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microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis

Genotoxic stress such as irradiation causes a temporary halt in tissue regeneration. The ability to regain regeneration depends on the type of cells that survived the assault. Previous studies showed that this propensity is usually held by the tissue-specific stem cells. However, stem cells cannot m...

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Detalles Bibliográficos
Autores principales: Volin, Marina, Zohar-Fux, Maayan, Gonen, Oren, Porat-Kuperstein, Lilach, Toledano, Hila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219711/
https://www.ncbi.nlm.nih.gov/pubmed/30093492
http://dx.doi.org/10.1083/jcb.201711098
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author Volin, Marina
Zohar-Fux, Maayan
Gonen, Oren
Porat-Kuperstein, Lilach
Toledano, Hila
author_facet Volin, Marina
Zohar-Fux, Maayan
Gonen, Oren
Porat-Kuperstein, Lilach
Toledano, Hila
author_sort Volin, Marina
collection PubMed
description Genotoxic stress such as irradiation causes a temporary halt in tissue regeneration. The ability to regain regeneration depends on the type of cells that survived the assault. Previous studies showed that this propensity is usually held by the tissue-specific stem cells. However, stem cells cannot maintain their unique properties without the support of their surrounding niche cells. In this study, we show that exposure of Drosophila melanogaster to extremely high levels of irradiation temporarily arrests spermatogenesis and kills half of the stem cells. In marked contrast, the hub cells that constitute a major component of the niche remain completely intact. We further show that this atypical resistance to cell death relies on the expression of certain antiapoptotic microRNAs (miRNAs) that are selectively expressed in the hub and keep the cells inert to apoptotic stress signals. We propose that at the tissue level, protection of a specific group of niche cells from apoptosis underlies ongoing stem cell turnover and tissue regeneration.
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spelling pubmed-62197112019-05-05 microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis Volin, Marina Zohar-Fux, Maayan Gonen, Oren Porat-Kuperstein, Lilach Toledano, Hila J Cell Biol Research Articles Genotoxic stress such as irradiation causes a temporary halt in tissue regeneration. The ability to regain regeneration depends on the type of cells that survived the assault. Previous studies showed that this propensity is usually held by the tissue-specific stem cells. However, stem cells cannot maintain their unique properties without the support of their surrounding niche cells. In this study, we show that exposure of Drosophila melanogaster to extremely high levels of irradiation temporarily arrests spermatogenesis and kills half of the stem cells. In marked contrast, the hub cells that constitute a major component of the niche remain completely intact. We further show that this atypical resistance to cell death relies on the expression of certain antiapoptotic microRNAs (miRNAs) that are selectively expressed in the hub and keep the cells inert to apoptotic stress signals. We propose that at the tissue level, protection of a specific group of niche cells from apoptosis underlies ongoing stem cell turnover and tissue regeneration. Rockefeller University Press 2018-11-05 /pmc/articles/PMC6219711/ /pubmed/30093492 http://dx.doi.org/10.1083/jcb.201711098 Text en © 2018 Volin et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Volin, Marina
Zohar-Fux, Maayan
Gonen, Oren
Porat-Kuperstein, Lilach
Toledano, Hila
microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis
title microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis
title_full microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis
title_fullStr microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis
title_full_unstemmed microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis
title_short microRNAs selectively protect hub cells of the germline stem cell niche from apoptosis
title_sort micrornas selectively protect hub cells of the germline stem cell niche from apoptosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219711/
https://www.ncbi.nlm.nih.gov/pubmed/30093492
http://dx.doi.org/10.1083/jcb.201711098
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