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Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects

Accurate in vivo localisation of minimal amounts of functionalised gold-nanoparticles, enabling e.g. early-tumour diagnostics and pharmacokinetic tracking studies, requires a precision imaging system offering very high sensitivity, temporal and spatial resolution, large depth penetration, and arbitr...

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Autores principales: Grüner, Florian, Blumendorf, Florian, Schmutzler, Oliver, Staufer, Theresa, Bradbury, Michelle, Wiesner, Ulrich, Rosentreter, Tanja, Loers, Gabriele, Lutz, David, Richter, Bernadette, Fischer, Markus, Schulz, Florian, Steiner, Swantje, Warmer, Martin, Burkhardt, Anja, Meents, Alke, Kupinski, Matthew, Hoeschen, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224495/
https://www.ncbi.nlm.nih.gov/pubmed/30410002
http://dx.doi.org/10.1038/s41598-018-34925-3
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author Grüner, Florian
Blumendorf, Florian
Schmutzler, Oliver
Staufer, Theresa
Bradbury, Michelle
Wiesner, Ulrich
Rosentreter, Tanja
Loers, Gabriele
Lutz, David
Richter, Bernadette
Fischer, Markus
Schulz, Florian
Steiner, Swantje
Warmer, Martin
Burkhardt, Anja
Meents, Alke
Kupinski, Matthew
Hoeschen, Christoph
author_facet Grüner, Florian
Blumendorf, Florian
Schmutzler, Oliver
Staufer, Theresa
Bradbury, Michelle
Wiesner, Ulrich
Rosentreter, Tanja
Loers, Gabriele
Lutz, David
Richter, Bernadette
Fischer, Markus
Schulz, Florian
Steiner, Swantje
Warmer, Martin
Burkhardt, Anja
Meents, Alke
Kupinski, Matthew
Hoeschen, Christoph
author_sort Grüner, Florian
collection PubMed
description Accurate in vivo localisation of minimal amounts of functionalised gold-nanoparticles, enabling e.g. early-tumour diagnostics and pharmacokinetic tracking studies, requires a precision imaging system offering very high sensitivity, temporal and spatial resolution, large depth penetration, and arbitrarily long serial measurements. X-ray fluorescence imaging could offer such capabilities; however, its utilisation for human-sized scales is hampered by a high intrinsic background level. Here we measure and model this anisotropic background and present a spatial filtering scheme for background reduction enabling the localisation of nanoparticle-amounts as reported from small-animal tumour models. As a basic application study towards precision pharmacokinetics, we demonstrate specific localisation to sites of disease by adapting gold-nanoparticles with small targeting ligands in murine spinal cord injury models, at record sensitivity levels using sub-mm resolution. Both studies contribute to the future use of molecularly-targeted gold-nanoparticles as next-generation clinical diagnostic and pharmacokinetic tools.
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spelling pubmed-62244952018-11-13 Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects Grüner, Florian Blumendorf, Florian Schmutzler, Oliver Staufer, Theresa Bradbury, Michelle Wiesner, Ulrich Rosentreter, Tanja Loers, Gabriele Lutz, David Richter, Bernadette Fischer, Markus Schulz, Florian Steiner, Swantje Warmer, Martin Burkhardt, Anja Meents, Alke Kupinski, Matthew Hoeschen, Christoph Sci Rep Article Accurate in vivo localisation of minimal amounts of functionalised gold-nanoparticles, enabling e.g. early-tumour diagnostics and pharmacokinetic tracking studies, requires a precision imaging system offering very high sensitivity, temporal and spatial resolution, large depth penetration, and arbitrarily long serial measurements. X-ray fluorescence imaging could offer such capabilities; however, its utilisation for human-sized scales is hampered by a high intrinsic background level. Here we measure and model this anisotropic background and present a spatial filtering scheme for background reduction enabling the localisation of nanoparticle-amounts as reported from small-animal tumour models. As a basic application study towards precision pharmacokinetics, we demonstrate specific localisation to sites of disease by adapting gold-nanoparticles with small targeting ligands in murine spinal cord injury models, at record sensitivity levels using sub-mm resolution. Both studies contribute to the future use of molecularly-targeted gold-nanoparticles as next-generation clinical diagnostic and pharmacokinetic tools. Nature Publishing Group UK 2018-11-08 /pmc/articles/PMC6224495/ /pubmed/30410002 http://dx.doi.org/10.1038/s41598-018-34925-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Grüner, Florian
Blumendorf, Florian
Schmutzler, Oliver
Staufer, Theresa
Bradbury, Michelle
Wiesner, Ulrich
Rosentreter, Tanja
Loers, Gabriele
Lutz, David
Richter, Bernadette
Fischer, Markus
Schulz, Florian
Steiner, Swantje
Warmer, Martin
Burkhardt, Anja
Meents, Alke
Kupinski, Matthew
Hoeschen, Christoph
Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects
title Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects
title_full Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects
title_fullStr Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects
title_full_unstemmed Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects
title_short Localising functionalised gold-nanoparticles in murine spinal cords by X-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects
title_sort localising functionalised gold-nanoparticles in murine spinal cords by x-ray fluorescence imaging and background-reduction through spatial filtering for human-sized objects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6224495/
https://www.ncbi.nlm.nih.gov/pubmed/30410002
http://dx.doi.org/10.1038/s41598-018-34925-3
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