Cargando…

Isolation of Tricin as a Xanthine Oxidase Inhibitor from Sweet White Clover (Melilotus albus) and Its Distribution in Selected Gramineae Species

Xanthine oxidase, an enzyme present in significant levels in the intestine and liver, metabolizes hypoxanthine to xanthine and xanthine to uric acid in the purine catabolic pathway. An inhibitory compound acting against xanthine oxidase was isolated from sweet white clover (Melilotus albus) by bioas...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Xiao-Xiao, Sun, Shi-Wei, Yuan, Wen-Jing, Gao, Hua, Si, Yue-Yue, Liu, Kun, Zhang, Shuang, Liu, Yang, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222886/
https://www.ncbi.nlm.nih.gov/pubmed/30360380
http://dx.doi.org/10.3390/molecules23102719
_version_ 1783369311652413440
author Liu, Xiao-Xiao
Sun, Shi-Wei
Yuan, Wen-Jing
Gao, Hua
Si, Yue-Yue
Liu, Kun
Zhang, Shuang
Liu, Yang
Wang, Wei
author_facet Liu, Xiao-Xiao
Sun, Shi-Wei
Yuan, Wen-Jing
Gao, Hua
Si, Yue-Yue
Liu, Kun
Zhang, Shuang
Liu, Yang
Wang, Wei
author_sort Liu, Xiao-Xiao
collection PubMed
description Xanthine oxidase, an enzyme present in significant levels in the intestine and liver, metabolizes hypoxanthine to xanthine and xanthine to uric acid in the purine catabolic pathway. An inhibitory compound acting against xanthine oxidase was isolated from sweet white clover (Melilotus albus) by bioassay and high-performance liquid chromatography guided separation. It was identified as tricin by spectroscopic analysis. Tricin possessed a potent xanthine oxidase inhibitory activity with an IC(50) value of 4.13 μM. Further inhibition kinetics data indicated it to be a mixed-type inhibitor and K(i) and K(I) values were determined to be 0.47 μM and 4.41 μM. To find a rich source of tricin, the distribution of tricin in seven different tissues from four Gramineae species was investigated by high-performance liquid chromatography analysis. The highest amount (1925.05 mg/kg dry materials) was found in the straw of wheat, which is considered as a potentially valuable source of natural tricin.
format Online
Article
Text
id pubmed-6222886
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62228862018-11-13 Isolation of Tricin as a Xanthine Oxidase Inhibitor from Sweet White Clover (Melilotus albus) and Its Distribution in Selected Gramineae Species Liu, Xiao-Xiao Sun, Shi-Wei Yuan, Wen-Jing Gao, Hua Si, Yue-Yue Liu, Kun Zhang, Shuang Liu, Yang Wang, Wei Molecules Article Xanthine oxidase, an enzyme present in significant levels in the intestine and liver, metabolizes hypoxanthine to xanthine and xanthine to uric acid in the purine catabolic pathway. An inhibitory compound acting against xanthine oxidase was isolated from sweet white clover (Melilotus albus) by bioassay and high-performance liquid chromatography guided separation. It was identified as tricin by spectroscopic analysis. Tricin possessed a potent xanthine oxidase inhibitory activity with an IC(50) value of 4.13 μM. Further inhibition kinetics data indicated it to be a mixed-type inhibitor and K(i) and K(I) values were determined to be 0.47 μM and 4.41 μM. To find a rich source of tricin, the distribution of tricin in seven different tissues from four Gramineae species was investigated by high-performance liquid chromatography analysis. The highest amount (1925.05 mg/kg dry materials) was found in the straw of wheat, which is considered as a potentially valuable source of natural tricin. MDPI 2018-10-22 /pmc/articles/PMC6222886/ /pubmed/30360380 http://dx.doi.org/10.3390/molecules23102719 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xiao-Xiao
Sun, Shi-Wei
Yuan, Wen-Jing
Gao, Hua
Si, Yue-Yue
Liu, Kun
Zhang, Shuang
Liu, Yang
Wang, Wei
Isolation of Tricin as a Xanthine Oxidase Inhibitor from Sweet White Clover (Melilotus albus) and Its Distribution in Selected Gramineae Species
title Isolation of Tricin as a Xanthine Oxidase Inhibitor from Sweet White Clover (Melilotus albus) and Its Distribution in Selected Gramineae Species
title_full Isolation of Tricin as a Xanthine Oxidase Inhibitor from Sweet White Clover (Melilotus albus) and Its Distribution in Selected Gramineae Species
title_fullStr Isolation of Tricin as a Xanthine Oxidase Inhibitor from Sweet White Clover (Melilotus albus) and Its Distribution in Selected Gramineae Species
title_full_unstemmed Isolation of Tricin as a Xanthine Oxidase Inhibitor from Sweet White Clover (Melilotus albus) and Its Distribution in Selected Gramineae Species
title_short Isolation of Tricin as a Xanthine Oxidase Inhibitor from Sweet White Clover (Melilotus albus) and Its Distribution in Selected Gramineae Species
title_sort isolation of tricin as a xanthine oxidase inhibitor from sweet white clover (melilotus albus) and its distribution in selected gramineae species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6222886/
https://www.ncbi.nlm.nih.gov/pubmed/30360380
http://dx.doi.org/10.3390/molecules23102719
work_keys_str_mv AT liuxiaoxiao isolationoftricinasaxanthineoxidaseinhibitorfromsweetwhiteclovermelilotusalbusanditsdistributioninselectedgramineaespecies
AT sunshiwei isolationoftricinasaxanthineoxidaseinhibitorfromsweetwhiteclovermelilotusalbusanditsdistributioninselectedgramineaespecies
AT yuanwenjing isolationoftricinasaxanthineoxidaseinhibitorfromsweetwhiteclovermelilotusalbusanditsdistributioninselectedgramineaespecies
AT gaohua isolationoftricinasaxanthineoxidaseinhibitorfromsweetwhiteclovermelilotusalbusanditsdistributioninselectedgramineaespecies
AT siyueyue isolationoftricinasaxanthineoxidaseinhibitorfromsweetwhiteclovermelilotusalbusanditsdistributioninselectedgramineaespecies
AT liukun isolationoftricinasaxanthineoxidaseinhibitorfromsweetwhiteclovermelilotusalbusanditsdistributioninselectedgramineaespecies
AT zhangshuang isolationoftricinasaxanthineoxidaseinhibitorfromsweetwhiteclovermelilotusalbusanditsdistributioninselectedgramineaespecies
AT liuyang isolationoftricinasaxanthineoxidaseinhibitorfromsweetwhiteclovermelilotusalbusanditsdistributioninselectedgramineaespecies
AT wangwei isolationoftricinasaxanthineoxidaseinhibitorfromsweetwhiteclovermelilotusalbusanditsdistributioninselectedgramineaespecies