigated for an adequate optimization. In a late stage, to provide also surface porosity to the polymeric samples and remove the skin layer, the breath figures technique was employed. The evaluation of porosity was determined by scanning electronic microscopy, mercury porosimetry, and micro X-ray computerized tomography scanning processing the images obtained with the ImageJ software. The results of this study using these two complementary techniques showed the existence of interconnectivity between inner and outer porosity of the samples. Furthermore, thermal transitions and crystallinity of the PCL samples have been analyzed by differential scanning calorimetry. Finally, a preliminary biological evaluation of the resulting scaffolds with mouse endothelial cells (C166-GFP) was performed to assess their biocompatibility and cellular viability. |
format | Online Article Text |
id | pubmed-6217531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-62175312018-11-06 Combining Breath Figures and Supercritical Fluids To Obtain Porous Polymer Scaffolds Castaño, Marta Martinez-Campos, Enrique Pintado-Sierra, Mercedes García, Carolina Reinecke, Helmut Gallardo, Alberto Rodriguez-Hernandez, Juan Elvira, Carlos ACS Omega [Image: see text] Supercritical fluids technology is a clean methodology to foam polymeric materials. However, this technique provides only the formation of inner porosity, whereas the so-called skin layer is commonly observed at the polymer surface. This article describes a new method for the preparation of outer and inner porous poly(ε-caprolactone) (PCL) scaffolds by combination of supercritical CO(2) (SCCO(2)) foaming and the breath figures technique. In the first step, experiments with a SCCO(2) reactor were performed at 35–45 °C, 100–250 bar, and 1–20 min depressurization time. The effect of these parameters in the formation of inner porosity was investigated for an adequate optimization. In a late stage, to provide also surface porosity to the polymeric samples and remove the skin layer, the breath figures technique was employed. The evaluation of porosity was determined by scanning electronic microscopy, mercury porosimetry, and micro X-ray computerized tomography scanning processing the images obtained with the ImageJ software. The results of this study using these two complementary techniques showed the existence of interconnectivity between inner and outer porosity of the samples. Furthermore, thermal transitions and crystallinity of the PCL samples have been analyzed by differential scanning calorimetry. Finally, a preliminary biological evaluation of the resulting scaffolds with mouse endothelial cells (C166-GFP) was performed to assess their biocompatibility and cellular viability. American Chemical Society 2018-10-04 /pmc/articles/PMC6217531/ /pubmed/30411012 http://dx.doi.org/10.1021/acsomega.8b02024 Text en Copyright © 2018 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Castaño, Marta Martinez-Campos, Enrique Pintado-Sierra, Mercedes García, Carolina Reinecke, Helmut Gallardo, Alberto Rodriguez-Hernandez, Juan Elvira, Carlos Combining Breath Figures and Supercritical Fluids To Obtain Porous Polymer Scaffolds |
title | Combining Breath Figures and Supercritical Fluids
To Obtain Porous Polymer Scaffolds |
title_full | Combining Breath Figures and Supercritical Fluids
To Obtain Porous Polymer Scaffolds |
title_fullStr | Combining Breath Figures and Supercritical Fluids
To Obtain Porous Polymer Scaffolds |
title_full_unstemmed | Combining Breath Figures and Supercritical Fluids
To Obtain Porous Polymer Scaffolds |
title_short | Combining Breath Figures and Supercritical Fluids
To Obtain Porous Polymer Scaffolds |
title_sort | combining breath figures and supercritical fluids
to obtain porous polymer scaffolds |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217531/ https://www.ncbi.nlm.nih.gov/pubmed/30411012 http://dx.doi.org/10.1021/acsomega.8b02024 |
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