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Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618(T) and Subsequent Lactic Acid Fermentation
Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222552/ https://www.ncbi.nlm.nih.gov/pubmed/30322101 http://dx.doi.org/10.3390/molecules23102626 |
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author | Xie, Yuejiao Hu, Qing Feng, Guodong Jiang, Xu Hu, Jinlong He, Mingxiong Hu, Guoquan Zhao, Shumiao Liang, Yunxiang Ruan, Zhiyong Peng, Nan |
author_facet | Xie, Yuejiao Hu, Qing Feng, Guodong Jiang, Xu Hu, Jinlong He, Mingxiong Hu, Guoquan Zhao, Shumiao Liang, Yunxiang Ruan, Zhiyong Peng, Nan |
author_sort | Xie, Yuejiao |
collection | PubMed |
description | Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount of wastewater, and leads to the loss of solid fiber particles and fermentable sugars. Kurthia huakuii LAM0618(T), a recently identified microorganism, was herein shown to be able to efficiently transform phenolic compounds (syringaldehyde, hydroxybenzaldehyde, and vanillin) into less toxic acids. Taking advantage of these properties, a biodetoxification method was established by inoculating K. huakuii LAM0618(T) into the NH(3)/H(2)O(2)-pretreated unwashed corn stover to degrade phenolic inhibitors and weak acids generated during the pretreatment. Subsequently, 33.47 and 17.91 g/L lactic acid was produced by Bacillus coagulans LA204 at 50 °C through simultaneous saccharification and fermentation (SSF) from 8% (w/w) of NH(3)/H(2)O(2)-pretreated corn stover with or without K. huakuii LAM0618(T)-biodetoxification, indicating biodetoxification significantly increased lactic acid titer and yield. Importantly, using 15% (w/w) of the NH(3)/H(2)O(2)-pretreated K. huakuii LAM0618(T)-biodetoxified corn stover as a substrate through fed-batch simultaneous saccharification and fermentation, high titer and high yield of lactic acid (84.49 g/L and 0.56 g/g corn stover, respectively, with a productivity of 0.88 g/L/h) were produced by Bacillus coagulans LA204. Therefore, this study reported the first study on biodetoxification of alkaline-pretreated lignocellulosic material, and this biodetoxification method could replace water rinsing for removal of phenolic inhibitors and applied in biofuel and biochemical production using the alkaline-pretreated lignocellulosic bioresources. |
format | Online Article Text |
id | pubmed-6222552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62225522018-11-13 Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618(T) and Subsequent Lactic Acid Fermentation Xie, Yuejiao Hu, Qing Feng, Guodong Jiang, Xu Hu, Jinlong He, Mingxiong Hu, Guoquan Zhao, Shumiao Liang, Yunxiang Ruan, Zhiyong Peng, Nan Molecules Article Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount of wastewater, and leads to the loss of solid fiber particles and fermentable sugars. Kurthia huakuii LAM0618(T), a recently identified microorganism, was herein shown to be able to efficiently transform phenolic compounds (syringaldehyde, hydroxybenzaldehyde, and vanillin) into less toxic acids. Taking advantage of these properties, a biodetoxification method was established by inoculating K. huakuii LAM0618(T) into the NH(3)/H(2)O(2)-pretreated unwashed corn stover to degrade phenolic inhibitors and weak acids generated during the pretreatment. Subsequently, 33.47 and 17.91 g/L lactic acid was produced by Bacillus coagulans LA204 at 50 °C through simultaneous saccharification and fermentation (SSF) from 8% (w/w) of NH(3)/H(2)O(2)-pretreated corn stover with or without K. huakuii LAM0618(T)-biodetoxification, indicating biodetoxification significantly increased lactic acid titer and yield. Importantly, using 15% (w/w) of the NH(3)/H(2)O(2)-pretreated K. huakuii LAM0618(T)-biodetoxified corn stover as a substrate through fed-batch simultaneous saccharification and fermentation, high titer and high yield of lactic acid (84.49 g/L and 0.56 g/g corn stover, respectively, with a productivity of 0.88 g/L/h) were produced by Bacillus coagulans LA204. Therefore, this study reported the first study on biodetoxification of alkaline-pretreated lignocellulosic material, and this biodetoxification method could replace water rinsing for removal of phenolic inhibitors and applied in biofuel and biochemical production using the alkaline-pretreated lignocellulosic bioresources. MDPI 2018-10-12 /pmc/articles/PMC6222552/ /pubmed/30322101 http://dx.doi.org/10.3390/molecules23102626 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xie, Yuejiao Hu, Qing Feng, Guodong Jiang, Xu Hu, Jinlong He, Mingxiong Hu, Guoquan Zhao, Shumiao Liang, Yunxiang Ruan, Zhiyong Peng, Nan Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618(T) and Subsequent Lactic Acid Fermentation |
title | Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618(T) and Subsequent Lactic Acid Fermentation |
title_full | Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618(T) and Subsequent Lactic Acid Fermentation |
title_fullStr | Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618(T) and Subsequent Lactic Acid Fermentation |
title_full_unstemmed | Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618(T) and Subsequent Lactic Acid Fermentation |
title_short | Biodetoxification of Phenolic Inhibitors from Lignocellulose Pretreatment using Kurthia huakuii LAM0618(T) and Subsequent Lactic Acid Fermentation |
title_sort | biodetoxification of phenolic inhibitors from lignocellulose pretreatment using kurthia huakuii lam0618(t) and subsequent lactic acid fermentation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222552/ https://www.ncbi.nlm.nih.gov/pubmed/30322101 http://dx.doi.org/10.3390/molecules23102626 |
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