Genetic, Reproductive and Hematological Toxicity Induced in Mice Exposed to Leachates from Petrol, Diesel and Kerosene Dispensing Sites

BACKGROUND. With a population of over 165,000,000, growing at an average rate of 2.7% per annum and an economic growth rate of about 5.7% in the past five years, the market for refined petroleum products in Nigeria is growing. As a result, the number of filling stations is increasing. OBJECTIVES. The present study evaluated the reproductive and genetic toxicity of simulated leachate of soil from petrol, diesel and kerosene dispensing sites in a filling station using the murine sperm abnormality test, sperm count and bone marrow micronucleus assay. METHODS. Simulated leachate of soil collected from petrol, diesel and kerosene dispensing sites in a filling station was intraperitoneally administered to mice at different concentrations. Bone marrow micronucleus assay was carried out after 5-days exposure, while sperm morphology assay was carried out 35 days from the first day of exposure. Alterations to hematological parameters were evaluated and physico-chemical analysis of the leachate samples was also carried out. RESULTS. The results showed a significant (p<0.05) concentration-dependent increase in abnormal sperm cells and decrease in mean sperm count in all the samples tested. Increased induction of micronucleated polychromatic erythrocytes was observed in the exposed mice. Hematological analysis showed a significant (p<0.05) increase in the values of white blood cell count (WBC), lymphocytes, neutrophils, monocytes, eosinophils and mean corpuscular volume (MCV), while a significant (p<0.05) reduction in basophils, hemoglobin, mean corpuscular hemoglobin (MCH), packed cell volume and mean corpuscular hemoglobin concentration (MCHC) values were observed. DISCUSSION. In the present study, simulated leachates from soil obtained from petrol, diesel and kerosene dispensing sites were shown to cause genomic disruptions in germ and somatic cells, and hematotoxicity in an animal model. These observed reproductive, genetic and hemato-toxicities are believed to be caused by the presence of lead, copper, mercury, polycyclic aromatic hydrocarbons, and benzene in the samples. CONCLUSIONS. This study showed the negative impact of petroleum products in the contamination of soil, with a capability of inducing genetic damage in somatic and germ cells of exposed plants and animals. ETHICS APPROVAL. The study was approved by the ethical committee of the Federal University of Technology, Akure, Ondo State, Nigeria.