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Ethanol Enhances Estrogen Mediated Angiogenesis in Breast Cancer
Angiogenesis, a highly regulated process, is exploited by tumors like breast cancer to ensure a constant supply of oxygen and nutrients and is key for tumor survival and progression. Estrogen and alcohol independently have been observed to contribute to angiogenesis in breast cancer but their combin...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218769/ https://www.ncbi.nlm.nih.gov/pubmed/30410590 http://dx.doi.org/10.7150/jca.25581 |
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author | Maniyar, Rachana Chakraborty, Sanjukta Suriano, Robert |
author_facet | Maniyar, Rachana Chakraborty, Sanjukta Suriano, Robert |
author_sort | Maniyar, Rachana |
collection | PubMed |
description | Angiogenesis, a highly regulated process, is exploited by tumors like breast cancer to ensure a constant supply of oxygen and nutrients and is key for tumor survival and progression. Estrogen and alcohol independently have been observed to contribute to angiogenesis in breast cancer but their combinatorial effects have never been evaluated. The exact mechanism by which estrogen and alcohol contribute to breast cancer angiogenesis remains to be elucidated. In this study, we defined the in vitro effects of the combination of estrogen and alcohol in breast cancer angiogenesis using the tubulogenesis and scratch wound assays. Conditioned media, generated by culturing the murine mammary cancer cell line, TG1-1, in estrogen and ethanol, enhanced tubule formation and migration as well as modulated the MAP Kinase pathway in the murine endothelial cell line, SVEC4-10. Additionally, estrogen and ethanol in combination enhanced the expression of the pro-angiogenic factors VEGF, MMP-9, and eNOS, and modulated Akt activation. These observations suggest that TG1-1 cells secrete pro-angiogenic molecules in response to the combination of estrogen and ethanol that modulate the morphological and migratory properties of endothelial cells. The data presented in this study, is the first in attempting to link the cooperative activity between estrogen and ethanol in breast cancer progression, underscoring correlations first made by epidemiological observations linking the two. |
format | Online Article Text |
id | pubmed-6218769 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-62187692018-11-08 Ethanol Enhances Estrogen Mediated Angiogenesis in Breast Cancer Maniyar, Rachana Chakraborty, Sanjukta Suriano, Robert J Cancer Research Paper Angiogenesis, a highly regulated process, is exploited by tumors like breast cancer to ensure a constant supply of oxygen and nutrients and is key for tumor survival and progression. Estrogen and alcohol independently have been observed to contribute to angiogenesis in breast cancer but their combinatorial effects have never been evaluated. The exact mechanism by which estrogen and alcohol contribute to breast cancer angiogenesis remains to be elucidated. In this study, we defined the in vitro effects of the combination of estrogen and alcohol in breast cancer angiogenesis using the tubulogenesis and scratch wound assays. Conditioned media, generated by culturing the murine mammary cancer cell line, TG1-1, in estrogen and ethanol, enhanced tubule formation and migration as well as modulated the MAP Kinase pathway in the murine endothelial cell line, SVEC4-10. Additionally, estrogen and ethanol in combination enhanced the expression of the pro-angiogenic factors VEGF, MMP-9, and eNOS, and modulated Akt activation. These observations suggest that TG1-1 cells secrete pro-angiogenic molecules in response to the combination of estrogen and ethanol that modulate the morphological and migratory properties of endothelial cells. The data presented in this study, is the first in attempting to link the cooperative activity between estrogen and ethanol in breast cancer progression, underscoring correlations first made by epidemiological observations linking the two. Ivyspring International Publisher 2018-10-06 /pmc/articles/PMC6218769/ /pubmed/30410590 http://dx.doi.org/10.7150/jca.25581 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Maniyar, Rachana Chakraborty, Sanjukta Suriano, Robert Ethanol Enhances Estrogen Mediated Angiogenesis in Breast Cancer |
title | Ethanol Enhances Estrogen Mediated Angiogenesis in Breast Cancer |
title_full | Ethanol Enhances Estrogen Mediated Angiogenesis in Breast Cancer |
title_fullStr | Ethanol Enhances Estrogen Mediated Angiogenesis in Breast Cancer |
title_full_unstemmed | Ethanol Enhances Estrogen Mediated Angiogenesis in Breast Cancer |
title_short | Ethanol Enhances Estrogen Mediated Angiogenesis in Breast Cancer |
title_sort | ethanol enhances estrogen mediated angiogenesis in breast cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218769/ https://www.ncbi.nlm.nih.gov/pubmed/30410590 http://dx.doi.org/10.7150/jca.25581 |
work_keys_str_mv | AT maniyarrachana ethanolenhancesestrogenmediatedangiogenesisinbreastcancer AT chakrabortysanjukta ethanolenhancesestrogenmediatedangiogenesisinbreastcancer AT surianorobert ethanolenhancesestrogenmediatedangiogenesisinbreastcancer |