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MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management

Negative Pressure Wound Therapy (NPWT), a widely used modality in the management of surgical and trauma wounds, offers clear benefits over conventional wound healing strategies. Despite the wide-ranging effects ascribed to NPWT, the precise molecular mechanisms underlying the accelerated healing sup...

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Autores principales: Bellot, Gregory Lucien, Dong, Xiaoke, Lahiri, Amitabha, Sebastin, Sandeep Jacob, Batinic-Haberle, Ines, Pervaiz, Shazib, Puhaindran, Mark Edward
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218638/
https://www.ncbi.nlm.nih.gov/pubmed/30390545
http://dx.doi.org/10.1016/j.redox.2018.10.014
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author Bellot, Gregory Lucien
Dong, Xiaoke
Lahiri, Amitabha
Sebastin, Sandeep Jacob
Batinic-Haberle, Ines
Pervaiz, Shazib
Puhaindran, Mark Edward
author_facet Bellot, Gregory Lucien
Dong, Xiaoke
Lahiri, Amitabha
Sebastin, Sandeep Jacob
Batinic-Haberle, Ines
Pervaiz, Shazib
Puhaindran, Mark Edward
author_sort Bellot, Gregory Lucien
collection PubMed
description Negative Pressure Wound Therapy (NPWT), a widely used modality in the management of surgical and trauma wounds, offers clear benefits over conventional wound healing strategies. Despite the wide-ranging effects ascribed to NPWT, the precise molecular mechanisms underlying the accelerated healing supported by NPWT remains poorly understood. Notably, cellular redox status-a product of the balance between cellular reactive oxygen species (ROS) production and anti-oxidant defense systems-plays an important role in wound healing and dysregulation of redox homeostasis has a profound effect on wound healing. Here we investigated potential links between the use of NPWT and the regulation of antioxidant mechanisms. Using patient samples and a rodent model of acute injury, we observed a significant accumulation of MnSOD protein as well as higher enzymatic activity in tissues upon NPWT. As a proof of concept and to outline the important role of SOD activity in wound healing, we replaced NPWT by the topical application of a MnSOD mimetic, Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin (MnTE-2-PyP(5+), MnE, BMX-010, AEOl10113) in the rodent model. We observed that MnE is a potent wound healing enhancer as it appears to facilitate the formation of new tissue within the wound bed and consequently advances wound closure by two days, compared to the non-treated animals. Taken together, these results show for the first time a link between NPWT and regulation of antioxidant mechanism through the maintenance of MnSOD activity. Additionally this discovery outlined the potential role of MnSOD mimetics as topical agents enhancing wound healing.
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spelling pubmed-62186382018-11-19 MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management Bellot, Gregory Lucien Dong, Xiaoke Lahiri, Amitabha Sebastin, Sandeep Jacob Batinic-Haberle, Ines Pervaiz, Shazib Puhaindran, Mark Edward Redox Biol Research Paper Negative Pressure Wound Therapy (NPWT), a widely used modality in the management of surgical and trauma wounds, offers clear benefits over conventional wound healing strategies. Despite the wide-ranging effects ascribed to NPWT, the precise molecular mechanisms underlying the accelerated healing supported by NPWT remains poorly understood. Notably, cellular redox status-a product of the balance between cellular reactive oxygen species (ROS) production and anti-oxidant defense systems-plays an important role in wound healing and dysregulation of redox homeostasis has a profound effect on wound healing. Here we investigated potential links between the use of NPWT and the regulation of antioxidant mechanisms. Using patient samples and a rodent model of acute injury, we observed a significant accumulation of MnSOD protein as well as higher enzymatic activity in tissues upon NPWT. As a proof of concept and to outline the important role of SOD activity in wound healing, we replaced NPWT by the topical application of a MnSOD mimetic, Mn(III) meso-tetrakis(N-ethylpyridinium-2-yl)porphyrin (MnTE-2-PyP(5+), MnE, BMX-010, AEOl10113) in the rodent model. We observed that MnE is a potent wound healing enhancer as it appears to facilitate the formation of new tissue within the wound bed and consequently advances wound closure by two days, compared to the non-treated animals. Taken together, these results show for the first time a link between NPWT and regulation of antioxidant mechanism through the maintenance of MnSOD activity. Additionally this discovery outlined the potential role of MnSOD mimetics as topical agents enhancing wound healing. Elsevier 2018-10-23 /pmc/articles/PMC6218638/ /pubmed/30390545 http://dx.doi.org/10.1016/j.redox.2018.10.014 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Bellot, Gregory Lucien
Dong, Xiaoke
Lahiri, Amitabha
Sebastin, Sandeep Jacob
Batinic-Haberle, Ines
Pervaiz, Shazib
Puhaindran, Mark Edward
MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management
title MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management
title_full MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management
title_fullStr MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management
title_full_unstemmed MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management
title_short MnSOD is implicated in accelerated wound healing upon Negative Pressure Wound Therapy (NPWT): A case in point for MnSOD mimetics as adjuvants for wound management
title_sort mnsod is implicated in accelerated wound healing upon negative pressure wound therapy (npwt): a case in point for mnsod mimetics as adjuvants for wound management
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218638/
https://www.ncbi.nlm.nih.gov/pubmed/30390545
http://dx.doi.org/10.1016/j.redox.2018.10.014
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