Cargando…
The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14
ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing Toll-like receptor (TLR)2 and cluster of differentiation (CD)14 N-glycans, induces cytokine production, and promotes type 1 T helper (Th1) immunity, a process that plays an assisting r...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225408/ https://www.ncbi.nlm.nih.gov/pubmed/30216978 http://dx.doi.org/10.3390/molecules23092339 |
_version_ | 1783369769695576064 |
---|---|
author | Oliveira-Brito, Patrícia Kellen Martins Roque-Barreira, Maria Cristina da Silva, Thiago Aparecido |
author_facet | Oliveira-Brito, Patrícia Kellen Martins Roque-Barreira, Maria Cristina da Silva, Thiago Aparecido |
author_sort | Oliveira-Brito, Patrícia Kellen Martins |
collection | PubMed |
description | ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing Toll-like receptor (TLR)2 and cluster of differentiation (CD)14 N-glycans, induces cytokine production, and promotes type 1 T helper (Th1) immunity, a process that plays an assisting role in the combat against fungal infections. We recently demonstrated that ArtinM stimulates CD4(+) T cells to produce interleukin (IL)-17 through direct interaction with CD3. Here, we further investigated the effects of ArtinM on the production of IL-17 by B cell activation. We showed that ArtinM activates murine B cells, increasing IL-17 and IL-12p40 production. The direct effect of ArtinM was sufficient to induce IL-17 production in B cells, and we did not find differences in the levels of IL-17 between the B cells purified from the wild-type (WT) and knockout (KO) mice for TLR2 or CD14 in the presence of ArtinM. Thus, the effects of ArtinM on splenic B cells through carbohydrate recognition may contribute to Th17 immunity; however, the mechanism involved is not associated with the interaction of ArtinM with TLR2 and CD14. The current work represents a pioneering effort in the understanding of the induction of IL-17 by lectins in B cells. |
format | Online Article Text |
id | pubmed-6225408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62254082018-11-13 The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14 Oliveira-Brito, Patrícia Kellen Martins Roque-Barreira, Maria Cristina da Silva, Thiago Aparecido Molecules Communication ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing Toll-like receptor (TLR)2 and cluster of differentiation (CD)14 N-glycans, induces cytokine production, and promotes type 1 T helper (Th1) immunity, a process that plays an assisting role in the combat against fungal infections. We recently demonstrated that ArtinM stimulates CD4(+) T cells to produce interleukin (IL)-17 through direct interaction with CD3. Here, we further investigated the effects of ArtinM on the production of IL-17 by B cell activation. We showed that ArtinM activates murine B cells, increasing IL-17 and IL-12p40 production. The direct effect of ArtinM was sufficient to induce IL-17 production in B cells, and we did not find differences in the levels of IL-17 between the B cells purified from the wild-type (WT) and knockout (KO) mice for TLR2 or CD14 in the presence of ArtinM. Thus, the effects of ArtinM on splenic B cells through carbohydrate recognition may contribute to Th17 immunity; however, the mechanism involved is not associated with the interaction of ArtinM with TLR2 and CD14. The current work represents a pioneering effort in the understanding of the induction of IL-17 by lectins in B cells. MDPI 2018-09-13 /pmc/articles/PMC6225408/ /pubmed/30216978 http://dx.doi.org/10.3390/molecules23092339 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Oliveira-Brito, Patrícia Kellen Martins Roque-Barreira, Maria Cristina da Silva, Thiago Aparecido The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14 |
title | The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14 |
title_full | The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14 |
title_fullStr | The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14 |
title_full_unstemmed | The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14 |
title_short | The Response of IL-17-Producing B Cells to ArtinM Is Independent of Its Interaction with TLR2 and CD14 |
title_sort | response of il-17-producing b cells to artinm is independent of its interaction with tlr2 and cd14 |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225408/ https://www.ncbi.nlm.nih.gov/pubmed/30216978 http://dx.doi.org/10.3390/molecules23092339 |
work_keys_str_mv | AT oliveirabritopatriciakellenmartins theresponseofil17producingbcellstoartinmisindependentofitsinteractionwithtlr2andcd14 AT roquebarreiramariacristina theresponseofil17producingbcellstoartinmisindependentofitsinteractionwithtlr2andcd14 AT dasilvathiagoaparecido theresponseofil17producingbcellstoartinmisindependentofitsinteractionwithtlr2andcd14 AT oliveirabritopatriciakellenmartins responseofil17producingbcellstoartinmisindependentofitsinteractionwithtlr2andcd14 AT roquebarreiramariacristina responseofil17producingbcellstoartinmisindependentofitsinteractionwithtlr2andcd14 AT dasilvathiagoaparecido responseofil17producingbcellstoartinmisindependentofitsinteractionwithtlr2andcd14 |