Cargando…
Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor
Ganoderma lucidum, a famous medicinal mushroom used worldwide, is a rich source of triterpenoids which, together with polysaccharides, are believed to be the main effective constituents of G. lucidum. With the increase of market demand, the wild resource is facing serious limitations, and the qualit...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222601/ https://www.ncbi.nlm.nih.gov/pubmed/30297630 http://dx.doi.org/10.3390/molecules23102563 |
_version_ | 1783369243931181056 |
---|---|
author | Hu, Gaosheng Zhai, Manhuayun Niu, Rong Xu, Xiaoqiang Liu, Qian Jia, Jingming |
author_facet | Hu, Gaosheng Zhai, Manhuayun Niu, Rong Xu, Xiaoqiang Liu, Qian Jia, Jingming |
author_sort | Hu, Gaosheng |
collection | PubMed |
description | Ganoderma lucidum, a famous medicinal mushroom used worldwide, is a rich source of triterpenoids which, together with polysaccharides, are believed to be the main effective constituents of G. lucidum. With the increase of market demand, the wild resource is facing serious limitations, and the quality of cultivated fruiting bodies can be seriously affected by the availability of wood resources and by cultivation management practices. In the present study, we aimed to develop an alternative way to produce useful triterpenoids from G. lucidum. We cultured the strain using a two-stage liquid culture strategy and investigated the effects of nitrogen limitation, carbon supply, static culture volume and air supply in the static culture stage on the accumulation of five triterpenoids (GA-P, GA-Q, GA-T, GA-S, GA-R). Our results showed that, under optimized condition, the total yield of the five triterpenoids reached 963 mg/L (as determined by HPLC). Among the five triterpenoids, GA-T accounted for about 75% of the total yield. Besides, a bioreactor suitable for fungal liquid static culture with a 10 L extensible plastic bag shaped culture unit was designed and in which the maximum total yield of the five GAs reached 856.8 mg/L, and the GAs content reached 5.99%. Our results demonstrate the potential of industrial application of G. lucidum culture for the production of triterpenoids, especially GA-T. Air supply significantly improved the accumulation of triterpenoids, and this will provide important clues to understand why more triterpenoids are produced in the mycelia mat under static liquid culture conditions. |
format | Online Article Text |
id | pubmed-6222601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62226012018-11-13 Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor Hu, Gaosheng Zhai, Manhuayun Niu, Rong Xu, Xiaoqiang Liu, Qian Jia, Jingming Molecules Article Ganoderma lucidum, a famous medicinal mushroom used worldwide, is a rich source of triterpenoids which, together with polysaccharides, are believed to be the main effective constituents of G. lucidum. With the increase of market demand, the wild resource is facing serious limitations, and the quality of cultivated fruiting bodies can be seriously affected by the availability of wood resources and by cultivation management practices. In the present study, we aimed to develop an alternative way to produce useful triterpenoids from G. lucidum. We cultured the strain using a two-stage liquid culture strategy and investigated the effects of nitrogen limitation, carbon supply, static culture volume and air supply in the static culture stage on the accumulation of five triterpenoids (GA-P, GA-Q, GA-T, GA-S, GA-R). Our results showed that, under optimized condition, the total yield of the five triterpenoids reached 963 mg/L (as determined by HPLC). Among the five triterpenoids, GA-T accounted for about 75% of the total yield. Besides, a bioreactor suitable for fungal liquid static culture with a 10 L extensible plastic bag shaped culture unit was designed and in which the maximum total yield of the five GAs reached 856.8 mg/L, and the GAs content reached 5.99%. Our results demonstrate the potential of industrial application of G. lucidum culture for the production of triterpenoids, especially GA-T. Air supply significantly improved the accumulation of triterpenoids, and this will provide important clues to understand why more triterpenoids are produced in the mycelia mat under static liquid culture conditions. MDPI 2018-10-08 /pmc/articles/PMC6222601/ /pubmed/30297630 http://dx.doi.org/10.3390/molecules23102563 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hu, Gaosheng Zhai, Manhuayun Niu, Rong Xu, Xiaoqiang Liu, Qian Jia, Jingming Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor |
title | Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor |
title_full | Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor |
title_fullStr | Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor |
title_full_unstemmed | Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor |
title_short | Optimization of Culture Condition for Ganoderic Acid Production in Ganoderma lucidum Liquid Static Culture and Design of a Suitable Bioreactor |
title_sort | optimization of culture condition for ganoderic acid production in ganoderma lucidum liquid static culture and design of a suitable bioreactor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222601/ https://www.ncbi.nlm.nih.gov/pubmed/30297630 http://dx.doi.org/10.3390/molecules23102563 |
work_keys_str_mv | AT hugaosheng optimizationofcultureconditionforganodericacidproductioninganodermalucidumliquidstaticcultureanddesignofasuitablebioreactor AT zhaimanhuayun optimizationofcultureconditionforganodericacidproductioninganodermalucidumliquidstaticcultureanddesignofasuitablebioreactor AT niurong optimizationofcultureconditionforganodericacidproductioninganodermalucidumliquidstaticcultureanddesignofasuitablebioreactor AT xuxiaoqiang optimizationofcultureconditionforganodericacidproductioninganodermalucidumliquidstaticcultureanddesignofasuitablebioreactor AT liuqian optimizationofcultureconditionforganodericacidproductioninganodermalucidumliquidstaticcultureanddesignofasuitablebioreactor AT jiajingming optimizationofcultureconditionforganodericacidproductioninganodermalucidumliquidstaticcultureanddesignofasuitablebioreactor |