Cargando…
Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes
Deoxyschizandrin (DS) is a bioactive benzocyclooctadiene lignan found in the fruit of Schisandra chinensis. However, poor bioavailability and non-specificity of DS frequently caused low therapeutic efficacy. In the present study, DS-liposome (DS-lipo) was implemented to enhance the hepatic targeting...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225205/ https://www.ncbi.nlm.nih.gov/pubmed/30150602 http://dx.doi.org/10.3390/molecules23092158 |
_version_ | 1783369722363904000 |
---|---|
author | Liu, Xiaona Wang, Shifeng Wu, Zhisheng Wang, Zhaoyi Zheng, Qiusheng Li, Defang |
author_facet | Liu, Xiaona Wang, Shifeng Wu, Zhisheng Wang, Zhaoyi Zheng, Qiusheng Li, Defang |
author_sort | Liu, Xiaona |
collection | PubMed |
description | Deoxyschizandrin (DS) is a bioactive benzocyclooctadiene lignan found in the fruit of Schisandra chinensis. However, poor bioavailability and non-specificity of DS frequently caused low therapeutic efficacy. In the present study, DS-liposome (DS-lipo) was implemented to enhance the hepatic targeting and inhibition effects on adipocyte differentiation in 3T3-L1 cells. The formulations enabled encapsulation of as much as 24.14% DS. The DS-lipo prepared was about 73.08 nm, as measured by laser light scattering (LLS) morphology. In the visual field of a scanning electron microscope (SEM), the liposomes were spherical with similar size and uniform dispersion. Fluorescence live imaging study exhibited hepatic targeting of liposomes in vivo. Furthermore, High-Content Analysis (HCS) imaging microassay analyses revealed DS-lipo and DS reduced cytoplasmic lipid droplet in 3T3-L1 adipocytes, with the IC(50) value of 8.68 μM and 31.08 μM, respectively. The lipid droplet accumulation inhibition rate of 10 μM DS-lipo was above 90%, which was even superior to the effect of 30 μM DS solution. The current findings suggest that DS-lipo was a therapeutic strategy for alleviating lipid-associated diseases and nonalcoholic fatty liver disease (NAFLD). |
format | Online Article Text |
id | pubmed-6225205 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62252052018-11-13 Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes Liu, Xiaona Wang, Shifeng Wu, Zhisheng Wang, Zhaoyi Zheng, Qiusheng Li, Defang Molecules Article Deoxyschizandrin (DS) is a bioactive benzocyclooctadiene lignan found in the fruit of Schisandra chinensis. However, poor bioavailability and non-specificity of DS frequently caused low therapeutic efficacy. In the present study, DS-liposome (DS-lipo) was implemented to enhance the hepatic targeting and inhibition effects on adipocyte differentiation in 3T3-L1 cells. The formulations enabled encapsulation of as much as 24.14% DS. The DS-lipo prepared was about 73.08 nm, as measured by laser light scattering (LLS) morphology. In the visual field of a scanning electron microscope (SEM), the liposomes were spherical with similar size and uniform dispersion. Fluorescence live imaging study exhibited hepatic targeting of liposomes in vivo. Furthermore, High-Content Analysis (HCS) imaging microassay analyses revealed DS-lipo and DS reduced cytoplasmic lipid droplet in 3T3-L1 adipocytes, with the IC(50) value of 8.68 μM and 31.08 μM, respectively. The lipid droplet accumulation inhibition rate of 10 μM DS-lipo was above 90%, which was even superior to the effect of 30 μM DS solution. The current findings suggest that DS-lipo was a therapeutic strategy for alleviating lipid-associated diseases and nonalcoholic fatty liver disease (NAFLD). MDPI 2018-08-27 /pmc/articles/PMC6225205/ /pubmed/30150602 http://dx.doi.org/10.3390/molecules23092158 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Xiaona Wang, Shifeng Wu, Zhisheng Wang, Zhaoyi Zheng, Qiusheng Li, Defang Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes |
title | Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes |
title_full | Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes |
title_fullStr | Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes |
title_full_unstemmed | Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes |
title_short | Deoxyschizandrin Loaded Liposomes on the Suppression Lipid Accumulation in 3T3-L1 Adipocytes |
title_sort | deoxyschizandrin loaded liposomes on the suppression lipid accumulation in 3t3-l1 adipocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225205/ https://www.ncbi.nlm.nih.gov/pubmed/30150602 http://dx.doi.org/10.3390/molecules23092158 |
work_keys_str_mv | AT liuxiaona deoxyschizandrinloadedliposomesonthesuppressionlipidaccumulationin3t3l1adipocytes AT wangshifeng deoxyschizandrinloadedliposomesonthesuppressionlipidaccumulationin3t3l1adipocytes AT wuzhisheng deoxyschizandrinloadedliposomesonthesuppressionlipidaccumulationin3t3l1adipocytes AT wangzhaoyi deoxyschizandrinloadedliposomesonthesuppressionlipidaccumulationin3t3l1adipocytes AT zhengqiusheng deoxyschizandrinloadedliposomesonthesuppressionlipidaccumulationin3t3l1adipocytes AT lidefang deoxyschizandrinloadedliposomesonthesuppressionlipidaccumulationin3t3l1adipocytes |