A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP

Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M′) as activators. The calculator-related...

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Detalles Bibliográficos
Autores principales: Zhang, Jingjing, Zhang, Shizhi, Niu, Chaoqun, Liu, Chen, Du, Jie, Chen, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222419/
https://www.ncbi.nlm.nih.gov/pubmed/30274237
http://dx.doi.org/10.3390/molecules23102494
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author Zhang, Jingjing
Zhang, Shizhi
Niu, Chaoqun
Liu, Chen
Du, Jie
Chen, Yong
author_facet Zhang, Jingjing
Zhang, Shizhi
Niu, Chaoqun
Liu, Chen
Du, Jie
Chen, Yong
author_sort Zhang, Jingjing
collection PubMed
description Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M′) as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50–500 nmol/L (R(2) = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive.
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spelling pubmed-62224192018-11-13 A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP Zhang, Jingjing Zhang, Shizhi Niu, Chaoqun Liu, Chen Du, Jie Chen, Yong Molecules Article Herein we described a deoxyribonucleic acid (DNA) calculator for sensitive detection of the determination of adenosine triphosphate (ATP) using gold nanoparticles (GNP) and PicoGreen fluorescence dye as signal transducer, and ATP and single-stranded DNA (DNA-M′) as activators. The calculator-related performances including linearity, reaction time, logic gate, and selectivity were investigated, respectively. The results revealed that this oligonucleotide sensor was highly sensitive and selective. The detection range was 50–500 nmol/L (R(2) = 0.99391) and the detection limit was 46.5 nmol/L. The AND DNA calculator was successfully used for the ATP detection in human urine. Compared with other methods, this DNA calculator has the characteristics of being label-free, non-enzymic, simple, and highly sensitive. MDPI 2018-09-29 /pmc/articles/PMC6222419/ /pubmed/30274237 http://dx.doi.org/10.3390/molecules23102494 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Jingjing
Zhang, Shizhi
Niu, Chaoqun
Liu, Chen
Du, Jie
Chen, Yong
A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP
title A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP
title_full A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP
title_fullStr A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP
title_full_unstemmed A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP
title_short A Label-Free Fluorescent DNA Calculator Based on Gold Nanoparticles for Sensitive Detection of ATP
title_sort label-free fluorescent dna calculator based on gold nanoparticles for sensitive detection of atp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222419/
https://www.ncbi.nlm.nih.gov/pubmed/30274237
http://dx.doi.org/10.3390/molecules23102494
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