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Thermometry of photosensitive and optically induced electrokinetics chips

Optically induced electrokinetics (OEK)-based technologies, which integrate the high-resolution dynamic addressability of optical tweezers and the high-throughput capability of electrokinetic forces, have been widely used to manipulate, assemble, and separate biological and non-biological entities i...

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Autores principales: Wang, Feifei, Liu, Lianqing, Li, Gongxin, Li, Pan, Wen, Yangdong, Zhang, Guanglie, Wang, Yuechao, Lee, Gwo-Bin, Li, Wen Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6220187/
https://www.ncbi.nlm.nih.gov/pubmed/31057914
http://dx.doi.org/10.1038/s41378-018-0029-y
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author Wang, Feifei
Liu, Lianqing
Li, Gongxin
Li, Pan
Wen, Yangdong
Zhang, Guanglie
Wang, Yuechao
Lee, Gwo-Bin
Li, Wen Jung
author_facet Wang, Feifei
Liu, Lianqing
Li, Gongxin
Li, Pan
Wen, Yangdong
Zhang, Guanglie
Wang, Yuechao
Lee, Gwo-Bin
Li, Wen Jung
author_sort Wang, Feifei
collection PubMed
description Optically induced electrokinetics (OEK)-based technologies, which integrate the high-resolution dynamic addressability of optical tweezers and the high-throughput capability of electrokinetic forces, have been widely used to manipulate, assemble, and separate biological and non-biological entities in parallel on scales ranging from micrometers to nanometers. However, simultaneously introducing optical and electrical energy into an OEK chip may induce a problematic temperature increase, which poses the potential risk of exceeding physiological conditions and thus inducing variations in cell behavior or activity or even irreversible cell damage during bio-manipulation. Here, we systematically measure the temperature distribution and changes in an OEK chip arising from the projected images and applied alternating current (AC) voltage using an infrared camera. We have found that the average temperature of a projected area is influenced by the light color, total illumination area, ratio of lighted regions to the total controlled areas, and amplitude of the AC voltage. As an example, optically induced thermocapillary flow is triggered by the light image-induced temperature gradient on a photosensitive substrate to realize fluidic hydrogel patterning. Our studies show that the projected light pattern needs to be properly designed to satisfy specific application requirements, especially for applications related to cell manipulation and assembly.
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spelling pubmed-62201872019-05-03 Thermometry of photosensitive and optically induced electrokinetics chips Wang, Feifei Liu, Lianqing Li, Gongxin Li, Pan Wen, Yangdong Zhang, Guanglie Wang, Yuechao Lee, Gwo-Bin Li, Wen Jung Microsyst Nanoeng Article Optically induced electrokinetics (OEK)-based technologies, which integrate the high-resolution dynamic addressability of optical tweezers and the high-throughput capability of electrokinetic forces, have been widely used to manipulate, assemble, and separate biological and non-biological entities in parallel on scales ranging from micrometers to nanometers. However, simultaneously introducing optical and electrical energy into an OEK chip may induce a problematic temperature increase, which poses the potential risk of exceeding physiological conditions and thus inducing variations in cell behavior or activity or even irreversible cell damage during bio-manipulation. Here, we systematically measure the temperature distribution and changes in an OEK chip arising from the projected images and applied alternating current (AC) voltage using an infrared camera. We have found that the average temperature of a projected area is influenced by the light color, total illumination area, ratio of lighted regions to the total controlled areas, and amplitude of the AC voltage. As an example, optically induced thermocapillary flow is triggered by the light image-induced temperature gradient on a photosensitive substrate to realize fluidic hydrogel patterning. Our studies show that the projected light pattern needs to be properly designed to satisfy specific application requirements, especially for applications related to cell manipulation and assembly. Nature Publishing Group UK 2018-08-27 /pmc/articles/PMC6220187/ /pubmed/31057914 http://dx.doi.org/10.1038/s41378-018-0029-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Feifei
Liu, Lianqing
Li, Gongxin
Li, Pan
Wen, Yangdong
Zhang, Guanglie
Wang, Yuechao
Lee, Gwo-Bin
Li, Wen Jung
Thermometry of photosensitive and optically induced electrokinetics chips
title Thermometry of photosensitive and optically induced electrokinetics chips
title_full Thermometry of photosensitive and optically induced electrokinetics chips
title_fullStr Thermometry of photosensitive and optically induced electrokinetics chips
title_full_unstemmed Thermometry of photosensitive and optically induced electrokinetics chips
title_short Thermometry of photosensitive and optically induced electrokinetics chips
title_sort thermometry of photosensitive and optically induced electrokinetics chips
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6220187/
https://www.ncbi.nlm.nih.gov/pubmed/31057914
http://dx.doi.org/10.1038/s41378-018-0029-y
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