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Detection of an amplification bias associated to Leuconostocaceae family with a universal primer routinely used for monitoring microbial community structures within food products

OBJECTIVES: Sequencing of 16S rDNA V3–V4 region is widely applied for food community profiling. However, two different universal forward primers (named here MUYZER-primer1 and KLINDWORTH-primer2) targeting an identical conservative sequence upstream of the V3 region of 16S rRNA gene, and only distin...

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Detalles Bibliográficos
Autores principales:	Poirier, Simon, Rué, Olivier, Coeuret, Gwendoline, Champomier-Vergès, Marie-Christine, Loux, Valentin, Chaillou, Stéphane
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Research Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225703/ https://www.ncbi.nlm.nih.gov/pubmed/30409220 http://dx.doi.org/10.1186/s13104-018-3908-2

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