Cargando…

Comparison of Two Methods for the Determination of Selected Pesticides in Honey and Honeybee Samples

Developed and validated analytical methods for the determination of a wide spectrum of pesticide residues in honey and honeybee samples after the modification of QuEChERS extraction in combination with gas chromatography–tandem quadrupole mass spectrometry (GC-MS/MS) and liquid chromatography–tandem...

Descripción completa

Detalles Bibliográficos
Autores principales: Bargańska, Żaneta, Konieczka, Piotr, Namieśnik, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222677/
https://www.ncbi.nlm.nih.gov/pubmed/30304845
http://dx.doi.org/10.3390/molecules23102582
_version_ 1783369262099857408
author Bargańska, Żaneta
Konieczka, Piotr
Namieśnik, Jacek
author_facet Bargańska, Żaneta
Konieczka, Piotr
Namieśnik, Jacek
author_sort Bargańska, Żaneta
collection PubMed
description Developed and validated analytical methods for the determination of a wide spectrum of pesticide residues in honey and honeybee samples after the modification of QuEChERS extraction in combination with gas chromatography–tandem quadrupole mass spectrometry (GC-MS/MS) and liquid chromatography–tandem quadrupole mass spectrometry (LC-MS/MS) were discussed and compared. The developed methods were evaluated regarding the utilized equipment and reagents using Eco-Scale and compared in terms of extraction time, accuracy, precision, sensitivity and versatility, with similar procedures. The results proved that the QuEChERS protocol in combination with LC and GC techniques fulfills the requirements of green analytical chemistry, so it can be used as a tool in environmental monitoring. The recovery was 85–116% for honey and 85.5–103.5% for honeybee samples. The developed methods were successfully applied in monitoring real samples collected from three districts of Pomerania in Poland. Analysis of real samples revealed the presence of the following pesticides: bifenthrin, fenpyroximate, methidathione, spinosad, thiamethoxam, triazophos, metconazole and cypermethrin at levels higher than the MRLs established by the EU.
format Online
Article
Text
id pubmed-6222677
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62226772018-11-13 Comparison of Two Methods for the Determination of Selected Pesticides in Honey and Honeybee Samples Bargańska, Żaneta Konieczka, Piotr Namieśnik, Jacek Molecules Article Developed and validated analytical methods for the determination of a wide spectrum of pesticide residues in honey and honeybee samples after the modification of QuEChERS extraction in combination with gas chromatography–tandem quadrupole mass spectrometry (GC-MS/MS) and liquid chromatography–tandem quadrupole mass spectrometry (LC-MS/MS) were discussed and compared. The developed methods were evaluated regarding the utilized equipment and reagents using Eco-Scale and compared in terms of extraction time, accuracy, precision, sensitivity and versatility, with similar procedures. The results proved that the QuEChERS protocol in combination with LC and GC techniques fulfills the requirements of green analytical chemistry, so it can be used as a tool in environmental monitoring. The recovery was 85–116% for honey and 85.5–103.5% for honeybee samples. The developed methods were successfully applied in monitoring real samples collected from three districts of Pomerania in Poland. Analysis of real samples revealed the presence of the following pesticides: bifenthrin, fenpyroximate, methidathione, spinosad, thiamethoxam, triazophos, metconazole and cypermethrin at levels higher than the MRLs established by the EU. MDPI 2018-10-09 /pmc/articles/PMC6222677/ /pubmed/30304845 http://dx.doi.org/10.3390/molecules23102582 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bargańska, Żaneta
Konieczka, Piotr
Namieśnik, Jacek
Comparison of Two Methods for the Determination of Selected Pesticides in Honey and Honeybee Samples
title Comparison of Two Methods for the Determination of Selected Pesticides in Honey and Honeybee Samples
title_full Comparison of Two Methods for the Determination of Selected Pesticides in Honey and Honeybee Samples
title_fullStr Comparison of Two Methods for the Determination of Selected Pesticides in Honey and Honeybee Samples
title_full_unstemmed Comparison of Two Methods for the Determination of Selected Pesticides in Honey and Honeybee Samples
title_short Comparison of Two Methods for the Determination of Selected Pesticides in Honey and Honeybee Samples
title_sort comparison of two methods for the determination of selected pesticides in honey and honeybee samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222677/
https://www.ncbi.nlm.nih.gov/pubmed/30304845
http://dx.doi.org/10.3390/molecules23102582
work_keys_str_mv AT barganskazaneta comparisonoftwomethodsforthedeterminationofselectedpesticidesinhoneyandhoneybeesamples
AT konieczkapiotr comparisonoftwomethodsforthedeterminationofselectedpesticidesinhoneyandhoneybeesamples
AT namiesnikjacek comparisonoftwomethodsforthedeterminationofselectedpesticidesinhoneyandhoneybeesamples