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Raman spectroscopy for medulloblastoma
PURPOSE: The aim of the study is to use Raman spectroscopy to analyze the biochemical composition of medulloblastoma and normal tissues from the safety margin of the CNS and to find specific Raman biomarkers capable of differentiating between tumorous and normal tissues. METHODS: The tissue samples...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224026/ https://www.ncbi.nlm.nih.gov/pubmed/30003328 http://dx.doi.org/10.1007/s00381-018-3906-7 |
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author | Polis, Bartosz Imiela, Anna Polis, Lech Abramczyk, Halina |
author_facet | Polis, Bartosz Imiela, Anna Polis, Lech Abramczyk, Halina |
author_sort | Polis, Bartosz |
collection | PubMed |
description | PURPOSE: The aim of the study is to use Raman spectroscopy to analyze the biochemical composition of medulloblastoma and normal tissues from the safety margin of the CNS and to find specific Raman biomarkers capable of differentiating between tumorous and normal tissues. METHODS: The tissue samples consisted of medulloblastoma (grade IV) (n = 11). The tissues from the negative margins were used as normal controls. Raman images were generated by a confocal Raman microscope—WITec alpha 300 RSA. RESULTS: Raman vibrational signatures can predict which tissue has tumorous biochemistry and can identify medulloblastoma. The Raman technique makes use of the fact that tumors contain large amounts of protein and far less lipids (fatty compounds), while healthy tissue is rich in both. CONCLUSION: The ability of Raman spectroscopy and imaging to detect medulloblastoma tumors fills the niche in diagnostics. These powerful analytical techniques are capable of monitoring tissue morphology and biochemistry. Our results demonstrate that RS can be used to discriminate between normal and medulloblastoma tissues. |
format | Online Article Text |
id | pubmed-6224026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-62240262018-11-19 Raman spectroscopy for medulloblastoma Polis, Bartosz Imiela, Anna Polis, Lech Abramczyk, Halina Childs Nerv Syst Original Paper PURPOSE: The aim of the study is to use Raman spectroscopy to analyze the biochemical composition of medulloblastoma and normal tissues from the safety margin of the CNS and to find specific Raman biomarkers capable of differentiating between tumorous and normal tissues. METHODS: The tissue samples consisted of medulloblastoma (grade IV) (n = 11). The tissues from the negative margins were used as normal controls. Raman images were generated by a confocal Raman microscope—WITec alpha 300 RSA. RESULTS: Raman vibrational signatures can predict which tissue has tumorous biochemistry and can identify medulloblastoma. The Raman technique makes use of the fact that tumors contain large amounts of protein and far less lipids (fatty compounds), while healthy tissue is rich in both. CONCLUSION: The ability of Raman spectroscopy and imaging to detect medulloblastoma tumors fills the niche in diagnostics. These powerful analytical techniques are capable of monitoring tissue morphology and biochemistry. Our results demonstrate that RS can be used to discriminate between normal and medulloblastoma tissues. Springer Berlin Heidelberg 2018-07-12 2018 /pmc/articles/PMC6224026/ /pubmed/30003328 http://dx.doi.org/10.1007/s00381-018-3906-7 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Polis, Bartosz Imiela, Anna Polis, Lech Abramczyk, Halina Raman spectroscopy for medulloblastoma |
title | Raman spectroscopy for medulloblastoma |
title_full | Raman spectroscopy for medulloblastoma |
title_fullStr | Raman spectroscopy for medulloblastoma |
title_full_unstemmed | Raman spectroscopy for medulloblastoma |
title_short | Raman spectroscopy for medulloblastoma |
title_sort | raman spectroscopy for medulloblastoma |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224026/ https://www.ncbi.nlm.nih.gov/pubmed/30003328 http://dx.doi.org/10.1007/s00381-018-3906-7 |
work_keys_str_mv | AT polisbartosz ramanspectroscopyformedulloblastoma AT imielaanna ramanspectroscopyformedulloblastoma AT polislech ramanspectroscopyformedulloblastoma AT abramczykhalina ramanspectroscopyformedulloblastoma |