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Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation
Isorhamnetin (ISOR), 3-O-methylquercetin, is a naturally occurring flavonoid in many plants. It is a metabolite derived from quercetin and is known to exert beneficial effects on the prevention of obesity. However, the molecular mechanism of action involved in ISOR-mediated mitochondrial biogenesis,...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222361/ https://www.ncbi.nlm.nih.gov/pubmed/30044453 http://dx.doi.org/10.3390/molecules23081853 |
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author | Lee, Mak-Soon Kim, Yangha |
author_facet | Lee, Mak-Soon Kim, Yangha |
author_sort | Lee, Mak-Soon |
collection | PubMed |
description | Isorhamnetin (ISOR), 3-O-methylquercetin, is a naturally occurring flavonoid in many plants. It is a metabolite derived from quercetin and is known to exert beneficial effects on the prevention of obesity. However, the molecular mechanism of action involved in ISOR-mediated mitochondrial biogenesis, and AMP-activated protein kinase (AMPK) activation in 3T3-L1 cells remains unclear. The aim of this study was to determine whether ISOR affected mitochondrial biogenesis and AMPK activation, during 3T3-L1 adipocyte differentiation. Intracellular lipid and triglyceride accumulation, and glycerol-3-phosphate dehydrogenase (GPDH) activity decreased in ISOR-treated cells. The mRNA levels of adipogenic genes, such as the proliferator-activated receptor-γ (PPAR-γ), and adipocyte protein 2 (aP2), were inhibited by ISOR. In contrast, mRNA levels of mitochondrial genes, such as peroxisome proliferator-activated reporter gamma coactivator-1α (PGC-1α), nuclear respiratory factor (NRF)-1, transcription factor A (Tfam), and carnitine palmitoyl transferase-1α (CPT-1α), were all stimulated by ISOR treatment. Mitochondria DNA (mtDNA) copy number and AMPK activity were also stimulated by ISOR. The results suggested that the mitochondrial biogenic effect of ISOR in adipocytes might have been associated with stimulation of mitochondrial gene expression, mtDNA replication, and AMPK activation. |
format | Online Article Text |
id | pubmed-6222361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62223612018-11-13 Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation Lee, Mak-Soon Kim, Yangha Molecules Article Isorhamnetin (ISOR), 3-O-methylquercetin, is a naturally occurring flavonoid in many plants. It is a metabolite derived from quercetin and is known to exert beneficial effects on the prevention of obesity. However, the molecular mechanism of action involved in ISOR-mediated mitochondrial biogenesis, and AMP-activated protein kinase (AMPK) activation in 3T3-L1 cells remains unclear. The aim of this study was to determine whether ISOR affected mitochondrial biogenesis and AMPK activation, during 3T3-L1 adipocyte differentiation. Intracellular lipid and triglyceride accumulation, and glycerol-3-phosphate dehydrogenase (GPDH) activity decreased in ISOR-treated cells. The mRNA levels of adipogenic genes, such as the proliferator-activated receptor-γ (PPAR-γ), and adipocyte protein 2 (aP2), were inhibited by ISOR. In contrast, mRNA levels of mitochondrial genes, such as peroxisome proliferator-activated reporter gamma coactivator-1α (PGC-1α), nuclear respiratory factor (NRF)-1, transcription factor A (Tfam), and carnitine palmitoyl transferase-1α (CPT-1α), were all stimulated by ISOR treatment. Mitochondria DNA (mtDNA) copy number and AMPK activity were also stimulated by ISOR. The results suggested that the mitochondrial biogenic effect of ISOR in adipocytes might have been associated with stimulation of mitochondrial gene expression, mtDNA replication, and AMPK activation. MDPI 2018-07-25 /pmc/articles/PMC6222361/ /pubmed/30044453 http://dx.doi.org/10.3390/molecules23081853 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Mak-Soon Kim, Yangha Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation |
title | Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation |
title_full | Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation |
title_fullStr | Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation |
title_full_unstemmed | Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation |
title_short | Effects of Isorhamnetin on Adipocyte Mitochondrial Biogenesis and AMPK Activation |
title_sort | effects of isorhamnetin on adipocyte mitochondrial biogenesis and ampk activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222361/ https://www.ncbi.nlm.nih.gov/pubmed/30044453 http://dx.doi.org/10.3390/molecules23081853 |
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