Cargando…

Raman spectroscopy for medulloblastoma

PURPOSE: The aim of the study is to use Raman spectroscopy to analyze the biochemical composition of medulloblastoma and normal tissues from the safety margin of the CNS and to find specific Raman biomarkers capable of differentiating between tumorous and normal tissues. METHODS: The tissue samples...

Descripción completa

Detalles Bibliográficos
Autores principales: Polis, Bartosz, Imiela, Anna, Polis, Lech, Abramczyk, Halina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224026/
https://www.ncbi.nlm.nih.gov/pubmed/30003328
http://dx.doi.org/10.1007/s00381-018-3906-7
_version_ 1783369523831767040
author Polis, Bartosz
Imiela, Anna
Polis, Lech
Abramczyk, Halina
author_facet Polis, Bartosz
Imiela, Anna
Polis, Lech
Abramczyk, Halina
author_sort Polis, Bartosz
collection PubMed
description PURPOSE: The aim of the study is to use Raman spectroscopy to analyze the biochemical composition of medulloblastoma and normal tissues from the safety margin of the CNS and to find specific Raman biomarkers capable of differentiating between tumorous and normal tissues. METHODS: The tissue samples consisted of medulloblastoma (grade IV) (n = 11). The tissues from the negative margins were used as normal controls. Raman images were generated by a confocal Raman microscope—WITec alpha 300 RSA. RESULTS: Raman vibrational signatures can predict which tissue has tumorous biochemistry and can identify medulloblastoma. The Raman technique makes use of the fact that tumors contain large amounts of protein and far less lipids (fatty compounds), while healthy tissue is rich in both. CONCLUSION: The ability of Raman spectroscopy and imaging to detect medulloblastoma tumors fills the niche in diagnostics. These powerful analytical techniques are capable of monitoring tissue morphology and biochemistry. Our results demonstrate that RS can be used to discriminate between normal and medulloblastoma tissues.
format Online
Article
Text
id pubmed-6224026
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-62240262018-11-19 Raman spectroscopy for medulloblastoma Polis, Bartosz Imiela, Anna Polis, Lech Abramczyk, Halina Childs Nerv Syst Original Paper PURPOSE: The aim of the study is to use Raman spectroscopy to analyze the biochemical composition of medulloblastoma and normal tissues from the safety margin of the CNS and to find specific Raman biomarkers capable of differentiating between tumorous and normal tissues. METHODS: The tissue samples consisted of medulloblastoma (grade IV) (n = 11). The tissues from the negative margins were used as normal controls. Raman images were generated by a confocal Raman microscope—WITec alpha 300 RSA. RESULTS: Raman vibrational signatures can predict which tissue has tumorous biochemistry and can identify medulloblastoma. The Raman technique makes use of the fact that tumors contain large amounts of protein and far less lipids (fatty compounds), while healthy tissue is rich in both. CONCLUSION: The ability of Raman spectroscopy and imaging to detect medulloblastoma tumors fills the niche in diagnostics. These powerful analytical techniques are capable of monitoring tissue morphology and biochemistry. Our results demonstrate that RS can be used to discriminate between normal and medulloblastoma tissues. Springer Berlin Heidelberg 2018-07-12 2018 /pmc/articles/PMC6224026/ /pubmed/30003328 http://dx.doi.org/10.1007/s00381-018-3906-7 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Polis, Bartosz
Imiela, Anna
Polis, Lech
Abramczyk, Halina
Raman spectroscopy for medulloblastoma
title Raman spectroscopy for medulloblastoma
title_full Raman spectroscopy for medulloblastoma
title_fullStr Raman spectroscopy for medulloblastoma
title_full_unstemmed Raman spectroscopy for medulloblastoma
title_short Raman spectroscopy for medulloblastoma
title_sort raman spectroscopy for medulloblastoma
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224026/
https://www.ncbi.nlm.nih.gov/pubmed/30003328
http://dx.doi.org/10.1007/s00381-018-3906-7
work_keys_str_mv AT polisbartosz ramanspectroscopyformedulloblastoma
AT imielaanna ramanspectroscopyformedulloblastoma
AT polislech ramanspectroscopyformedulloblastoma
AT abramczykhalina ramanspectroscopyformedulloblastoma