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Effects of CRISPR/Cas9 dosage on TICAM1 and RBL gene mutation rate, embryonic development, hatchability and fry survival in channel catfish

The current study was conducted to assess the effects of microinjection of different dosages of guide RNA (gRNA)/Cas9 protein on the mutation rate, embryo survival, embryonic development, hatchability and early fry survival in channel catfish, Ictalurus punctatus. Guide RNAs targeting two of the cha...

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Detalles Bibliográficos
Autores principales:	Elaswad, Ahmed, Khalil, Karim, Ye, Zhi, Liu, Zhanjiang, Liu, Shikai, Peatman, Eric, Odin, Ramjie, Vo, Khoi, Drescher, David, Gosh, Kamal, Qin, Guyu, Bugg, William, Backenstose, Nathan, Dunham, Rex
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6220201/ https://www.ncbi.nlm.nih.gov/pubmed/30405210 http://dx.doi.org/10.1038/s41598-018-34738-4

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