An Efficient Genome Editing Strategy To Generate Putative Null Mutants in Caenorhabditis elegans Using CRISPR/Cas9

Null mutants are essential for analyzing gene function. Here, we describe a simple and efficient method to generate Caenorhabditis elegans null mutants using CRISPR/Cas9 and short single stranded DNA oligo repair templates to insert a universal 43-nucleotide-long knock-in cassette (STOP-IN) into the...

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Detalles Bibliográficos
Autores principales: Wang, Han, Park, Heenam, Liu, Jonathan, Sternberg, Paul W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2018
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222585/
https://www.ncbi.nlm.nih.gov/pubmed/30224336
http://dx.doi.org/10.1534/g3.118.200662

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