Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1

BACKGROUND: Pleckstrin homology-like domain family A member 1 (PHLDA1) is a tumor suppressor gene in gastric cancer, but its role regulated by circular RNAs (circRNAs) is not known. CircRNAs are important regulators in cancer growth and progression, however, the molecular roles of circRNAs in gastri...

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Detalles Bibliográficos
Autores principales: Wang, Liang, Shen, Jingyan, Jiang, Yushan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219040/
https://www.ncbi.nlm.nih.gov/pubmed/30410722
http://dx.doi.org/10.1186/s13578-018-0252-0
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author Wang, Liang
Shen, Jingyan
Jiang, Yushan
author_facet Wang, Liang
Shen, Jingyan
Jiang, Yushan
author_sort Wang, Liang
collection PubMed
description BACKGROUND: Pleckstrin homology-like domain family A member 1 (PHLDA1) is a tumor suppressor gene in gastric cancer, but its role regulated by circular RNAs (circRNAs) is not known. CircRNAs are important regulators in cancer growth and progression, however, the molecular roles of circRNAs in gastric cancer are rarely known. The study was aimed to investigate the role of circRNAs in regulating PHLDA1 expression in gastric cancer. RESULTS: The circRNA expression profile in the gastric cancer tissues by circRNA microarray showed that hsa_circ_0027599 (circ_0027599) was significantly down-regulated in gastric cancer patients and cells when comparing with the controls. Circ_0027599 overexpression suppressed gastric cancer cell proliferation and metastasis. By using bioinformatics tools and luciferase reporter assays, circ_0027599 was verified as a sponge of miR-101-3p.1 (miR-101) and suppressed cancer cell survival and metastasis. It was also verified that PHLDA1 was regulated by circ_0027599 in gastric cancer cells. CONCLUSIONS: The study uncovered that PHLDA1 was regulated by circ_0027599/miR-101, which suppressed gastric cancer survival and metastasis in gastric cancer.
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spelling pubmed-62190402018-11-08 Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1 Wang, Liang Shen, Jingyan Jiang, Yushan Cell Biosci Research BACKGROUND: Pleckstrin homology-like domain family A member 1 (PHLDA1) is a tumor suppressor gene in gastric cancer, but its role regulated by circular RNAs (circRNAs) is not known. CircRNAs are important regulators in cancer growth and progression, however, the molecular roles of circRNAs in gastric cancer are rarely known. The study was aimed to investigate the role of circRNAs in regulating PHLDA1 expression in gastric cancer. RESULTS: The circRNA expression profile in the gastric cancer tissues by circRNA microarray showed that hsa_circ_0027599 (circ_0027599) was significantly down-regulated in gastric cancer patients and cells when comparing with the controls. Circ_0027599 overexpression suppressed gastric cancer cell proliferation and metastasis. By using bioinformatics tools and luciferase reporter assays, circ_0027599 was verified as a sponge of miR-101-3p.1 (miR-101) and suppressed cancer cell survival and metastasis. It was also verified that PHLDA1 was regulated by circ_0027599 in gastric cancer cells. CONCLUSIONS: The study uncovered that PHLDA1 was regulated by circ_0027599/miR-101, which suppressed gastric cancer survival and metastasis in gastric cancer. BioMed Central 2018-11-06 /pmc/articles/PMC6219040/ /pubmed/30410722 http://dx.doi.org/10.1186/s13578-018-0252-0 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wang, Liang
Shen, Jingyan
Jiang, Yushan
Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1
title Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1
title_full Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1
title_fullStr Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1
title_full_unstemmed Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1
title_short Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1
title_sort circ_0027599/phdla1 suppresses gastric cancer progression by sponging mir-101-3p.1
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219040/
https://www.ncbi.nlm.nih.gov/pubmed/30410722
http://dx.doi.org/10.1186/s13578-018-0252-0
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AT jiangyushan circ0027599phdla1suppressesgastriccancerprogressionbyspongingmir1013p1

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