2′-O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA

In contrast to Z-DNA that was stabilized and well-studied for its structure by chemical approaches, the stabilization and structural study of Z-RNA remains a challenge. In this study, we developed a Z-form RNA stabilizer m(8)Gm, and demonstrated that incorporation of m(8)Gm into RNA can markedly sta...

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Autores principales: Balasubramaniyam, Thananjeyan, Ishizuka, Takumi, Xiao, Chao-Da, Bao, Hong-Liang, Xu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222775/
https://www.ncbi.nlm.nih.gov/pubmed/30304782
http://dx.doi.org/10.3390/molecules23102572
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author Balasubramaniyam, Thananjeyan
Ishizuka, Takumi
Xiao, Chao-Da
Bao, Hong-Liang
Xu, Yan
author_facet Balasubramaniyam, Thananjeyan
Ishizuka, Takumi
Xiao, Chao-Da
Bao, Hong-Liang
Xu, Yan
author_sort Balasubramaniyam, Thananjeyan
collection PubMed
description In contrast to Z-DNA that was stabilized and well-studied for its structure by chemical approaches, the stabilization and structural study of Z-RNA remains a challenge. In this study, we developed a Z-form RNA stabilizer m(8)Gm, and demonstrated that incorporation of m(8)Gm into RNA can markedly stabilize the Z-RNA at low salt conditions. Using the m(8)Gm-contained Z-RNA, we determined the structure of Z-RNA and investigated the interaction of protein and Z-RNA.
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spelling pubmed-62227752018-11-13 2′-O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA Balasubramaniyam, Thananjeyan Ishizuka, Takumi Xiao, Chao-Da Bao, Hong-Liang Xu, Yan Molecules Article In contrast to Z-DNA that was stabilized and well-studied for its structure by chemical approaches, the stabilization and structural study of Z-RNA remains a challenge. In this study, we developed a Z-form RNA stabilizer m(8)Gm, and demonstrated that incorporation of m(8)Gm into RNA can markedly stabilize the Z-RNA at low salt conditions. Using the m(8)Gm-contained Z-RNA, we determined the structure of Z-RNA and investigated the interaction of protein and Z-RNA. MDPI 2018-10-09 /pmc/articles/PMC6222775/ /pubmed/30304782 http://dx.doi.org/10.3390/molecules23102572 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Balasubramaniyam, Thananjeyan
Ishizuka, Takumi
Xiao, Chao-Da
Bao, Hong-Liang
Xu, Yan
2′-O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA
title 2′-O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA
title_full 2′-O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA
title_fullStr 2′-O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA
title_full_unstemmed 2′-O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA
title_short 2′-O-Methyl-8-methylguanosine as a Z-Form RNA Stabilizer for Structural and Functional Study of Z-RNA
title_sort 2′-o-methyl-8-methylguanosine as a z-form rna stabilizer for structural and functional study of z-rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222775/
https://www.ncbi.nlm.nih.gov/pubmed/30304782
http://dx.doi.org/10.3390/molecules23102572
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