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Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes

Fine regulation of exocytosis and endocytosis plays a basic role in pollen tube growth. Excess plasma membrane secreted during pollen tube elongation is known to be retrieved by endocytosis and partially reused in secretory pathways through the Golgi apparatus. Dissection of endocytosis has enabled...

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Autores principales: Onelli, Elisabetta, Scali, Monica, Caccianiga, Marco, Stroppa, Nadia, Morandini, Piero, Pavesi, Giulio, Moscatelli, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223213/
https://www.ncbi.nlm.nih.gov/pubmed/30381363
http://dx.doi.org/10.1098/rsob.180078
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author Onelli, Elisabetta
Scali, Monica
Caccianiga, Marco
Stroppa, Nadia
Morandini, Piero
Pavesi, Giulio
Moscatelli, Alessandra
author_facet Onelli, Elisabetta
Scali, Monica
Caccianiga, Marco
Stroppa, Nadia
Morandini, Piero
Pavesi, Giulio
Moscatelli, Alessandra
author_sort Onelli, Elisabetta
collection PubMed
description Fine regulation of exocytosis and endocytosis plays a basic role in pollen tube growth. Excess plasma membrane secreted during pollen tube elongation is known to be retrieved by endocytosis and partially reused in secretory pathways through the Golgi apparatus. Dissection of endocytosis has enabled distinct degradation pathways to be identified in tobacco pollen tubes and has shown that microtubules influence the transport of plasma membrane internalized in the tip region to vacuoles. Here, we used different drugs affecting the polymerization state of microtubules together with SYP21, a marker of prevacuolar compartments, to characterize trafficking of prevacuolar compartments in Nicotiana tabacum pollen tubes. Ultrastructural and biochemical analysis showed that microtubules bind SYP21-positive microsomes. Transient transformation of pollen tubes with LAT52-YFP-SYP21 revealed that microtubules play a key role in the delivery of prevacuolar compartments to tubular vacuoles.
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spelling pubmed-62232132018-11-20 Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes Onelli, Elisabetta Scali, Monica Caccianiga, Marco Stroppa, Nadia Morandini, Piero Pavesi, Giulio Moscatelli, Alessandra Open Biol Research Fine regulation of exocytosis and endocytosis plays a basic role in pollen tube growth. Excess plasma membrane secreted during pollen tube elongation is known to be retrieved by endocytosis and partially reused in secretory pathways through the Golgi apparatus. Dissection of endocytosis has enabled distinct degradation pathways to be identified in tobacco pollen tubes and has shown that microtubules influence the transport of plasma membrane internalized in the tip region to vacuoles. Here, we used different drugs affecting the polymerization state of microtubules together with SYP21, a marker of prevacuolar compartments, to characterize trafficking of prevacuolar compartments in Nicotiana tabacum pollen tubes. Ultrastructural and biochemical analysis showed that microtubules bind SYP21-positive microsomes. Transient transformation of pollen tubes with LAT52-YFP-SYP21 revealed that microtubules play a key role in the delivery of prevacuolar compartments to tubular vacuoles. The Royal Society 2018-10-31 /pmc/articles/PMC6223213/ /pubmed/30381363 http://dx.doi.org/10.1098/rsob.180078 Text en © 2018 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research
Onelli, Elisabetta
Scali, Monica
Caccianiga, Marco
Stroppa, Nadia
Morandini, Piero
Pavesi, Giulio
Moscatelli, Alessandra
Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes
title Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes
title_full Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes
title_fullStr Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes
title_full_unstemmed Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes
title_short Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes
title_sort microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6223213/
https://www.ncbi.nlm.nih.gov/pubmed/30381363
http://dx.doi.org/10.1098/rsob.180078
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