Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B

Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked...

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Autores principales: Westphal, Klaus Ringsborg, Muurmann, Asmus Toftkær, Paulsen, Iben Engell, Nørgaard, Kim Tanja Hejselbak, Overgaard, Marie Lund, Dall, Sebastian Mølvang, Aalborg, Trine, Wimmer, Reinhard, Sørensen, Jens Laurids, Sondergaard, Teis Esben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225250/
https://www.ncbi.nlm.nih.gov/pubmed/30200525
http://dx.doi.org/10.3390/molecules23092232
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author Westphal, Klaus Ringsborg
Muurmann, Asmus Toftkær
Paulsen, Iben Engell
Nørgaard, Kim Tanja Hejselbak
Overgaard, Marie Lund
Dall, Sebastian Mølvang
Aalborg, Trine
Wimmer, Reinhard
Sørensen, Jens Laurids
Sondergaard, Teis Esben
author_facet Westphal, Klaus Ringsborg
Muurmann, Asmus Toftkær
Paulsen, Iben Engell
Nørgaard, Kim Tanja Hejselbak
Overgaard, Marie Lund
Dall, Sebastian Mølvang
Aalborg, Trine
Wimmer, Reinhard
Sørensen, Jens Laurids
Sondergaard, Teis Esben
author_sort Westphal, Klaus Ringsborg
collection PubMed
description Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.
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spelling pubmed-62252502018-11-13 Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B Westphal, Klaus Ringsborg Muurmann, Asmus Toftkær Paulsen, Iben Engell Nørgaard, Kim Tanja Hejselbak Overgaard, Marie Lund Dall, Sebastian Mølvang Aalborg, Trine Wimmer, Reinhard Sørensen, Jens Laurids Sondergaard, Teis Esben Molecules Communication Genome sequencing of the genus Fusarium has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the PKS8 gene in Fusarium graminearum, which was recently linked to gibepyrones in F. fujikuroi. Genomic analyses showed that PKS8 constitutes a stand-alone gene in F. graminearum and related species. Overexpression of PKS8 resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that PKS8 produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B. MDPI 2018-09-02 /pmc/articles/PMC6225250/ /pubmed/30200525 http://dx.doi.org/10.3390/molecules23092232 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Westphal, Klaus Ringsborg
Muurmann, Asmus Toftkær
Paulsen, Iben Engell
Nørgaard, Kim Tanja Hejselbak
Overgaard, Marie Lund
Dall, Sebastian Mølvang
Aalborg, Trine
Wimmer, Reinhard
Sørensen, Jens Laurids
Sondergaard, Teis Esben
Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B
title Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B
title_full Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B
title_fullStr Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B
title_full_unstemmed Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B
title_short Who Needs Neighbors? PKS8 Is a Stand-Alone Gene in Fusarium graminearum Responsible for Production of Gibepyrones and Prolipyrone B
title_sort who needs neighbors? pks8 is a stand-alone gene in fusarium graminearum responsible for production of gibepyrones and prolipyrone b
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225250/
https://www.ncbi.nlm.nih.gov/pubmed/30200525
http://dx.doi.org/10.3390/molecules23092232
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