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A Promising Copper(II) Complex as Antifungal and Antibiofilm Drug against Yeast Infection

The high mortality rate of candidemia and the limited option for the treatment of Candida spp. infection have been driving the search for new molecules with antifungal property. In this context, coordination complexes of metal ions and ligands appear to be important. Therefore, this study aimed to s...

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Autores principales: Gomes da Silva Dantas, Fabiana, Araújo de Almeida-Apolonio, Adriana, Pires de Araújo, Renata, Regiane Vizolli Favarin, Lis, Fukuda de Castilho, Pamella, de Oliveira Galvão, Fernanda, Inez Estivalet Svidzinski, Terezinha, Antônio Casagrande, Gleison, Mari Pires de Oliveira, Kelly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222317/
https://www.ncbi.nlm.nih.gov/pubmed/30049937
http://dx.doi.org/10.3390/molecules23081856
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author Gomes da Silva Dantas, Fabiana
Araújo de Almeida-Apolonio, Adriana
Pires de Araújo, Renata
Regiane Vizolli Favarin, Lis
Fukuda de Castilho, Pamella
de Oliveira Galvão, Fernanda
Inez Estivalet Svidzinski, Terezinha
Antônio Casagrande, Gleison
Mari Pires de Oliveira, Kelly
author_facet Gomes da Silva Dantas, Fabiana
Araújo de Almeida-Apolonio, Adriana
Pires de Araújo, Renata
Regiane Vizolli Favarin, Lis
Fukuda de Castilho, Pamella
de Oliveira Galvão, Fernanda
Inez Estivalet Svidzinski, Terezinha
Antônio Casagrande, Gleison
Mari Pires de Oliveira, Kelly
author_sort Gomes da Silva Dantas, Fabiana
collection PubMed
description The high mortality rate of candidemia and the limited option for the treatment of Candida spp. infection have been driving the search for new molecules with antifungal property. In this context, coordination complexes of metal ions and ligands appear to be important. Therefore, this study aimed to synthesize two new copper(II) complexes with 2-thiouracil and 6-methyl-2-thiouracil ligands and to evaluate their mutagenic potential and antifungal activity against Candida. The complexes were synthesized and characterized by infrared vibrational spectroscopy, CHN elemental analysis, UV-Vis experiments and ESI-HRMS spectrometry studies. The antifungal activity was evaluated by broth microdilution against 21 clinical isolates of Candida species. The mutagenic potential was evaluated by the Ames test. The complexes were Cu(Bipy)Cl(2)(thiouracil) (Complex 1) and Cu(Bipy)Cl(2)(6-methylthiouracil) (Complex 2). Complex 1 showed fungicidal and fungistatic activities against all isolates. Furthermore, the Minimum Inhibitory Concentration (MIC) from 31 to 125 µg/mL and inhibition percentage of 9.9% against the biofilms of C. krusei and C. glabrata were demonstrated. At the concentrations tested, complex 1 exhibited no mutagenic potential. Complex 2 and the free ligands exhibited no antifungal activity at the concentrations evaluated. Since complex 1 presented antifungal activity against all the tested isolates and no mutagenic potential, it could be proposed as a potential new drug for anti-Candida therapy.
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spelling pubmed-62223172018-11-13 A Promising Copper(II) Complex as Antifungal and Antibiofilm Drug against Yeast Infection Gomes da Silva Dantas, Fabiana Araújo de Almeida-Apolonio, Adriana Pires de Araújo, Renata Regiane Vizolli Favarin, Lis Fukuda de Castilho, Pamella de Oliveira Galvão, Fernanda Inez Estivalet Svidzinski, Terezinha Antônio Casagrande, Gleison Mari Pires de Oliveira, Kelly Molecules Article The high mortality rate of candidemia and the limited option for the treatment of Candida spp. infection have been driving the search for new molecules with antifungal property. In this context, coordination complexes of metal ions and ligands appear to be important. Therefore, this study aimed to synthesize two new copper(II) complexes with 2-thiouracil and 6-methyl-2-thiouracil ligands and to evaluate their mutagenic potential and antifungal activity against Candida. The complexes were synthesized and characterized by infrared vibrational spectroscopy, CHN elemental analysis, UV-Vis experiments and ESI-HRMS spectrometry studies. The antifungal activity was evaluated by broth microdilution against 21 clinical isolates of Candida species. The mutagenic potential was evaluated by the Ames test. The complexes were Cu(Bipy)Cl(2)(thiouracil) (Complex 1) and Cu(Bipy)Cl(2)(6-methylthiouracil) (Complex 2). Complex 1 showed fungicidal and fungistatic activities against all isolates. Furthermore, the Minimum Inhibitory Concentration (MIC) from 31 to 125 µg/mL and inhibition percentage of 9.9% against the biofilms of C. krusei and C. glabrata were demonstrated. At the concentrations tested, complex 1 exhibited no mutagenic potential. Complex 2 and the free ligands exhibited no antifungal activity at the concentrations evaluated. Since complex 1 presented antifungal activity against all the tested isolates and no mutagenic potential, it could be proposed as a potential new drug for anti-Candida therapy. MDPI 2018-07-26 /pmc/articles/PMC6222317/ /pubmed/30049937 http://dx.doi.org/10.3390/molecules23081856 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gomes da Silva Dantas, Fabiana
Araújo de Almeida-Apolonio, Adriana
Pires de Araújo, Renata
Regiane Vizolli Favarin, Lis
Fukuda de Castilho, Pamella
de Oliveira Galvão, Fernanda
Inez Estivalet Svidzinski, Terezinha
Antônio Casagrande, Gleison
Mari Pires de Oliveira, Kelly
A Promising Copper(II) Complex as Antifungal and Antibiofilm Drug against Yeast Infection
title A Promising Copper(II) Complex as Antifungal and Antibiofilm Drug against Yeast Infection
title_full A Promising Copper(II) Complex as Antifungal and Antibiofilm Drug against Yeast Infection
title_fullStr A Promising Copper(II) Complex as Antifungal and Antibiofilm Drug against Yeast Infection
title_full_unstemmed A Promising Copper(II) Complex as Antifungal and Antibiofilm Drug against Yeast Infection
title_short A Promising Copper(II) Complex as Antifungal and Antibiofilm Drug against Yeast Infection
title_sort promising copper(ii) complex as antifungal and antibiofilm drug against yeast infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222317/
https://www.ncbi.nlm.nih.gov/pubmed/30049937
http://dx.doi.org/10.3390/molecules23081856
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