Cargando…
Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions
A novel heterogeneous enzyme-palladium (Pd) (0) nanoparticles (PdNPs) bionanohybrid has been synthesized by an efficient, green, and straightforward methodology. A designed Geobacillus thermocatenulatus lipase (GTL) variant genetically and then chemically modified by the introduction of a tailor-mad...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225421/ https://www.ncbi.nlm.nih.gov/pubmed/30223522 http://dx.doi.org/10.3390/molecules23092358 |
_version_ | 1783369772555042816 |
---|---|
author | Lopez-Tejedor, David de las Rivas, Blanca Palomo, Jose M. |
author_facet | Lopez-Tejedor, David de las Rivas, Blanca Palomo, Jose M. |
author_sort | Lopez-Tejedor, David |
collection | PubMed |
description | A novel heterogeneous enzyme-palladium (Pd) (0) nanoparticles (PdNPs) bionanohybrid has been synthesized by an efficient, green, and straightforward methodology. A designed Geobacillus thermocatenulatus lipase (GTL) variant genetically and then chemically modified by the introduction of a tailor-made cysteine-containing complementary peptide- was used as the stabilizing and reducing agent for the in situ formation of ultra-small PdNPs nanoparticles embedded on the protein structure. This bionanohybrid was an excellent catalyst in the synthesis of trans-ethyl cinnamate by Heck reaction at 65 °C. It showed the best catalytic performance in dimethylformamide (DMF) containing 10–25% of water as a solvent but was also able to catalyze the reaction in pure DMF or with a higher amount of water as co-solvent. The recyclability and stability were excellent, maintaining more than 90% of catalytic activity after five cycles of use. |
format | Online Article Text |
id | pubmed-6225421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62254212018-11-13 Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions Lopez-Tejedor, David de las Rivas, Blanca Palomo, Jose M. Molecules Article A novel heterogeneous enzyme-palladium (Pd) (0) nanoparticles (PdNPs) bionanohybrid has been synthesized by an efficient, green, and straightforward methodology. A designed Geobacillus thermocatenulatus lipase (GTL) variant genetically and then chemically modified by the introduction of a tailor-made cysteine-containing complementary peptide- was used as the stabilizing and reducing agent for the in situ formation of ultra-small PdNPs nanoparticles embedded on the protein structure. This bionanohybrid was an excellent catalyst in the synthesis of trans-ethyl cinnamate by Heck reaction at 65 °C. It showed the best catalytic performance in dimethylformamide (DMF) containing 10–25% of water as a solvent but was also able to catalyze the reaction in pure DMF or with a higher amount of water as co-solvent. The recyclability and stability were excellent, maintaining more than 90% of catalytic activity after five cycles of use. MDPI 2018-09-14 /pmc/articles/PMC6225421/ /pubmed/30223522 http://dx.doi.org/10.3390/molecules23092358 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lopez-Tejedor, David de las Rivas, Blanca Palomo, Jose M. Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions |
title | Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions |
title_full | Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions |
title_fullStr | Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions |
title_full_unstemmed | Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions |
title_short | Ultra-Small Pd(0) Nanoparticles into a Designed Semisynthetic Lipase: An Efficient and Recyclable Heterogeneous Biohybrid Catalyst for the Heck Reaction under Mild Conditions |
title_sort | ultra-small pd(0) nanoparticles into a designed semisynthetic lipase: an efficient and recyclable heterogeneous biohybrid catalyst for the heck reaction under mild conditions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225421/ https://www.ncbi.nlm.nih.gov/pubmed/30223522 http://dx.doi.org/10.3390/molecules23092358 |
work_keys_str_mv | AT lopeztejedordavid ultrasmallpd0nanoparticlesintoadesignedsemisyntheticlipaseanefficientandrecyclableheterogeneousbiohybridcatalystfortheheckreactionundermildconditions AT delasrivasblanca ultrasmallpd0nanoparticlesintoadesignedsemisyntheticlipaseanefficientandrecyclableheterogeneousbiohybridcatalystfortheheckreactionundermildconditions AT palomojosem ultrasmallpd0nanoparticlesintoadesignedsemisyntheticlipaseanefficientandrecyclableheterogeneousbiohybridcatalystfortheheckreactionundermildconditions |