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High Sensitive and Non-invasive ctDNAs Sequencing Facilitate Clinical Diagnosis And Clinical Guidance of Non-small Cell Lung Cancer Patient: A Time Course Study

Lung cancer is one of leading causes of cancer death all over the world. Non-small cell lung cancer (NSCLC) is the most predominant subtype of lung cancer. Molecular targeting therapy has been shown great success in the treatment of advanced NSCLC. Thus, an easy, sensitive, and specific way of recog...

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Autores principales: Li, Yongqiang, Lv, Jiajia, Wan, Shaogui, Xin, Junfang, Xie, Tiantian, Li, Tao, Zhu, Wan, Zhang, Guosen, Wang, Yunlong, Tang, Yitai, Li, Ao, Guo, Xiangqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218404/
https://www.ncbi.nlm.nih.gov/pubmed/30425968
http://dx.doi.org/10.3389/fonc.2018.00491
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author Li, Yongqiang
Lv, Jiajia
Wan, Shaogui
Xin, Junfang
Xie, Tiantian
Li, Tao
Zhu, Wan
Zhang, Guosen
Wang, Yunlong
Tang, Yitai
Li, Ao
Guo, Xiangqian
author_facet Li, Yongqiang
Lv, Jiajia
Wan, Shaogui
Xin, Junfang
Xie, Tiantian
Li, Tao
Zhu, Wan
Zhang, Guosen
Wang, Yunlong
Tang, Yitai
Li, Ao
Guo, Xiangqian
author_sort Li, Yongqiang
collection PubMed
description Lung cancer is one of leading causes of cancer death all over the world. Non-small cell lung cancer (NSCLC) is the most predominant subtype of lung cancer. Molecular targeting therapy has been shown great success in the treatment of advanced NSCLC. Thus, an easy, sensitive, and specific way of recognizing therapeutic gene targets would help to select effective treatments, to improve physical condition and increase patient survival. In this study, we recruited and followed up a female NSCLC patient, whose plasma ctDNAs (circulating tumor DNAs), blood cell DNAs, psDNAs (pleural effusion supernatant DNAs), and ppDNAs (pleural effusion pellet DNAs), were collected and analyzed over periodic time points by methods of next generation sequencing (NGS), droplet digital PCR (ddPCR), and Amplification Refractory Mutation System (ARMS). In addition, pleural effusion pellets were stained by IHC (immunohistochemistry). The investigation results showed that EGFR L858R mutation was recognized by methods of NGS, ddPCR, and ARMS, while EGFR T790M mutation was only identified by methods of NGS and ddPCR but not ARMS, indicating that ARMS as an auxiliary clinical diagnostic method, is less sensitive and less reliable than NGS and ddPCR. In summary, the non-invasive and sensitive way of collecting ctDNAs for NGS and/or ddPCR screenings offers patients new diagnosis and therapeutic options.
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spelling pubmed-62184042018-11-13 High Sensitive and Non-invasive ctDNAs Sequencing Facilitate Clinical Diagnosis And Clinical Guidance of Non-small Cell Lung Cancer Patient: A Time Course Study Li, Yongqiang Lv, Jiajia Wan, Shaogui Xin, Junfang Xie, Tiantian Li, Tao Zhu, Wan Zhang, Guosen Wang, Yunlong Tang, Yitai Li, Ao Guo, Xiangqian Front Oncol Oncology Lung cancer is one of leading causes of cancer death all over the world. Non-small cell lung cancer (NSCLC) is the most predominant subtype of lung cancer. Molecular targeting therapy has been shown great success in the treatment of advanced NSCLC. Thus, an easy, sensitive, and specific way of recognizing therapeutic gene targets would help to select effective treatments, to improve physical condition and increase patient survival. In this study, we recruited and followed up a female NSCLC patient, whose plasma ctDNAs (circulating tumor DNAs), blood cell DNAs, psDNAs (pleural effusion supernatant DNAs), and ppDNAs (pleural effusion pellet DNAs), were collected and analyzed over periodic time points by methods of next generation sequencing (NGS), droplet digital PCR (ddPCR), and Amplification Refractory Mutation System (ARMS). In addition, pleural effusion pellets were stained by IHC (immunohistochemistry). The investigation results showed that EGFR L858R mutation was recognized by methods of NGS, ddPCR, and ARMS, while EGFR T790M mutation was only identified by methods of NGS and ddPCR but not ARMS, indicating that ARMS as an auxiliary clinical diagnostic method, is less sensitive and less reliable than NGS and ddPCR. In summary, the non-invasive and sensitive way of collecting ctDNAs for NGS and/or ddPCR screenings offers patients new diagnosis and therapeutic options. Frontiers Media S.A. 2018-10-30 /pmc/articles/PMC6218404/ /pubmed/30425968 http://dx.doi.org/10.3389/fonc.2018.00491 Text en Copyright © 2018 Li, Lv, Wan, Xin, Xie, Li, Zhu, Zhang, Wang, Tang, Li and Guo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Li, Yongqiang
Lv, Jiajia
Wan, Shaogui
Xin, Junfang
Xie, Tiantian
Li, Tao
Zhu, Wan
Zhang, Guosen
Wang, Yunlong
Tang, Yitai
Li, Ao
Guo, Xiangqian
High Sensitive and Non-invasive ctDNAs Sequencing Facilitate Clinical Diagnosis And Clinical Guidance of Non-small Cell Lung Cancer Patient: A Time Course Study
title High Sensitive and Non-invasive ctDNAs Sequencing Facilitate Clinical Diagnosis And Clinical Guidance of Non-small Cell Lung Cancer Patient: A Time Course Study
title_full High Sensitive and Non-invasive ctDNAs Sequencing Facilitate Clinical Diagnosis And Clinical Guidance of Non-small Cell Lung Cancer Patient: A Time Course Study
title_fullStr High Sensitive and Non-invasive ctDNAs Sequencing Facilitate Clinical Diagnosis And Clinical Guidance of Non-small Cell Lung Cancer Patient: A Time Course Study
title_full_unstemmed High Sensitive and Non-invasive ctDNAs Sequencing Facilitate Clinical Diagnosis And Clinical Guidance of Non-small Cell Lung Cancer Patient: A Time Course Study
title_short High Sensitive and Non-invasive ctDNAs Sequencing Facilitate Clinical Diagnosis And Clinical Guidance of Non-small Cell Lung Cancer Patient: A Time Course Study
title_sort high sensitive and non-invasive ctdnas sequencing facilitate clinical diagnosis and clinical guidance of non-small cell lung cancer patient: a time course study
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218404/
https://www.ncbi.nlm.nih.gov/pubmed/30425968
http://dx.doi.org/10.3389/fonc.2018.00491
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