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The Lipid Mediator Resolvin D1 Reduces the Skin Inflammation and Oxidative Stress Induced by UV Irradiation in Hairless Mice
UV irradiation-induced oxidative stress and inflammation contribute to the development of skin diseases. Therefore, targeting oxidative stress and inflammation might contribute to reduce skin diseases. Resolvin D1 (RvD1) is a bioactive metabolite generated during inflammation to actively orchestrate...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6220064/ https://www.ncbi.nlm.nih.gov/pubmed/30429790 http://dx.doi.org/10.3389/fphar.2018.01242 |
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author | Saito, Priscila Melo, Cristina P. B. Martinez, Renata M. Fattori, Victor Cezar, Talita L. C. Pinto, Ingrid C. Bussmann, Allan J. C. Vignoli, Josiane A. Georgetti, Sandra R. Baracat, Marcela M. Verri, Waldiceu A. Casagrande, Rubia |
author_facet | Saito, Priscila Melo, Cristina P. B. Martinez, Renata M. Fattori, Victor Cezar, Talita L. C. Pinto, Ingrid C. Bussmann, Allan J. C. Vignoli, Josiane A. Georgetti, Sandra R. Baracat, Marcela M. Verri, Waldiceu A. Casagrande, Rubia |
author_sort | Saito, Priscila |
collection | PubMed |
description | UV irradiation-induced oxidative stress and inflammation contribute to the development of skin diseases. Therefore, targeting oxidative stress and inflammation might contribute to reduce skin diseases. Resolvin D1 (RvD1) is a bioactive metabolite generated during inflammation to actively orchestrate the resolution of inflammation. However, the therapeutic potential of RvD1 in UVB skin inflammation remains undetermined, which was, therefore, the aim of the present study. The intraperitoneal treatment with RvD1 (3-100 ng/mouse) reduced UVB irradiation-induced skin edema, myeloperoxidase activity, matrix metalloproteinase 9 activity, and reduced glutathione depletion with consistent effects observed with the dose of 30 ng/mouse, which was selected to the following experiments. RvD1 inhibited UVB reduction of catalase activity, and hydroperoxide formation, superoxide anion production, and gp91phox mRNA expression. RvD1 also increased the Nrf2 and its downstream targets NQO1 and HO-1 mRNA expression. Regarding cytokines, RvD1 inhibited UVB-induced production of IL-1β, IL-6, IL-33, TNF-α, TGF-β, and IL-10. These immuno-biochemical alterations by RvD1 treatment had as consequence the reduction of UVB-induced epidermal thickness, sunburn and mast cell counts, and collagen degradation. Therefore, RvD1 inhibited UVB-induced skin oxidative stress and inflammation, rendering this resolving lipid mediator as a promising therapeutic agent. |
format | Online Article Text |
id | pubmed-6220064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-62200642018-11-14 The Lipid Mediator Resolvin D1 Reduces the Skin Inflammation and Oxidative Stress Induced by UV Irradiation in Hairless Mice Saito, Priscila Melo, Cristina P. B. Martinez, Renata M. Fattori, Victor Cezar, Talita L. C. Pinto, Ingrid C. Bussmann, Allan J. C. Vignoli, Josiane A. Georgetti, Sandra R. Baracat, Marcela M. Verri, Waldiceu A. Casagrande, Rubia Front Pharmacol Pharmacology UV irradiation-induced oxidative stress and inflammation contribute to the development of skin diseases. Therefore, targeting oxidative stress and inflammation might contribute to reduce skin diseases. Resolvin D1 (RvD1) is a bioactive metabolite generated during inflammation to actively orchestrate the resolution of inflammation. However, the therapeutic potential of RvD1 in UVB skin inflammation remains undetermined, which was, therefore, the aim of the present study. The intraperitoneal treatment with RvD1 (3-100 ng/mouse) reduced UVB irradiation-induced skin edema, myeloperoxidase activity, matrix metalloproteinase 9 activity, and reduced glutathione depletion with consistent effects observed with the dose of 30 ng/mouse, which was selected to the following experiments. RvD1 inhibited UVB reduction of catalase activity, and hydroperoxide formation, superoxide anion production, and gp91phox mRNA expression. RvD1 also increased the Nrf2 and its downstream targets NQO1 and HO-1 mRNA expression. Regarding cytokines, RvD1 inhibited UVB-induced production of IL-1β, IL-6, IL-33, TNF-α, TGF-β, and IL-10. These immuno-biochemical alterations by RvD1 treatment had as consequence the reduction of UVB-induced epidermal thickness, sunburn and mast cell counts, and collagen degradation. Therefore, RvD1 inhibited UVB-induced skin oxidative stress and inflammation, rendering this resolving lipid mediator as a promising therapeutic agent. Frontiers Media S.A. 2018-10-31 /pmc/articles/PMC6220064/ /pubmed/30429790 http://dx.doi.org/10.3389/fphar.2018.01242 Text en Copyright © 2018 Saito, Melo, Martinez, Fattori, Cezar, Pinto, Bussmann, Vignoli, Georgetti, Baracat, Verri and Casagrande. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Saito, Priscila Melo, Cristina P. B. Martinez, Renata M. Fattori, Victor Cezar, Talita L. C. Pinto, Ingrid C. Bussmann, Allan J. C. Vignoli, Josiane A. Georgetti, Sandra R. Baracat, Marcela M. Verri, Waldiceu A. Casagrande, Rubia The Lipid Mediator Resolvin D1 Reduces the Skin Inflammation and Oxidative Stress Induced by UV Irradiation in Hairless Mice |
title | The Lipid Mediator Resolvin D1 Reduces the Skin Inflammation and Oxidative Stress Induced by UV Irradiation in Hairless Mice |
title_full | The Lipid Mediator Resolvin D1 Reduces the Skin Inflammation and Oxidative Stress Induced by UV Irradiation in Hairless Mice |
title_fullStr | The Lipid Mediator Resolvin D1 Reduces the Skin Inflammation and Oxidative Stress Induced by UV Irradiation in Hairless Mice |
title_full_unstemmed | The Lipid Mediator Resolvin D1 Reduces the Skin Inflammation and Oxidative Stress Induced by UV Irradiation in Hairless Mice |
title_short | The Lipid Mediator Resolvin D1 Reduces the Skin Inflammation and Oxidative Stress Induced by UV Irradiation in Hairless Mice |
title_sort | lipid mediator resolvin d1 reduces the skin inflammation and oxidative stress induced by uv irradiation in hairless mice |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6220064/ https://www.ncbi.nlm.nih.gov/pubmed/30429790 http://dx.doi.org/10.3389/fphar.2018.01242 |
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