Biosafety evaluation of Janus Fe(3)O(4)-TiO(2) nanoparticles in Sprague Dawley rats after intravenous injection

INTRODUCTION: Newly synthesized Janus-structured Fe(3)O(4)-TiO(2) nanoparticles (NPs) appear to be a promising candidate for the diagnosis and therapy of cancer. Although the toxicity of individual Fe(3)O(4) or TiO(2) NPs has been studied extensively, the toxicity of Janus Fe(3)O(4)-TiO(2) NPs is no...

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Autores principales: Su, Hong, Song, Xin, Li, Juan, Iqbal, Muhammad Zubair, Kenston, Samuel Selorm Fiati, Li, Zhen, Wu, Aiguo, Ding, Min, Zhao, Jinshun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217909/
https://www.ncbi.nlm.nih.gov/pubmed/30464454
http://dx.doi.org/10.2147/IJN.S167851
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author Su, Hong
Song, Xin
Li, Juan
Iqbal, Muhammad Zubair
Kenston, Samuel Selorm Fiati
Li, Zhen
Wu, Aiguo
Ding, Min
Zhao, Jinshun
author_facet Su, Hong
Song, Xin
Li, Juan
Iqbal, Muhammad Zubair
Kenston, Samuel Selorm Fiati
Li, Zhen
Wu, Aiguo
Ding, Min
Zhao, Jinshun
author_sort Su, Hong
collection PubMed
description INTRODUCTION: Newly synthesized Janus-structured Fe(3)O(4)-TiO(2) nanoparticles (NPs) appear to be a promising candidate for the diagnosis and therapy of cancer. Although the toxicity of individual Fe(3)O(4) or TiO(2) NPs has been studied extensively, the toxicity of Janus Fe(3)O(4)-TiO(2) NPs is not clear. METHODS: In this study, the biosafety of both Janus Fe(3)O(4)-TiO(2) NPs (20–25 nm) and the maternal material TiO(2) NPs (7–10 nm) were evaluated in Sprague Dawley rats after one intravenous injection into the tail vein. Healthy rats were randomly divided into one control group and six experimental groups. Thirty days after treatment, rats were killed, then blood and tissue samples were collected for hematological, biochemical, element-content, histopathological, and Western blot analysis. RESULTS: The results show that only a slight Ti element accumulation in the heart, spleen, and liver could be found in the Janus Fe(3)O(4)-TiO(2) NP groups (P>0.05 compared with control). However, significant Ti element accumulation in the spleen, lungs, and liver was found in the TiO(2) NP-treated rats. Both Fe(3)O(4)-TiO(2) NPs and TiO(2) NPs could induce certain histopathological abnormalities. Western blot analysis showed that both NPs could induce certain apoptotic or inflammatory-related molecular protein upregulation in rat livers. A certain degree of alterations in liver function and electrolyte and lipid parameters was also observed in rats treated with both materials. However, compared to Janus structure Fe(3)O(4)-TiO(2) NP-treated groups, TiO(2) NPs at 30 mg/kg showed more severe adverse effects. CONCLUSION: Our results showed that under a low dose (5 mg/kg), both NP types had no significant toxicity in rats. Janus NPs certainly seem less toxic than TiO(2) NPs in rats at 30 mg/kg. To ensure safe use of these newly developed Janus NPs in cancer diagnosis and therapy, further animal studies are needed to evaluate long-term bioeffects.
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spelling pubmed-62179092018-11-21 Biosafety evaluation of Janus Fe(3)O(4)-TiO(2) nanoparticles in Sprague Dawley rats after intravenous injection Su, Hong Song, Xin Li, Juan Iqbal, Muhammad Zubair Kenston, Samuel Selorm Fiati Li, Zhen Wu, Aiguo Ding, Min Zhao, Jinshun Int J Nanomedicine Original Research INTRODUCTION: Newly synthesized Janus-structured Fe(3)O(4)-TiO(2) nanoparticles (NPs) appear to be a promising candidate for the diagnosis and therapy of cancer. Although the toxicity of individual Fe(3)O(4) or TiO(2) NPs has been studied extensively, the toxicity of Janus Fe(3)O(4)-TiO(2) NPs is not clear. METHODS: In this study, the biosafety of both Janus Fe(3)O(4)-TiO(2) NPs (20–25 nm) and the maternal material TiO(2) NPs (7–10 nm) were evaluated in Sprague Dawley rats after one intravenous injection into the tail vein. Healthy rats were randomly divided into one control group and six experimental groups. Thirty days after treatment, rats were killed, then blood and tissue samples were collected for hematological, biochemical, element-content, histopathological, and Western blot analysis. RESULTS: The results show that only a slight Ti element accumulation in the heart, spleen, and liver could be found in the Janus Fe(3)O(4)-TiO(2) NP groups (P>0.05 compared with control). However, significant Ti element accumulation in the spleen, lungs, and liver was found in the TiO(2) NP-treated rats. Both Fe(3)O(4)-TiO(2) NPs and TiO(2) NPs could induce certain histopathological abnormalities. Western blot analysis showed that both NPs could induce certain apoptotic or inflammatory-related molecular protein upregulation in rat livers. A certain degree of alterations in liver function and electrolyte and lipid parameters was also observed in rats treated with both materials. However, compared to Janus structure Fe(3)O(4)-TiO(2) NP-treated groups, TiO(2) NPs at 30 mg/kg showed more severe adverse effects. CONCLUSION: Our results showed that under a low dose (5 mg/kg), both NP types had no significant toxicity in rats. Janus NPs certainly seem less toxic than TiO(2) NPs in rats at 30 mg/kg. To ensure safe use of these newly developed Janus NPs in cancer diagnosis and therapy, further animal studies are needed to evaluate long-term bioeffects. Dove Medical Press 2018-10-31 /pmc/articles/PMC6217909/ /pubmed/30464454 http://dx.doi.org/10.2147/IJN.S167851 Text en © 2018 Su et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Su, Hong
Song, Xin
Li, Juan
Iqbal, Muhammad Zubair
Kenston, Samuel Selorm Fiati
Li, Zhen
Wu, Aiguo
Ding, Min
Zhao, Jinshun
Biosafety evaluation of Janus Fe(3)O(4)-TiO(2) nanoparticles in Sprague Dawley rats after intravenous injection
title Biosafety evaluation of Janus Fe(3)O(4)-TiO(2) nanoparticles in Sprague Dawley rats after intravenous injection
title_full Biosafety evaluation of Janus Fe(3)O(4)-TiO(2) nanoparticles in Sprague Dawley rats after intravenous injection
title_fullStr Biosafety evaluation of Janus Fe(3)O(4)-TiO(2) nanoparticles in Sprague Dawley rats after intravenous injection
title_full_unstemmed Biosafety evaluation of Janus Fe(3)O(4)-TiO(2) nanoparticles in Sprague Dawley rats after intravenous injection
title_short Biosafety evaluation of Janus Fe(3)O(4)-TiO(2) nanoparticles in Sprague Dawley rats after intravenous injection
title_sort biosafety evaluation of janus fe(3)o(4)-tio(2) nanoparticles in sprague dawley rats after intravenous injection
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6217909/
https://www.ncbi.nlm.nih.gov/pubmed/30464454
http://dx.doi.org/10.2147/IJN.S167851
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