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Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula
Human milk oligosaccharides (HMO) are the third most abundant solid component of human milk. It is likely that they are responsible for at least some of the benefits experienced by breast-fed infants. Until recently HMO were absent from infant formula, but 2′-fucosyllactose (2′-FL) and lacto-N-neote...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222730/ https://www.ncbi.nlm.nih.gov/pubmed/30332767 http://dx.doi.org/10.3390/molecules23102650 |
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author | Austin, Sean Cuany, Denis Michaud, Julien Diehl, Bernd Casado, Begoña |
author_facet | Austin, Sean Cuany, Denis Michaud, Julien Diehl, Bernd Casado, Begoña |
author_sort | Austin, Sean |
collection | PubMed |
description | Human milk oligosaccharides (HMO) are the third most abundant solid component of human milk. It is likely that they are responsible for at least some of the benefits experienced by breast-fed infants. Until recently HMO were absent from infant formula, but 2′-fucosyllactose (2′-FL) and lacto-N-neoteraose (LNnT) have recently become available as ingredients. The development of formula containing these HMO and the quality control of such formula require suitable methods for the accurate determination of the HMO. We developed two different approaches for analysis of 2′-FL and LNnT in formula; high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-FLD). In lab trials using blank formula spiked with the two oligosaccharides, both approaches worked well with recoveries of 94–111% (HPAEC-PAD) and 94–104% (HILIC-FLD) and RSD (iR) of 2.1–7.9% (HPAEC-PAD) and 2.0–7.4% (HILIC-FLD). However, when applied to products produced in a pilot plant, the HPAEC-PAD approach sometimes delivered results below those expected from the addition rate of the ingredients. We hypothesize that the oligosaccharides interact with the formula matrix during the production process and, during sample preparation for HPAEC-PAD those interactions have not been broken. The conditions required for labeling the HMO for detection by the FLD apparently disrupt those interactions, and result in improved recoveries. It is likely that both analytical approaches are appropriate if a suitable extraction process is used to recover the HMO. |
format | Online Article Text |
id | pubmed-6222730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62227302018-11-13 Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula Austin, Sean Cuany, Denis Michaud, Julien Diehl, Bernd Casado, Begoña Molecules Article Human milk oligosaccharides (HMO) are the third most abundant solid component of human milk. It is likely that they are responsible for at least some of the benefits experienced by breast-fed infants. Until recently HMO were absent from infant formula, but 2′-fucosyllactose (2′-FL) and lacto-N-neoteraose (LNnT) have recently become available as ingredients. The development of formula containing these HMO and the quality control of such formula require suitable methods for the accurate determination of the HMO. We developed two different approaches for analysis of 2′-FL and LNnT in formula; high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-FLD). In lab trials using blank formula spiked with the two oligosaccharides, both approaches worked well with recoveries of 94–111% (HPAEC-PAD) and 94–104% (HILIC-FLD) and RSD (iR) of 2.1–7.9% (HPAEC-PAD) and 2.0–7.4% (HILIC-FLD). However, when applied to products produced in a pilot plant, the HPAEC-PAD approach sometimes delivered results below those expected from the addition rate of the ingredients. We hypothesize that the oligosaccharides interact with the formula matrix during the production process and, during sample preparation for HPAEC-PAD those interactions have not been broken. The conditions required for labeling the HMO for detection by the FLD apparently disrupt those interactions, and result in improved recoveries. It is likely that both analytical approaches are appropriate if a suitable extraction process is used to recover the HMO. MDPI 2018-10-16 /pmc/articles/PMC6222730/ /pubmed/30332767 http://dx.doi.org/10.3390/molecules23102650 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Austin, Sean Cuany, Denis Michaud, Julien Diehl, Bernd Casado, Begoña Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula |
title | Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula |
title_full | Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula |
title_fullStr | Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula |
title_full_unstemmed | Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula |
title_short | Determination of 2′-Fucosyllactose and Lacto-N-neotetraose in Infant Formula |
title_sort | determination of 2′-fucosyllactose and lacto-n-neotetraose in infant formula |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222730/ https://www.ncbi.nlm.nih.gov/pubmed/30332767 http://dx.doi.org/10.3390/molecules23102650 |
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