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Pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation
Microcolonies are aggregates of a few dozen to a few thousand cells exhibited by many bacteria. The formation of microcolonies is a crucial step towards the formation of more mature bacterial communities known as biofilms, but also marks a significant change in bacterial physiology. Within a microco...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224386/ https://www.ncbi.nlm.nih.gov/pubmed/30410109 http://dx.doi.org/10.1038/s41598-018-34754-4 |
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author | Pönisch, Wolfram Eckenrode, Kelly B. Alzurqa, Khaled Nasrollahi, Hadi Weber, Christoph Zaburdaev, Vasily Biais, Nicolas |
author_facet | Pönisch, Wolfram Eckenrode, Kelly B. Alzurqa, Khaled Nasrollahi, Hadi Weber, Christoph Zaburdaev, Vasily Biais, Nicolas |
author_sort | Pönisch, Wolfram |
collection | PubMed |
description | Microcolonies are aggregates of a few dozen to a few thousand cells exhibited by many bacteria. The formation of microcolonies is a crucial step towards the formation of more mature bacterial communities known as biofilms, but also marks a significant change in bacterial physiology. Within a microcolony, bacteria forgo a single cell lifestyle for a communal lifestyle hallmarked by high cell density and physical interactions between cells potentially altering their behaviour. It is thus crucial to understand how initially identical single cells start to behave differently while assembling in these tight communities. Here we show that cells in the microcolonies formed by the human pathogen Neisseria gonorrhoeae (Ng) present differential motility behaviors within an hour upon colony formation. Observation of merging microcolonies and tracking of single cells within microcolonies reveal a heterogeneous motility behavior: cells close to the surface of the microcolony exhibit a much higher motility compared to cells towards the center. Numerical simulations of a biophysical model for the microcolonies at the single cell level suggest that the emergence of differential behavior within a multicellular microcolony of otherwise identical cells is of mechanical origin. It could suggest a route toward further bacterial differentiation and ultimately mature biofilms. |
format | Online Article Text |
id | pubmed-6224386 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-62243862018-11-13 Pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation Pönisch, Wolfram Eckenrode, Kelly B. Alzurqa, Khaled Nasrollahi, Hadi Weber, Christoph Zaburdaev, Vasily Biais, Nicolas Sci Rep Article Microcolonies are aggregates of a few dozen to a few thousand cells exhibited by many bacteria. The formation of microcolonies is a crucial step towards the formation of more mature bacterial communities known as biofilms, but also marks a significant change in bacterial physiology. Within a microcolony, bacteria forgo a single cell lifestyle for a communal lifestyle hallmarked by high cell density and physical interactions between cells potentially altering their behaviour. It is thus crucial to understand how initially identical single cells start to behave differently while assembling in these tight communities. Here we show that cells in the microcolonies formed by the human pathogen Neisseria gonorrhoeae (Ng) present differential motility behaviors within an hour upon colony formation. Observation of merging microcolonies and tracking of single cells within microcolonies reveal a heterogeneous motility behavior: cells close to the surface of the microcolony exhibit a much higher motility compared to cells towards the center. Numerical simulations of a biophysical model for the microcolonies at the single cell level suggest that the emergence of differential behavior within a multicellular microcolony of otherwise identical cells is of mechanical origin. It could suggest a route toward further bacterial differentiation and ultimately mature biofilms. Nature Publishing Group UK 2018-11-08 /pmc/articles/PMC6224386/ /pubmed/30410109 http://dx.doi.org/10.1038/s41598-018-34754-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pönisch, Wolfram Eckenrode, Kelly B. Alzurqa, Khaled Nasrollahi, Hadi Weber, Christoph Zaburdaev, Vasily Biais, Nicolas Pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation |
title | Pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation |
title_full | Pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation |
title_fullStr | Pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation |
title_full_unstemmed | Pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation |
title_short | Pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation |
title_sort | pili mediated intercellular forces shape heterogeneous bacterial microcolonies prior to multicellular differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224386/ https://www.ncbi.nlm.nih.gov/pubmed/30410109 http://dx.doi.org/10.1038/s41598-018-34754-4 |
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