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Phenolic Compounds and Bioactivity of Cytisus villosus Pourr.

The present study focuses on the chemical composition, antioxidant, antimicrobial, and antiproliferative activities of the ethyl acetate and aqueous extracts obtained from the aerial parts of Cytisus villosus Pourr. HPLC-DAD-ESI/MSn was used to identify the phenolic compounds, being (epi)gallocatech...

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Autores principales: Bouziane, Amel, Bakchiche, Boulanouar, Dias, Maria Inês, Barros, Lillian, Ferreira, Isabel C.F.R., AlSalamat, Husam A., Bardaweel, Sanaa K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222350/
https://www.ncbi.nlm.nih.gov/pubmed/30103409
http://dx.doi.org/10.3390/molecules23081994
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author Bouziane, Amel
Bakchiche, Boulanouar
Dias, Maria Inês
Barros, Lillian
Ferreira, Isabel C.F.R.
AlSalamat, Husam A.
Bardaweel, Sanaa K.
author_facet Bouziane, Amel
Bakchiche, Boulanouar
Dias, Maria Inês
Barros, Lillian
Ferreira, Isabel C.F.R.
AlSalamat, Husam A.
Bardaweel, Sanaa K.
author_sort Bouziane, Amel
collection PubMed
description The present study focuses on the chemical composition, antioxidant, antimicrobial, and antiproliferative activities of the ethyl acetate and aqueous extracts obtained from the aerial parts of Cytisus villosus Pourr. HPLC-DAD-ESI/MSn was used to identify the phenolic compounds, being (epi)gallocatechin dimer the major compound (111 ± 5 µg/g·dw) in the aqueous extract, while myricetin-O-rhamnoside (226 ± 9 µg/g·dw) was the main molecule in the ethyl acetate extract. Both extracts exhibited good scavenging activities against DPPH radical (EC(50) µg/mL of 59 ± 2 and 31 ± 2 for aqueous and ethyl acetate extracts, respectively). However, the ethyl acetate extract demonstrated more potent quenching activities than the aqueous extract. The antimicrobial activities were assessed on selected Gram-positive (Staphylococcus epidermidis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria, as well as on pathogenic fungus Candida glabrata. The extracts possessed selective and potent antimicrobial activities against the Gram-positive bacterium (IC(50) of 186 ± 9 μg/mL and 92 ± 3 μg/mL for aqueous and ethyl acetate extracts, respectively). Finally, C. villosus extracts were evaluated for their antiproliferative potential on three human cancer cell lines representing breast and colon cancers. Although both extracts demonstrated sufficient growth inhibition of the three different cell lines, the ethyl acetate extract exhibited higher activity (LD(50) values of 1.57 ± 0.06 mg/mL, 2.2 ± 0.1 mg/mL, and 3.2 ± 0.2 mg/mL for T47D, MCF-7, and HCT-116 cell lines). Both the extracts obtained from the aerial parts of C. villosus revealed very promising results and could be applied as functional agents in the food, pharmaceutical, and cosmeceutical industries.
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spelling pubmed-62223502018-11-13 Phenolic Compounds and Bioactivity of Cytisus villosus Pourr. Bouziane, Amel Bakchiche, Boulanouar Dias, Maria Inês Barros, Lillian Ferreira, Isabel C.F.R. AlSalamat, Husam A. Bardaweel, Sanaa K. Molecules Article The present study focuses on the chemical composition, antioxidant, antimicrobial, and antiproliferative activities of the ethyl acetate and aqueous extracts obtained from the aerial parts of Cytisus villosus Pourr. HPLC-DAD-ESI/MSn was used to identify the phenolic compounds, being (epi)gallocatechin dimer the major compound (111 ± 5 µg/g·dw) in the aqueous extract, while myricetin-O-rhamnoside (226 ± 9 µg/g·dw) was the main molecule in the ethyl acetate extract. Both extracts exhibited good scavenging activities against DPPH radical (EC(50) µg/mL of 59 ± 2 and 31 ± 2 for aqueous and ethyl acetate extracts, respectively). However, the ethyl acetate extract demonstrated more potent quenching activities than the aqueous extract. The antimicrobial activities were assessed on selected Gram-positive (Staphylococcus epidermidis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria, as well as on pathogenic fungus Candida glabrata. The extracts possessed selective and potent antimicrobial activities against the Gram-positive bacterium (IC(50) of 186 ± 9 μg/mL and 92 ± 3 μg/mL for aqueous and ethyl acetate extracts, respectively). Finally, C. villosus extracts were evaluated for their antiproliferative potential on three human cancer cell lines representing breast and colon cancers. Although both extracts demonstrated sufficient growth inhibition of the three different cell lines, the ethyl acetate extract exhibited higher activity (LD(50) values of 1.57 ± 0.06 mg/mL, 2.2 ± 0.1 mg/mL, and 3.2 ± 0.2 mg/mL for T47D, MCF-7, and HCT-116 cell lines). Both the extracts obtained from the aerial parts of C. villosus revealed very promising results and could be applied as functional agents in the food, pharmaceutical, and cosmeceutical industries. MDPI 2018-08-10 /pmc/articles/PMC6222350/ /pubmed/30103409 http://dx.doi.org/10.3390/molecules23081994 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bouziane, Amel
Bakchiche, Boulanouar
Dias, Maria Inês
Barros, Lillian
Ferreira, Isabel C.F.R.
AlSalamat, Husam A.
Bardaweel, Sanaa K.
Phenolic Compounds and Bioactivity of Cytisus villosus Pourr.
title Phenolic Compounds and Bioactivity of Cytisus villosus Pourr.
title_full Phenolic Compounds and Bioactivity of Cytisus villosus Pourr.
title_fullStr Phenolic Compounds and Bioactivity of Cytisus villosus Pourr.
title_full_unstemmed Phenolic Compounds and Bioactivity of Cytisus villosus Pourr.
title_short Phenolic Compounds and Bioactivity of Cytisus villosus Pourr.
title_sort phenolic compounds and bioactivity of cytisus villosus pourr.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222350/
https://www.ncbi.nlm.nih.gov/pubmed/30103409
http://dx.doi.org/10.3390/molecules23081994
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