Cargando…

The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans

Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282 mg/d,...

Descripción completa

Detalles Bibliográficos
Autores principales: Most, Jasper, Warnke, Ines, Boekschoten, Mark V., Jocken, Johan W. E., de Groot, Philip, Friedel, Angelika, Bendik, Igor, Goossens, Gijs H., Blaak, Ellen E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224187/
https://www.ncbi.nlm.nih.gov/pubmed/29786471
http://dx.doi.org/10.1080/21623945.2018.1469942
_version_ 1783369554760564736
author Most, Jasper
Warnke, Ines
Boekschoten, Mark V.
Jocken, Johan W. E.
de Groot, Philip
Friedel, Angelika
Bendik, Igor
Goossens, Gijs H.
Blaak, Ellen E.
author_facet Most, Jasper
Warnke, Ines
Boekschoten, Mark V.
Jocken, Johan W. E.
de Groot, Philip
Friedel, Angelika
Bendik, Igor
Goossens, Gijs H.
Blaak, Ellen E.
author_sort Most, Jasper
collection PubMed
description Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282 mg/d, 80 mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12 weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects.
format Online
Article
Text
id pubmed-6224187
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-62241872018-11-09 The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans Most, Jasper Warnke, Ines Boekschoten, Mark V. Jocken, Johan W. E. de Groot, Philip Friedel, Angelika Bendik, Igor Goossens, Gijs H. Blaak, Ellen E. Adipocyte Research Paper Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282 mg/d, 80 mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12 weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects. Taylor & Francis 2018-05-22 /pmc/articles/PMC6224187/ /pubmed/29786471 http://dx.doi.org/10.1080/21623945.2018.1469942 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Most, Jasper
Warnke, Ines
Boekschoten, Mark V.
Jocken, Johan W. E.
de Groot, Philip
Friedel, Angelika
Bendik, Igor
Goossens, Gijs H.
Blaak, Ellen E.
The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans
title The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans
title_full The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans
title_fullStr The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans
title_full_unstemmed The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans
title_short The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans
title_sort effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224187/
https://www.ncbi.nlm.nih.gov/pubmed/29786471
http://dx.doi.org/10.1080/21623945.2018.1469942
work_keys_str_mv AT mostjasper theeffectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT warnkeines theeffectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT boekschotenmarkv theeffectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT jockenjohanwe theeffectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT degrootphilip theeffectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT friedelangelika theeffectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT bendikigor theeffectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT goossensgijsh theeffectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT blaakellene theeffectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT mostjasper effectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT warnkeines effectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT boekschotenmarkv effectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT jockenjohanwe effectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT degrootphilip effectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT friedelangelika effectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT bendikigor effectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT goossensgijsh effectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans
AT blaakellene effectsofpolyphenolsupplementationonadiposetissuemorphologyandgeneexpressioninoverweightandobesehumans