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Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice

BACKGROUND: In this work, we report an electrochemical biosensor for the detection of anti-hemagglutinin antibodies against the swine virus H1N1 present in mice sera immunized with mixture of His(6)-H1 HA in monomeric and oligomeric form. The oriented immobilization of the recombinant His-tagged hem...

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Autores principales: Mikuła, Edyta, Silva, Cristiane Erdmann, Kopera, Edyta, Zdanowski, Konrad, Radecki, Jerzy, Radecka, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218974/
https://www.ncbi.nlm.nih.gov/pubmed/30400888
http://dx.doi.org/10.1186/s12917-018-1668-9
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author Mikuła, Edyta
Silva, Cristiane Erdmann
Kopera, Edyta
Zdanowski, Konrad
Radecki, Jerzy
Radecka, Hanna
author_facet Mikuła, Edyta
Silva, Cristiane Erdmann
Kopera, Edyta
Zdanowski, Konrad
Radecki, Jerzy
Radecka, Hanna
author_sort Mikuła, Edyta
collection PubMed
description BACKGROUND: In this work, we report an electrochemical biosensor for the detection of anti-hemagglutinin antibodies against the swine virus H1N1 present in mice sera immunized with mixture of His(6)-H1 HA in monomeric and oligomeric form. The oriented immobilization of the recombinant His-tagged hemagglutinin (His(6)-H1 HA) consists of: (i) formation of a mixed layer of 4-mercaptobutanol (MBT) and the thiol derivative of dipyrromethene (DPM); (ii) complexation of Cu (II) by DPM; (iii) immobilization of His(6)-H1 HA via coordination bonds between Cu (II) sites from DPM–Cu (II) complex and imidazole nitrogen atoms of a histidine tag; (iv) filling free spaces with bovine serum albumin. The interactions between recombinant His(6)- H1 HA covalently attached to the electrode surface and the anti-hemagglutinin H1 antibodies present in mice sera were explored with Osteryoung square-wave voltammetry. RESULTS: This analytical device was able to detect the antibodies present in vaccinated mice sera diluted from 1 × 10(9) to 1 × 10(8) fold. CONCLUSIONS: The unprecedented sensitivity of described biosensor is much better than widely use ELISA test and other analytical methods for determination of antibodies against the influenza A viruses. It has been proved that redox active DPM-Cu (II) monolayer is a universal platform suitable for stable and oriented immobilization of any His-tagged sensing elements. Thus, this universal layer could be a base of numerous analytical devices suitable for detection of antibodies against different viruses.
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spelling pubmed-62189742018-11-08 Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice Mikuła, Edyta Silva, Cristiane Erdmann Kopera, Edyta Zdanowski, Konrad Radecki, Jerzy Radecka, Hanna BMC Vet Res Research Article BACKGROUND: In this work, we report an electrochemical biosensor for the detection of anti-hemagglutinin antibodies against the swine virus H1N1 present in mice sera immunized with mixture of His(6)-H1 HA in monomeric and oligomeric form. The oriented immobilization of the recombinant His-tagged hemagglutinin (His(6)-H1 HA) consists of: (i) formation of a mixed layer of 4-mercaptobutanol (MBT) and the thiol derivative of dipyrromethene (DPM); (ii) complexation of Cu (II) by DPM; (iii) immobilization of His(6)-H1 HA via coordination bonds between Cu (II) sites from DPM–Cu (II) complex and imidazole nitrogen atoms of a histidine tag; (iv) filling free spaces with bovine serum albumin. The interactions between recombinant His(6)- H1 HA covalently attached to the electrode surface and the anti-hemagglutinin H1 antibodies present in mice sera were explored with Osteryoung square-wave voltammetry. RESULTS: This analytical device was able to detect the antibodies present in vaccinated mice sera diluted from 1 × 10(9) to 1 × 10(8) fold. CONCLUSIONS: The unprecedented sensitivity of described biosensor is much better than widely use ELISA test and other analytical methods for determination of antibodies against the influenza A viruses. It has been proved that redox active DPM-Cu (II) monolayer is a universal platform suitable for stable and oriented immobilization of any His-tagged sensing elements. Thus, this universal layer could be a base of numerous analytical devices suitable for detection of antibodies against different viruses. BioMed Central 2018-11-06 /pmc/articles/PMC6218974/ /pubmed/30400888 http://dx.doi.org/10.1186/s12917-018-1668-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Mikuła, Edyta
Silva, Cristiane Erdmann
Kopera, Edyta
Zdanowski, Konrad
Radecki, Jerzy
Radecka, Hanna
Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice
title Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice
title_full Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice
title_fullStr Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice
title_full_unstemmed Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice
title_short Highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus H1N1 in sera of vaccinated mice
title_sort highly sensitive electrochemical biosensor based on redox - active monolayer for detection of anti-hemagglutinin antibodies against swine-origin influenza virus h1n1 in sera of vaccinated mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218974/
https://www.ncbi.nlm.nih.gov/pubmed/30400888
http://dx.doi.org/10.1186/s12917-018-1668-9
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