Cargando…

Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula

Human milk oligosaccharides (HMO) are the third most abundant solid component of human milk. It is likely that they are responsible for at least some of the benefits experienced by breast-fed infants. Until recently HMO were absent from infant formula, but 2′-fucosyllactose (2′-FL) and lacto-N-neote...

Descripción completa

Detalles Bibliográficos
Autores principales: Austin, Sean, Cuany, Denis, Michaud, Julien, Diehl, Bernd, Casado, Begoña
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222730/
https://www.ncbi.nlm.nih.gov/pubmed/30332767
http://dx.doi.org/10.3390/molecules23102650
_version_ 1783369274669137920
author Austin, Sean
Cuany, Denis
Michaud, Julien
Diehl, Bernd
Casado, Begoña
author_facet Austin, Sean
Cuany, Denis
Michaud, Julien
Diehl, Bernd
Casado, Begoña
author_sort Austin, Sean
collection PubMed
description Human milk oligosaccharides (HMO) are the third most abundant solid component of human milk. It is likely that they are responsible for at least some of the benefits experienced by breast-fed infants. Until recently HMO were absent from infant formula, but 2′-fucosyllactose (2′-FL) and lacto-N-neoteraose (LNnT) have recently become available as ingredients. The development of formula containing these HMO and the quality control of such formula require suitable methods for the accurate determination of the HMO. We developed two different approaches for analysis of 2′-FL and LNnT in formula; high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-FLD). In lab trials using blank formula spiked with the two oligosaccharides, both approaches worked well with recoveries of 94–111% (HPAEC-PAD) and 94–104% (HILIC-FLD) and RSD (iR) of 2.1–7.9% (HPAEC-PAD) and 2.0–7.4% (HILIC-FLD). However, when applied to products produced in a pilot plant, the HPAEC-PAD approach sometimes delivered results below those expected from the addition rate of the ingredients. We hypothesize that the oligosaccharides interact with the formula matrix during the production process and, during sample preparation for HPAEC-PAD those interactions have not been broken. The conditions required for labeling the HMO for detection by the FLD apparently disrupt those interactions, and result in improved recoveries. It is likely that both analytical approaches are appropriate if a suitable extraction process is used to recover the HMO.
format Online
Article
Text
id pubmed-6222730
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62227302018-11-13 Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula Austin, Sean Cuany, Denis Michaud, Julien Diehl, Bernd Casado, Begoña Molecules Article Human milk oligosaccharides (HMO) are the third most abundant solid component of human milk. It is likely that they are responsible for at least some of the benefits experienced by breast-fed infants. Until recently HMO were absent from infant formula, but 2′-fucosyllactose (2′-FL) and lacto-N-neoteraose (LNnT) have recently become available as ingredients. The development of formula containing these HMO and the quality control of such formula require suitable methods for the accurate determination of the HMO. We developed two different approaches for analysis of 2′-FL and LNnT in formula; high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-FLD). In lab trials using blank formula spiked with the two oligosaccharides, both approaches worked well with recoveries of 94–111% (HPAEC-PAD) and 94–104% (HILIC-FLD) and RSD (iR) of 2.1–7.9% (HPAEC-PAD) and 2.0–7.4% (HILIC-FLD). However, when applied to products produced in a pilot plant, the HPAEC-PAD approach sometimes delivered results below those expected from the addition rate of the ingredients. We hypothesize that the oligosaccharides interact with the formula matrix during the production process and, during sample preparation for HPAEC-PAD those interactions have not been broken. The conditions required for labeling the HMO for detection by the FLD apparently disrupt those interactions, and result in improved recoveries. It is likely that both analytical approaches are appropriate if a suitable extraction process is used to recover the HMO. MDPI 2018-10-16 /pmc/articles/PMC6222730/ /pubmed/30332767 http://dx.doi.org/10.3390/molecules23102650 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Austin, Sean
Cuany, Denis
Michaud, Julien
Diehl, Bernd
Casado, Begoña
Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula
title Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula
title_full Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula
title_fullStr Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula
title_full_unstemmed Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula
title_short Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula
title_sort determination of 2′-fucosyllactose and lacto-n-neotetraose in infant formula
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222730/
https://www.ncbi.nlm.nih.gov/pubmed/30332767
http://dx.doi.org/10.3390/molecules23102650
work_keys_str_mv AT austinsean determinationof2fucosyllactoseandlactonneotetraoseininfantformula
AT cuanydenis determinationof2fucosyllactoseandlactonneotetraoseininfantformula
AT michaudjulien determinationof2fucosyllactoseandlactonneotetraoseininfantformula
AT diehlbernd determinationof2fucosyllactoseandlactonneotetraoseininfantformula
AT casadobegona determinationof2fucosyllactoseandlactonneotetraoseininfantformula