Cargando…

Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System

In this research, fifteen carbamate pesticide residues were systematically analyzed by ultra-high performance liquid chromatography–quadrupole-linear ion trap mass spectrometry on a QTRAP 5500 system in both multiple reaction monitoring (MRM) and enhanced product ion (EPI) scan modes. The carbamate...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Ying, Guan, Jian, Gao, Weiwei, Lv, Shencong, Ge, Miaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222809/
https://www.ncbi.nlm.nih.gov/pubmed/30274254
http://dx.doi.org/10.3390/molecules23102496
_version_ 1783369293473251328
author Zhou, Ying
Guan, Jian
Gao, Weiwei
Lv, Shencong
Ge, Miaohua
author_facet Zhou, Ying
Guan, Jian
Gao, Weiwei
Lv, Shencong
Ge, Miaohua
author_sort Zhou, Ying
collection PubMed
description In this research, fifteen carbamate pesticide residues were systematically analyzed by ultra-high performance liquid chromatography–quadrupole-linear ion trap mass spectrometry on a QTRAP 5500 system in both multiple reaction monitoring (MRM) and enhanced product ion (EPI) scan modes. The carbamate pesticide residues were extracted from a variety of samples by QuEChERS method and separated by a popular reverse phase column (Waters BEH C18). Except for the current conformation criteria including selected ion pairs, retention time and relative intensities from MRM scan mode, the presence of carbamate pesticide residues in diverse samples, especially some doubtful cases, could also be confirmed by the matching of carbamate pesticide spectra via EPI scan mode. Moreover, the fragmentation routes of fifteen carbamates were firstly explained based on the mass spectra obtained by a QTRAP system; the characteristic fragment ion from a neutral loss of CH(3)NCO (−57 Da) could be observed. The limits of detection and quantification for fifteen carbamates were 0.2–2.0 μg kg(−1) and 0.5–5.0 μg kg(−1), respectively. For the intra- (n = 3) and inter-day (n = 15) precisions, the recoveries of fifteen carbamates from spiked samples ranged from 88.1% to 118.4%, and the coefficients of variation (CVs) were all below 10%. The method was applied to pesticide residues detection in fruit, vegetable and green tea samples taken from local markets, in which carbamates were extensively detected but all below the standard of maximum residue limit.
format Online
Article
Text
id pubmed-6222809
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62228092018-11-13 Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System Zhou, Ying Guan, Jian Gao, Weiwei Lv, Shencong Ge, Miaohua Molecules Article In this research, fifteen carbamate pesticide residues were systematically analyzed by ultra-high performance liquid chromatography–quadrupole-linear ion trap mass spectrometry on a QTRAP 5500 system in both multiple reaction monitoring (MRM) and enhanced product ion (EPI) scan modes. The carbamate pesticide residues were extracted from a variety of samples by QuEChERS method and separated by a popular reverse phase column (Waters BEH C18). Except for the current conformation criteria including selected ion pairs, retention time and relative intensities from MRM scan mode, the presence of carbamate pesticide residues in diverse samples, especially some doubtful cases, could also be confirmed by the matching of carbamate pesticide spectra via EPI scan mode. Moreover, the fragmentation routes of fifteen carbamates were firstly explained based on the mass spectra obtained by a QTRAP system; the characteristic fragment ion from a neutral loss of CH(3)NCO (−57 Da) could be observed. The limits of detection and quantification for fifteen carbamates were 0.2–2.0 μg kg(−1) and 0.5–5.0 μg kg(−1), respectively. For the intra- (n = 3) and inter-day (n = 15) precisions, the recoveries of fifteen carbamates from spiked samples ranged from 88.1% to 118.4%, and the coefficients of variation (CVs) were all below 10%. The method was applied to pesticide residues detection in fruit, vegetable and green tea samples taken from local markets, in which carbamates were extensively detected but all below the standard of maximum residue limit. MDPI 2018-09-29 /pmc/articles/PMC6222809/ /pubmed/30274254 http://dx.doi.org/10.3390/molecules23102496 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Ying
Guan, Jian
Gao, Weiwei
Lv, Shencong
Ge, Miaohua
Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System
title Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System
title_full Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System
title_fullStr Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System
title_full_unstemmed Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System
title_short Quantification and Confirmation of Fifteen Carbamate Pesticide Residues by Multiple Reaction Monitoring and Enhanced Product Ion Scan Modes via LC-MS/MS QTRAP System
title_sort quantification and confirmation of fifteen carbamate pesticide residues by multiple reaction monitoring and enhanced product ion scan modes via lc-ms/ms qtrap system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222809/
https://www.ncbi.nlm.nih.gov/pubmed/30274254
http://dx.doi.org/10.3390/molecules23102496
work_keys_str_mv AT zhouying quantificationandconfirmationoffifteencarbamatepesticideresiduesbymultiplereactionmonitoringandenhancedproductionscanmodesvialcmsmsqtrapsystem
AT guanjian quantificationandconfirmationoffifteencarbamatepesticideresiduesbymultiplereactionmonitoringandenhancedproductionscanmodesvialcmsmsqtrapsystem
AT gaoweiwei quantificationandconfirmationoffifteencarbamatepesticideresiduesbymultiplereactionmonitoringandenhancedproductionscanmodesvialcmsmsqtrapsystem
AT lvshencong quantificationandconfirmationoffifteencarbamatepesticideresiduesbymultiplereactionmonitoringandenhancedproductionscanmodesvialcmsmsqtrapsystem
AT gemiaohua quantificationandconfirmationoffifteencarbamatepesticideresiduesbymultiplereactionmonitoringandenhancedproductionscanmodesvialcmsmsqtrapsystem