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Region-specific microRNA signatures in the human epididymis

The epithelium of the human epididymis maintains an appropriate luminal environment for sperm maturation that is essential for male fertility. Regional expression of small noncoding RNAs such as microRNAs contributes to segment-specific gene expression and differentiated functions. MicroRNA profiles...

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Autores principales: Browne, James A, Leir, Shih-Hsing, Eggener, Scott E, Harris, Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219309/
https://www.ncbi.nlm.nih.gov/pubmed/30058558
http://dx.doi.org/10.4103/aja.aja_40_18
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author Browne, James A
Leir, Shih-Hsing
Eggener, Scott E
Harris, Ann
author_facet Browne, James A
Leir, Shih-Hsing
Eggener, Scott E
Harris, Ann
author_sort Browne, James A
collection PubMed
description The epithelium of the human epididymis maintains an appropriate luminal environment for sperm maturation that is essential for male fertility. Regional expression of small noncoding RNAs such as microRNAs contributes to segment-specific gene expression and differentiated functions. MicroRNA profiles were reported in human epididymal tissues but not specifically in the epithelial cells derived from those regions. Here, we reveal miRNA signatures of primary cultures of caput, corpus, and cauda epididymis epithelial cells and of the tissues from which they were derived. We identify 324 epithelial cell-derived microRNAs and 259 tissue-derived microRNAs in the epididymis, some of which displayed regionalized expression patterns in cells and/or tissues. Caput cell-enriched miRNAs included miR-573 and miR-155. Cauda cell-enriched miRNAs included miR-1204 and miR-770. Next, we determined the gene ontology pathways associated with in silico predicted target genes of the differentially expressed miRNAs. The effect of androgen receptor stimulation on miRNA expression was also investigated. These data show novel epithelial cell-derived miRNAs that may regulate the expression of important gene networks that are responsible for the regionalized gene expression and function of the epididymis.
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spelling pubmed-62193092018-11-30 Region-specific microRNA signatures in the human epididymis Browne, James A Leir, Shih-Hsing Eggener, Scott E Harris, Ann Asian J Androl Original Article The epithelium of the human epididymis maintains an appropriate luminal environment for sperm maturation that is essential for male fertility. Regional expression of small noncoding RNAs such as microRNAs contributes to segment-specific gene expression and differentiated functions. MicroRNA profiles were reported in human epididymal tissues but not specifically in the epithelial cells derived from those regions. Here, we reveal miRNA signatures of primary cultures of caput, corpus, and cauda epididymis epithelial cells and of the tissues from which they were derived. We identify 324 epithelial cell-derived microRNAs and 259 tissue-derived microRNAs in the epididymis, some of which displayed regionalized expression patterns in cells and/or tissues. Caput cell-enriched miRNAs included miR-573 and miR-155. Cauda cell-enriched miRNAs included miR-1204 and miR-770. Next, we determined the gene ontology pathways associated with in silico predicted target genes of the differentially expressed miRNAs. The effect of androgen receptor stimulation on miRNA expression was also investigated. These data show novel epithelial cell-derived miRNAs that may regulate the expression of important gene networks that are responsible for the regionalized gene expression and function of the epididymis. Medknow Publications & Media Pvt Ltd 2018 2018-07-24 /pmc/articles/PMC6219309/ /pubmed/30058558 http://dx.doi.org/10.4103/aja.aja_40_18 Text en Copyright: © The Author(s)(2018) http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Browne, James A
Leir, Shih-Hsing
Eggener, Scott E
Harris, Ann
Region-specific microRNA signatures in the human epididymis
title Region-specific microRNA signatures in the human epididymis
title_full Region-specific microRNA signatures in the human epididymis
title_fullStr Region-specific microRNA signatures in the human epididymis
title_full_unstemmed Region-specific microRNA signatures in the human epididymis
title_short Region-specific microRNA signatures in the human epididymis
title_sort region-specific microrna signatures in the human epididymis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219309/
https://www.ncbi.nlm.nih.gov/pubmed/30058558
http://dx.doi.org/10.4103/aja.aja_40_18
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