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Microbubble-Mediated Enhanced Delivery of Curcumin to Cervical Cancer Cells

[Image: see text] The major bottleneck in the current chemotherapy treatment of cancer is the low bioavailability and high cytotoxicity. Targeted delivery of drug to the cancer cells can reduce the cytotoxicity and increase the bioavailability. In this context, microbubbles are currently being explo...

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Autores principales: Upadhyay, Awaneesh, Yagnik, Bhrugu, Desai, Priti, Dalvi, Sameer V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217580/
https://www.ncbi.nlm.nih.gov/pubmed/30411020
http://dx.doi.org/10.1021/acsomega.8b01737
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author Upadhyay, Awaneesh
Yagnik, Bhrugu
Desai, Priti
Dalvi, Sameer V.
author_facet Upadhyay, Awaneesh
Yagnik, Bhrugu
Desai, Priti
Dalvi, Sameer V.
author_sort Upadhyay, Awaneesh
collection PubMed
description [Image: see text] The major bottleneck in the current chemotherapy treatment of cancer is the low bioavailability and high cytotoxicity. Targeted delivery of drug to the cancer cells can reduce the cytotoxicity and increase the bioavailability. In this context, microbubbles are currently being explored as drug-delivery vehicles to effectively deliver drug to the tumors or cancerous cells. Microbubbles when used along with ultrasound can enhance drug uptake and inhibit the growth of tumor cells. Several potential anticancer molecules exhibit poor water solubility, which limits their use in therapeutic applications. Such poorly water soluble molecules can be coadministered with microbubbles or encapsulated within or loaded on the microbubbles surface, to enhance the effectiveness of these molecules against cancer cells. Curcumin is one of such potential anticancer molecules obtained from the rhizome of herbal spice, turmeric. In this work, curcumin-loaded protein microbubbles were synthesized and examined for effective in vitro delivery of curcumin to HeLa cells. Microbubbles in the size range of 1–10 μm were produced using perfluorobutane as core gas and bovine serum albumin (BSA) as shell material and were loaded with curcumin. The amount of curcumin loaded on the microbubble surface was estimated using UV–vis spectroscopy, and the average curcumin loading was found to be ∼54 μM/10(8) microbubbles. Kinetics of in vitro curcumin release from microbubble surface was also estimated, where a 4-fold increase in the rate of curcumin release was obtained in the presence of ultrasound. Sonication and incubation of HeLa cells with curcumin-loaded BSA microbubbles enhanced the uptake of curcumin by ∼250 times. Further, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay confirmed ∼71% decrease in cell viability when HeLa cells were sonicated with curcumin-loaded microbubbles and incubated for 48 h.
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spelling pubmed-62175802018-11-06 Microbubble-Mediated Enhanced Delivery of Curcumin to Cervical Cancer Cells Upadhyay, Awaneesh Yagnik, Bhrugu Desai, Priti Dalvi, Sameer V. ACS Omega [Image: see text] The major bottleneck in the current chemotherapy treatment of cancer is the low bioavailability and high cytotoxicity. Targeted delivery of drug to the cancer cells can reduce the cytotoxicity and increase the bioavailability. In this context, microbubbles are currently being explored as drug-delivery vehicles to effectively deliver drug to the tumors or cancerous cells. Microbubbles when used along with ultrasound can enhance drug uptake and inhibit the growth of tumor cells. Several potential anticancer molecules exhibit poor water solubility, which limits their use in therapeutic applications. Such poorly water soluble molecules can be coadministered with microbubbles or encapsulated within or loaded on the microbubbles surface, to enhance the effectiveness of these molecules against cancer cells. Curcumin is one of such potential anticancer molecules obtained from the rhizome of herbal spice, turmeric. In this work, curcumin-loaded protein microbubbles were synthesized and examined for effective in vitro delivery of curcumin to HeLa cells. Microbubbles in the size range of 1–10 μm were produced using perfluorobutane as core gas and bovine serum albumin (BSA) as shell material and were loaded with curcumin. The amount of curcumin loaded on the microbubble surface was estimated using UV–vis spectroscopy, and the average curcumin loading was found to be ∼54 μM/10(8) microbubbles. Kinetics of in vitro curcumin release from microbubble surface was also estimated, where a 4-fold increase in the rate of curcumin release was obtained in the presence of ultrasound. Sonication and incubation of HeLa cells with curcumin-loaded BSA microbubbles enhanced the uptake of curcumin by ∼250 times. Further, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay confirmed ∼71% decrease in cell viability when HeLa cells were sonicated with curcumin-loaded microbubbles and incubated for 48 h. American Chemical Society 2018-10-08 /pmc/articles/PMC6217580/ /pubmed/30411020 http://dx.doi.org/10.1021/acsomega.8b01737 Text en Copyright © 2018 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
spellingShingle Upadhyay, Awaneesh
Yagnik, Bhrugu
Desai, Priti
Dalvi, Sameer V.
Microbubble-Mediated Enhanced Delivery of Curcumin to Cervical Cancer Cells
title Microbubble-Mediated Enhanced Delivery of Curcumin to Cervical Cancer Cells
title_full Microbubble-Mediated Enhanced Delivery of Curcumin to Cervical Cancer Cells
title_fullStr Microbubble-Mediated Enhanced Delivery of Curcumin to Cervical Cancer Cells
title_full_unstemmed Microbubble-Mediated Enhanced Delivery of Curcumin to Cervical Cancer Cells
title_short Microbubble-Mediated Enhanced Delivery of Curcumin to Cervical Cancer Cells
title_sort microbubble-mediated enhanced delivery of curcumin to cervical cancer cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217580/
https://www.ncbi.nlm.nih.gov/pubmed/30411020
http://dx.doi.org/10.1021/acsomega.8b01737
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