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Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans

CRISPR-based genome editing using ribonucleoprotein complexes and synthetic single-stranded oligodeoxynucleotide (ssODN) donors can be highly effective. However, reproducibility can vary, and precise, targeted integration of longer constructs—such as green fluorescent protein tags remains challengin...

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Detalles Bibliográficos
Autores principales:	Dokshin, Gregoriy A., Ghanta, Krishna S., Piscopo, Katherine M., Mello, Craig C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Genetics Society of America 2018
Materias:	Investigations
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218216/ https://www.ncbi.nlm.nih.gov/pubmed/30213854 http://dx.doi.org/10.1534/genetics.118.301532

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