Cargando…

Comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle

We have created a new, fast-growing strain of common carp with higher unsaturated fatty acid content in muscle. To better understand the impacts of gene regulation in intestinal tissue on growth and unsaturated fatty acid content, we conducted a comparative RNA-Seq transcriptome analysis between int...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Chengfeng, Su, Shengyan, Li, Xinyuan, Li, Bing, Yang, Baojuan, Zhu, Jian, Wang, Weimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218049/
https://www.ncbi.nlm.nih.gov/pubmed/30395585
http://dx.doi.org/10.1371/journal.pone.0206615
_version_ 1783368389229543424
author Zhang, Chengfeng
Su, Shengyan
Li, Xinyuan
Li, Bing
Yang, Baojuan
Zhu, Jian
Wang, Weimin
author_facet Zhang, Chengfeng
Su, Shengyan
Li, Xinyuan
Li, Bing
Yang, Baojuan
Zhu, Jian
Wang, Weimin
author_sort Zhang, Chengfeng
collection PubMed
description We have created a new, fast-growing strain of common carp with higher unsaturated fatty acid content in muscle. To better understand the impacts of gene regulation in intestinal tissue on growth and unsaturated fatty acid content, we conducted a comparative RNA-Seq transcriptome analysis between intestine samples of Selected and Control groups (and corroborated selected results by PCR). After eight weeks of cage culture, weight gain of the Selected group was 20.84% higher. In muscles of the control group, monounsaturated fatty acids (FAs) were more abundant, whereas polyunsaturated FAs were more abundant in muscles of the Selected group. In total, we found 106 differentially expressed genes (DEGs) between the two groups. Only the endocytosis pathway was significantly enriched in DEGs, with two upregulated genes: il2rb and ehd1. The latter is involved in the growth hormone/insulin-like growth factor (Gh/Igf) axis, which plays a key role in the regulation of growth in animals. tll2, which is known to be associated with intestinal regeneration, was extremely highly upregulated in both transcriptomic (infinite) and qPCR (610.70) analyses. Two of the upregulated genes are associated with the fatty acid metabolism, several genes are likely to be indicators of heightened transcription levels, several are associated with metabolic and developmental roles, several with neuronal functions (including two with vision), several with the immune system, and two downregulated genes with the development of vasculature. The higher growth rate of the Selected group is likely to be at least partially attributed to increased endocytosis efficiency and genetically-driven behavioural differences (higher aggression levels). There are some indications that this new strain might have slightly impaired immune responses, and a higher propensity for inherited diseases leading to sight impairment, as well for neurodegenerative diseases in general, but these indications still need to be confirmed.
format Online
Article
Text
id pubmed-6218049
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-62180492018-11-19 Comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle Zhang, Chengfeng Su, Shengyan Li, Xinyuan Li, Bing Yang, Baojuan Zhu, Jian Wang, Weimin PLoS One Research Article We have created a new, fast-growing strain of common carp with higher unsaturated fatty acid content in muscle. To better understand the impacts of gene regulation in intestinal tissue on growth and unsaturated fatty acid content, we conducted a comparative RNA-Seq transcriptome analysis between intestine samples of Selected and Control groups (and corroborated selected results by PCR). After eight weeks of cage culture, weight gain of the Selected group was 20.84% higher. In muscles of the control group, monounsaturated fatty acids (FAs) were more abundant, whereas polyunsaturated FAs were more abundant in muscles of the Selected group. In total, we found 106 differentially expressed genes (DEGs) between the two groups. Only the endocytosis pathway was significantly enriched in DEGs, with two upregulated genes: il2rb and ehd1. The latter is involved in the growth hormone/insulin-like growth factor (Gh/Igf) axis, which plays a key role in the regulation of growth in animals. tll2, which is known to be associated with intestinal regeneration, was extremely highly upregulated in both transcriptomic (infinite) and qPCR (610.70) analyses. Two of the upregulated genes are associated with the fatty acid metabolism, several genes are likely to be indicators of heightened transcription levels, several are associated with metabolic and developmental roles, several with neuronal functions (including two with vision), several with the immune system, and two downregulated genes with the development of vasculature. The higher growth rate of the Selected group is likely to be at least partially attributed to increased endocytosis efficiency and genetically-driven behavioural differences (higher aggression levels). There are some indications that this new strain might have slightly impaired immune responses, and a higher propensity for inherited diseases leading to sight impairment, as well for neurodegenerative diseases in general, but these indications still need to be confirmed. Public Library of Science 2018-11-05 /pmc/articles/PMC6218049/ /pubmed/30395585 http://dx.doi.org/10.1371/journal.pone.0206615 Text en © 2018 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Chengfeng
Su, Shengyan
Li, Xinyuan
Li, Bing
Yang, Baojuan
Zhu, Jian
Wang, Weimin
Comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle
title Comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle
title_full Comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle
title_fullStr Comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle
title_full_unstemmed Comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle
title_short Comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle
title_sort comparative transcriptomics identifies genes differentially expressed in the intestine of a new fast-growing strain of common carp with higher unsaturated fatty acid content in muscle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218049/
https://www.ncbi.nlm.nih.gov/pubmed/30395585
http://dx.doi.org/10.1371/journal.pone.0206615
work_keys_str_mv AT zhangchengfeng comparativetranscriptomicsidentifiesgenesdifferentiallyexpressedintheintestineofanewfastgrowingstrainofcommoncarpwithhigherunsaturatedfattyacidcontentinmuscle
AT sushengyan comparativetranscriptomicsidentifiesgenesdifferentiallyexpressedintheintestineofanewfastgrowingstrainofcommoncarpwithhigherunsaturatedfattyacidcontentinmuscle
AT lixinyuan comparativetranscriptomicsidentifiesgenesdifferentiallyexpressedintheintestineofanewfastgrowingstrainofcommoncarpwithhigherunsaturatedfattyacidcontentinmuscle
AT libing comparativetranscriptomicsidentifiesgenesdifferentiallyexpressedintheintestineofanewfastgrowingstrainofcommoncarpwithhigherunsaturatedfattyacidcontentinmuscle
AT yangbaojuan comparativetranscriptomicsidentifiesgenesdifferentiallyexpressedintheintestineofanewfastgrowingstrainofcommoncarpwithhigherunsaturatedfattyacidcontentinmuscle
AT zhujian comparativetranscriptomicsidentifiesgenesdifferentiallyexpressedintheintestineofanewfastgrowingstrainofcommoncarpwithhigherunsaturatedfattyacidcontentinmuscle
AT wangweimin comparativetranscriptomicsidentifiesgenesdifferentiallyexpressedintheintestineofanewfastgrowingstrainofcommoncarpwithhigherunsaturatedfattyacidcontentinmuscle