Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats

The aim of this study, was to determine the effect of sulfasalazine for different periods of time reduces disseminated intravascular coagulation, inflammation and organ damages by inhibiting the nuclear factor kappa beta pathway. The study was performed with 30 Wistar albino rats and the groups were...

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Autores principales: Dik, Burak, Sonmez, Gonca, Faki, Hatice Eser, Bahcivan, Emre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219878/
https://www.ncbi.nlm.nih.gov/pubmed/29731490
http://dx.doi.org/10.1538/expanim.18-0029
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author Dik, Burak
Sonmez, Gonca
Faki, Hatice Eser
Bahcivan, Emre
author_facet Dik, Burak
Sonmez, Gonca
Faki, Hatice Eser
Bahcivan, Emre
author_sort Dik, Burak
collection PubMed
description The aim of this study, was to determine the effect of sulfasalazine for different periods of time reduces disseminated intravascular coagulation, inflammation and organ damages by inhibiting the nuclear factor kappa beta pathway. The study was performed with 30 Wistar albino rats and the groups were established as Control group, LPS group; endotoxemia was induced with LPS, SL5 group: sulfasalazine (300 mg/kg, single dose daily) was administered for 5 days before the LPS-induced endotoxemia, and LS group: sulfasalazine (300 mg/kg, single dose) was administered similtenously with LPS. Hemogram, biochemical, cytokine (IL-1β, IL-6, IL-10, TNF-α) and acute phase proteins (HPT, SAA, PGE2) analyzes and oxidative status values were measured from blood samples at 3 and 6 h after the last applications in the all groups. The rats were euthanized at 6 h and mRNA levels of BCL2 and BAX genes were examined from liver and brain tissues. Sulfasalazine reduced the increased IL-1β, IL-6, TNF-α and PGE(2) levels and significantly increased anti-inflammatory cytokine IL-10 levels. In addition, decreasing of ATIII level was prevented in the SL5 group, and decreasing of fibrinogen levels were prevented in the LS and SL5 groups within first 3 h. In LPS group, leukocyte and thrombocyte levels were decreased, however sulfasalazine application inhibited decreases of leukocyte levels in LS and SL5 groups. In addition, sulfasalazine inhibited the decrease of total antioxidant capacity and unchanged apoptosis in brain and liver. In conclusion, the use of sulfasalazine in different durations reduce the excessive inflammation of endotoxemia cases.
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spelling pubmed-62198782018-11-09 Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats Dik, Burak Sonmez, Gonca Faki, Hatice Eser Bahcivan, Emre Exp Anim Original The aim of this study, was to determine the effect of sulfasalazine for different periods of time reduces disseminated intravascular coagulation, inflammation and organ damages by inhibiting the nuclear factor kappa beta pathway. The study was performed with 30 Wistar albino rats and the groups were established as Control group, LPS group; endotoxemia was induced with LPS, SL5 group: sulfasalazine (300 mg/kg, single dose daily) was administered for 5 days before the LPS-induced endotoxemia, and LS group: sulfasalazine (300 mg/kg, single dose) was administered similtenously with LPS. Hemogram, biochemical, cytokine (IL-1β, IL-6, IL-10, TNF-α) and acute phase proteins (HPT, SAA, PGE2) analyzes and oxidative status values were measured from blood samples at 3 and 6 h after the last applications in the all groups. The rats were euthanized at 6 h and mRNA levels of BCL2 and BAX genes were examined from liver and brain tissues. Sulfasalazine reduced the increased IL-1β, IL-6, TNF-α and PGE(2) levels and significantly increased anti-inflammatory cytokine IL-10 levels. In addition, decreasing of ATIII level was prevented in the SL5 group, and decreasing of fibrinogen levels were prevented in the LS and SL5 groups within first 3 h. In LPS group, leukocyte and thrombocyte levels were decreased, however sulfasalazine application inhibited decreases of leukocyte levels in LS and SL5 groups. In addition, sulfasalazine inhibited the decrease of total antioxidant capacity and unchanged apoptosis in brain and liver. In conclusion, the use of sulfasalazine in different durations reduce the excessive inflammation of endotoxemia cases. Japanese Association for Laboratory Animal Science 2018-05-02 2018 /pmc/articles/PMC6219878/ /pubmed/29731490 http://dx.doi.org/10.1538/expanim.18-0029 Text en ©2018 Japanese Association for Laboratory Animal Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original
Dik, Burak
Sonmez, Gonca
Faki, Hatice Eser
Bahcivan, Emre
Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats
title Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats
title_full Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats
title_fullStr Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats
title_full_unstemmed Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats
title_short Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats
title_sort sulfasalazine treatment can cause a positive effect on lps-induced endotoxic rats
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219878/
https://www.ncbi.nlm.nih.gov/pubmed/29731490
http://dx.doi.org/10.1538/expanim.18-0029
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