Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice

CRISPR/dCas9 is a versatile tool that can be used to recruit various effectors and fluorescent molecules to defined genome regions where it can modulate genetic and epigenetic markers, or track the chromatin dynamics in live cells. In vivo applications of CRISPR/dCas9 in animals have been challenged...

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Autores principales: Duan, Jinzhi, Lu, Guangqing, Hong, Yu, Hu, Qingtao, Mai, Xueying, Guo, Jing, Si, Xiaofang, Wang, Fengchao, Zhang, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225728/
https://www.ncbi.nlm.nih.gov/pubmed/30409154
http://dx.doi.org/10.1186/s13059-018-1530-1
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author Duan, Jinzhi
Lu, Guangqing
Hong, Yu
Hu, Qingtao
Mai, Xueying
Guo, Jing
Si, Xiaofang
Wang, Fengchao
Zhang, Yu
author_facet Duan, Jinzhi
Lu, Guangqing
Hong, Yu
Hu, Qingtao
Mai, Xueying
Guo, Jing
Si, Xiaofang
Wang, Fengchao
Zhang, Yu
author_sort Duan, Jinzhi
collection PubMed
description CRISPR/dCas9 is a versatile tool that can be used to recruit various effectors and fluorescent molecules to defined genome regions where it can modulate genetic and epigenetic markers, or track the chromatin dynamics in live cells. In vivo applications of CRISPR/dCas9 in animals have been challenged by delivery issues. We generate and characterize a mouse strain with dCas9-EGFP ubiquitously expressed in various tissues. Studying telomere dynamics in these animals reveals surprising results different from those observed in cultured cell lines. The CRISPR/dCas9 knock-in mice provide an important and versatile tool to mechanistically study genome functions in live animals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-018-1530-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-62257282018-11-19 Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice Duan, Jinzhi Lu, Guangqing Hong, Yu Hu, Qingtao Mai, Xueying Guo, Jing Si, Xiaofang Wang, Fengchao Zhang, Yu Genome Biol Short Report CRISPR/dCas9 is a versatile tool that can be used to recruit various effectors and fluorescent molecules to defined genome regions where it can modulate genetic and epigenetic markers, or track the chromatin dynamics in live cells. In vivo applications of CRISPR/dCas9 in animals have been challenged by delivery issues. We generate and characterize a mouse strain with dCas9-EGFP ubiquitously expressed in various tissues. Studying telomere dynamics in these animals reveals surprising results different from those observed in cultured cell lines. The CRISPR/dCas9 knock-in mice provide an important and versatile tool to mechanistically study genome functions in live animals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-018-1530-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-11-08 /pmc/articles/PMC6225728/ /pubmed/30409154 http://dx.doi.org/10.1186/s13059-018-1530-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Duan, Jinzhi
Lu, Guangqing
Hong, Yu
Hu, Qingtao
Mai, Xueying
Guo, Jing
Si, Xiaofang
Wang, Fengchao
Zhang, Yu
Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice
title Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice
title_full Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice
title_fullStr Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice
title_full_unstemmed Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice
title_short Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice
title_sort live imaging and tracking of genome regions in crispr/dcas9 knock-in mice
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6225728/
https://www.ncbi.nlm.nih.gov/pubmed/30409154
http://dx.doi.org/10.1186/s13059-018-1530-1
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