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Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse)

Recent outbreaks of locally transmitted dengue and Zika viruses in Florida have placed more emphasis on integrated vector management plans for Aedes aegypti (L.) and Aedes albopictus Skuse. Adulticiding, primarily with pyrethroids, is often employed for the immediate control of potentially arbovirus...

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Autores principales: Estep, Alden S., Sanscrainte, Neil D., Waits, Christy M., Bernard, Sarah J., Lloyd, Aaron M., Lucas, Keira J., Buckner, Eva A., Vaidyanathan, Rajeev, Morreale, Rachel, Conti, Lisa A., Becnel, James J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218098/
https://www.ncbi.nlm.nih.gov/pubmed/30356237
http://dx.doi.org/10.1371/journal.pntd.0006544
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author Estep, Alden S.
Sanscrainte, Neil D.
Waits, Christy M.
Bernard, Sarah J.
Lloyd, Aaron M.
Lucas, Keira J.
Buckner, Eva A.
Vaidyanathan, Rajeev
Morreale, Rachel
Conti, Lisa A.
Becnel, James J.
author_facet Estep, Alden S.
Sanscrainte, Neil D.
Waits, Christy M.
Bernard, Sarah J.
Lloyd, Aaron M.
Lucas, Keira J.
Buckner, Eva A.
Vaidyanathan, Rajeev
Morreale, Rachel
Conti, Lisa A.
Becnel, James J.
author_sort Estep, Alden S.
collection PubMed
description Recent outbreaks of locally transmitted dengue and Zika viruses in Florida have placed more emphasis on integrated vector management plans for Aedes aegypti (L.) and Aedes albopictus Skuse. Adulticiding, primarily with pyrethroids, is often employed for the immediate control of potentially arbovirus-infected mosquitoes during outbreak situations. While pyrethroid resistance is common in Ae. aegypti worldwide and testing is recommended by CDC and WHO, resistance to this class of products has not been widely examined or quantified in Florida. To address this information gap, we performed the first study to quantify both pyrethroid resistance and genetic markers of pyrethroid resistance in Ae. aegypti and Ae. albopictus strains in Florida. Using direct topical application to measure intrinsic toxicity, we examined 21 Ae. aegypti strains from 9 counties and found permethrin resistance (resistance ratio (RR) = 6-61-fold) in all strains when compared to the susceptible ORL1952 control strain. Permethrin resistance in five strains of Ae. albopictus was very low (RR<1.6) even when collected from the same containers producing resistant Ae. aegypti. Characterization of two sodium channel kdr alleles associated with pyrethroid-resistance showed widespread distribution in 62 strains of Ae. aegypti. The 1534 phenylalanine to cysteine (F1534C) single nucleotide polymorphism SNP was fixed or nearly fixed in all strains regardless of RR. We observed much more variation in the 1016 valine to isoleucine (V1016I) allele and observed that an increasing frequency of the homozygous V1016I allele correlates strongly with increased RR (Pearson corr = 0.905). In agreement with previous studies, we observed a very low frequency of three kdr genotypes, IIFF, VIFF, and IIFC. In this study, we provide a statewide examination of pyrethroid resistance, and demonstrate that permethrin resistance and the genetic markers for resistance are widely present in FL Ae. aegypti. Resistance testing should be included in an effective management program.
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spelling pubmed-62180982018-11-19 Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse) Estep, Alden S. Sanscrainte, Neil D. Waits, Christy M. Bernard, Sarah J. Lloyd, Aaron M. Lucas, Keira J. Buckner, Eva A. Vaidyanathan, Rajeev Morreale, Rachel Conti, Lisa A. Becnel, James J. PLoS Negl Trop Dis Research Article Recent outbreaks of locally transmitted dengue and Zika viruses in Florida have placed more emphasis on integrated vector management plans for Aedes aegypti (L.) and Aedes albopictus Skuse. Adulticiding, primarily with pyrethroids, is often employed for the immediate control of potentially arbovirus-infected mosquitoes during outbreak situations. While pyrethroid resistance is common in Ae. aegypti worldwide and testing is recommended by CDC and WHO, resistance to this class of products has not been widely examined or quantified in Florida. To address this information gap, we performed the first study to quantify both pyrethroid resistance and genetic markers of pyrethroid resistance in Ae. aegypti and Ae. albopictus strains in Florida. Using direct topical application to measure intrinsic toxicity, we examined 21 Ae. aegypti strains from 9 counties and found permethrin resistance (resistance ratio (RR) = 6-61-fold) in all strains when compared to the susceptible ORL1952 control strain. Permethrin resistance in five strains of Ae. albopictus was very low (RR<1.6) even when collected from the same containers producing resistant Ae. aegypti. Characterization of two sodium channel kdr alleles associated with pyrethroid-resistance showed widespread distribution in 62 strains of Ae. aegypti. The 1534 phenylalanine to cysteine (F1534C) single nucleotide polymorphism SNP was fixed or nearly fixed in all strains regardless of RR. We observed much more variation in the 1016 valine to isoleucine (V1016I) allele and observed that an increasing frequency of the homozygous V1016I allele correlates strongly with increased RR (Pearson corr = 0.905). In agreement with previous studies, we observed a very low frequency of three kdr genotypes, IIFF, VIFF, and IIFC. In this study, we provide a statewide examination of pyrethroid resistance, and demonstrate that permethrin resistance and the genetic markers for resistance are widely present in FL Ae. aegypti. Resistance testing should be included in an effective management program. Public Library of Science 2018-10-24 /pmc/articles/PMC6218098/ /pubmed/30356237 http://dx.doi.org/10.1371/journal.pntd.0006544 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Estep, Alden S.
Sanscrainte, Neil D.
Waits, Christy M.
Bernard, Sarah J.
Lloyd, Aaron M.
Lucas, Keira J.
Buckner, Eva A.
Vaidyanathan, Rajeev
Morreale, Rachel
Conti, Lisa A.
Becnel, James J.
Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse)
title Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse)
title_full Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse)
title_fullStr Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse)
title_full_unstemmed Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse)
title_short Quantification of permethrin resistance and kdr alleles in Florida strains of Aedes aegypti (L.) and Aedes albopictus (Skuse)
title_sort quantification of permethrin resistance and kdr alleles in florida strains of aedes aegypti (l.) and aedes albopictus (skuse)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218098/
https://www.ncbi.nlm.nih.gov/pubmed/30356237
http://dx.doi.org/10.1371/journal.pntd.0006544
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