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Multiple roles of lymphatic vessels in peripheral lymph node development
The mammalian lymphatic system consists of strategically located lymph nodes (LNs) embedded into a lymphatic vascular network. Mechanisms underlying development of this highly organized system are not fully understood. Using high-resolution imaging, we show that lymphoid tissue inducer (LTi) cells i...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219737/ https://www.ncbi.nlm.nih.gov/pubmed/30355615 http://dx.doi.org/10.1084/jem.20180217 |
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author | Bovay, Esther Sabine, Amélie Prat-Luri, Borja Kim, Sudong Son, Kyungmin Willrodt, Ann-Helen Olsson, Cecilia Halin, Cornelia Kiefer, Friedemann Betsholtz, Christer Jeon, Noo Li Luther, Sanjiv A. Petrova, Tatiana V. |
author_facet | Bovay, Esther Sabine, Amélie Prat-Luri, Borja Kim, Sudong Son, Kyungmin Willrodt, Ann-Helen Olsson, Cecilia Halin, Cornelia Kiefer, Friedemann Betsholtz, Christer Jeon, Noo Li Luther, Sanjiv A. Petrova, Tatiana V. |
author_sort | Bovay, Esther |
collection | PubMed |
description | The mammalian lymphatic system consists of strategically located lymph nodes (LNs) embedded into a lymphatic vascular network. Mechanisms underlying development of this highly organized system are not fully understood. Using high-resolution imaging, we show that lymphoid tissue inducer (LTi) cells initially transmigrate from veins at LN development sites using gaps in venous mural coverage. This process is independent of lymphatic vasculature, but lymphatic vessels are indispensable for the transport of LTi cells that egress from blood capillaries elsewhere and serve as an essential LN expansion reservoir. At later stages, lymphatic collecting vessels ensure efficient LTi cell transport and formation of the LN capsule and subcapsular sinus. Perinodal lymphatics also promote local interstitial flow, which cooperates with lymphotoxin-β signaling to amplify stromal CXCL13 production and thereby promote LTi cell retention. Our data unify previous models of LN development by showing that lymphatics intervene at multiple points to assist LN expansion and identify a new role for mechanical forces in LN development. |
format | Online Article Text |
id | pubmed-6219737 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-62197372019-05-05 Multiple roles of lymphatic vessels in peripheral lymph node development Bovay, Esther Sabine, Amélie Prat-Luri, Borja Kim, Sudong Son, Kyungmin Willrodt, Ann-Helen Olsson, Cecilia Halin, Cornelia Kiefer, Friedemann Betsholtz, Christer Jeon, Noo Li Luther, Sanjiv A. Petrova, Tatiana V. J Exp Med Research Articles The mammalian lymphatic system consists of strategically located lymph nodes (LNs) embedded into a lymphatic vascular network. Mechanisms underlying development of this highly organized system are not fully understood. Using high-resolution imaging, we show that lymphoid tissue inducer (LTi) cells initially transmigrate from veins at LN development sites using gaps in venous mural coverage. This process is independent of lymphatic vasculature, but lymphatic vessels are indispensable for the transport of LTi cells that egress from blood capillaries elsewhere and serve as an essential LN expansion reservoir. At later stages, lymphatic collecting vessels ensure efficient LTi cell transport and formation of the LN capsule and subcapsular sinus. Perinodal lymphatics also promote local interstitial flow, which cooperates with lymphotoxin-β signaling to amplify stromal CXCL13 production and thereby promote LTi cell retention. Our data unify previous models of LN development by showing that lymphatics intervene at multiple points to assist LN expansion and identify a new role for mechanical forces in LN development. Rockefeller University Press 2018-11-05 /pmc/articles/PMC6219737/ /pubmed/30355615 http://dx.doi.org/10.1084/jem.20180217 Text en © 2018 Bovay et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Bovay, Esther Sabine, Amélie Prat-Luri, Borja Kim, Sudong Son, Kyungmin Willrodt, Ann-Helen Olsson, Cecilia Halin, Cornelia Kiefer, Friedemann Betsholtz, Christer Jeon, Noo Li Luther, Sanjiv A. Petrova, Tatiana V. Multiple roles of lymphatic vessels in peripheral lymph node development |
title | Multiple roles of lymphatic vessels in peripheral lymph node development |
title_full | Multiple roles of lymphatic vessels in peripheral lymph node development |
title_fullStr | Multiple roles of lymphatic vessels in peripheral lymph node development |
title_full_unstemmed | Multiple roles of lymphatic vessels in peripheral lymph node development |
title_short | Multiple roles of lymphatic vessels in peripheral lymph node development |
title_sort | multiple roles of lymphatic vessels in peripheral lymph node development |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219737/ https://www.ncbi.nlm.nih.gov/pubmed/30355615 http://dx.doi.org/10.1084/jem.20180217 |
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