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Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3
Tumor suppressor p53-directed apoptosis triggers loss of normal cells, which contributes to the side-effects from anticancer therapies. Thus, small molecules with potential to downregulate the activation of p53 could minimize pathology emerging from anticancer therapies. Acetylation of p53 by the hi...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222455/ https://www.ncbi.nlm.nih.gov/pubmed/30072621 http://dx.doi.org/10.3390/molecules23081930 |
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author | Vincek, Adam S. Patel, Jigneshkumar Jaganathan, Anbalagan Green, Antonia Pierre-Louis, Valerie Arora, Vimal Rehmann, Jill Mezei, Mihaly Zhou, Ming-Ming Ohlmeyer, Michael Mujtaba, Shiraz |
author_facet | Vincek, Adam S. Patel, Jigneshkumar Jaganathan, Anbalagan Green, Antonia Pierre-Louis, Valerie Arora, Vimal Rehmann, Jill Mezei, Mihaly Zhou, Ming-Ming Ohlmeyer, Michael Mujtaba, Shiraz |
author_sort | Vincek, Adam S. |
collection | PubMed |
description | Tumor suppressor p53-directed apoptosis triggers loss of normal cells, which contributes to the side-effects from anticancer therapies. Thus, small molecules with potential to downregulate the activation of p53 could minimize pathology emerging from anticancer therapies. Acetylation of p53 by the histone acetyltransferase (HAT) domain is the hallmark of coactivator CREB-binding protein (CBP) epigenetic function. During genotoxic stress, CBP HAT-mediated acetylation is essential for the activation of p53 to transcriptionally govern target genes, which control cellular responses. Here, we present a small molecule, NiCur, which blocks CBP HAT activity and downregulates p53 activation upon genotoxic stress. Computational modeling reveals that NiCur docks into the active site of CBP HAT. On CDKN1A promoter, the recruitment of p53 as well as RNA Polymerase II and levels of acetylation on histone H3 were diminished by NiCur. Specifically, NiCur reduces the levels of acetylation at lysine 27 on histone H3, which concomitantly increases the levels of trimethylation at lysine 27. Finally, NiCur attenuates p53-directed apoptosis by inhibiting the Caspase 3 activity and cleavage of Poly (ADP-ribose) polymerase (PARP) in normal gastrointestinal epithelial cells. Collectively, NiCur demonstrates the potential to reprogram the chromatin landscape and modulate biological outcomes of CBP-mediated acetylation under normal and disease conditions. |
format | Online Article Text |
id | pubmed-6222455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62224552018-11-13 Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3 Vincek, Adam S. Patel, Jigneshkumar Jaganathan, Anbalagan Green, Antonia Pierre-Louis, Valerie Arora, Vimal Rehmann, Jill Mezei, Mihaly Zhou, Ming-Ming Ohlmeyer, Michael Mujtaba, Shiraz Molecules Article Tumor suppressor p53-directed apoptosis triggers loss of normal cells, which contributes to the side-effects from anticancer therapies. Thus, small molecules with potential to downregulate the activation of p53 could minimize pathology emerging from anticancer therapies. Acetylation of p53 by the histone acetyltransferase (HAT) domain is the hallmark of coactivator CREB-binding protein (CBP) epigenetic function. During genotoxic stress, CBP HAT-mediated acetylation is essential for the activation of p53 to transcriptionally govern target genes, which control cellular responses. Here, we present a small molecule, NiCur, which blocks CBP HAT activity and downregulates p53 activation upon genotoxic stress. Computational modeling reveals that NiCur docks into the active site of CBP HAT. On CDKN1A promoter, the recruitment of p53 as well as RNA Polymerase II and levels of acetylation on histone H3 were diminished by NiCur. Specifically, NiCur reduces the levels of acetylation at lysine 27 on histone H3, which concomitantly increases the levels of trimethylation at lysine 27. Finally, NiCur attenuates p53-directed apoptosis by inhibiting the Caspase 3 activity and cleavage of Poly (ADP-ribose) polymerase (PARP) in normal gastrointestinal epithelial cells. Collectively, NiCur demonstrates the potential to reprogram the chromatin landscape and modulate biological outcomes of CBP-mediated acetylation under normal and disease conditions. MDPI 2018-08-02 /pmc/articles/PMC6222455/ /pubmed/30072621 http://dx.doi.org/10.3390/molecules23081930 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vincek, Adam S. Patel, Jigneshkumar Jaganathan, Anbalagan Green, Antonia Pierre-Louis, Valerie Arora, Vimal Rehmann, Jill Mezei, Mihaly Zhou, Ming-Ming Ohlmeyer, Michael Mujtaba, Shiraz Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3 |
title | Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3 |
title_full | Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3 |
title_fullStr | Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3 |
title_full_unstemmed | Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3 |
title_short | Inhibitor of CBP Histone Acetyltransferase Downregulates p53 Activation and Facilitates Methylation at Lysine 27 on Histone H3 |
title_sort | inhibitor of cbp histone acetyltransferase downregulates p53 activation and facilitates methylation at lysine 27 on histone h3 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222455/ https://www.ncbi.nlm.nih.gov/pubmed/30072621 http://dx.doi.org/10.3390/molecules23081930 |
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